Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) 7.0 Standards, Policies, References, and Resources 7.1 Standards 7.2 Policies 7.2.1 Health and Safety Manual (PUB-3000) Chapters 7.2.2 Other Biosafety-related LBNL Publications 7.3 References 7.4 Resources Appendix A Glossary Appendices Appendix B Pathogen and Toxin Lists B.1 Introduction and Scope B.2 NIH Guidelines Human Etiologic Agents B.2.1 Risk Group 1 Agents B.2.2 Risk Group 2 Agents B.2.3 Risk Group 3 Agents B.2.4 Risk Group 4 Agents B.2.5 Animal Viral Etiologic Agents in Common Use B.2.6 Murine Retroviral Vectors B.3 Select Agents and Toxins B.4 Plant Pathogens B.4.1 Plant Pathogen Bacteria (by Scientific Name) B.4.2 Plant Pathogen Fungi (by Scientific Name) B.4.3 Plant Pathogen Viruses (Regulated by the State of California) Appendix C Laboratory Biosafety Level 1 and 2 Criteria C.1 Introduction and Scope C.2 Laboratory Biosafety Level 1 C.2.1 BL1 Standard Microbiological Practices C.2.2 BL1 Special Practices C.2.3 BL1 Safety Equipment (Primary Barriers and Personal Protective Equipment) C.2.4 BL1 Laboratory Facilities (Secondary Barriers) C.3 Laboratory Biosafety Level 2 C.3.1 BL2 Standard Microbiological Practices C.3.2 BL2 Special Practices C.3.3 BL2 Safety Equipment (Primary Barriers and Personal Protective Equipment33) C.3.4 BL2 Laboratory Facilities (Secondary Barriers) Appendix D Good Microbiological Practice D.1 Introduction and Scope D.2 Good Microbiological Practice D.2.1 Aseptic Technique D.2.2 Personal Hygiene and Dress D.2.3 Area Cleanliness and Organization Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 4
Biosafety Manual IBC-approved version (May 18, 2010) D.2.4 Biosafety Cabinets and Airborne Contamination D.2.5 Manipulation Techniques for Minimizing Aerosols D.2.6 Worker Qualifications D.2.7 Microbial Contamination Checks D.3 References Appendix E Appendix F Biosafety Cabinets E.1 Introduction and Scope E.2 Biosafety Cabinet Classifications E.2.1 Class I Biosafety Cabinet E.2.2 Class II Biosafety Cabinet E.2.3 Class III Biosafety Cabinet E.2.4 Clean Benches (Not BSCs) E.3 Biosafety Cabinet Work Practices and Procedures E.3.1 Preparing for BSC Work E.3.2 Material Placement inside the BSC E.3.3 Operations within a Class II BSC E.4 Biosafety Cabinet Decontamination and Moves E.4.1 Cabinet Surface Decontamination E.4.2 Internal Cabinet Gaseous Decontamination E.5 Biosafety Cabinet Installation and Engineering E.6 Biosafety Cabinet Testing and Certification Decontamination and Antimicrobials F.1 Introduction and Scope F.2 Decontamination Principles and Terms F.2.1 Decontamination Processes and Levels F.2.1.1 Sterilization F.2.1.2 Disinfection F.2.1.3 Sanitization F.2.1.4 Antisepsis F.2.2 Antimicrobial Categories F.2.3 Antimicrobial Selection and Registered Disinfectants F.3 Chemical Antimicrobials F.3.1 Surfactants (Soaps and Detergents) F.3.1.1 Soaps F.3.1.2 Detergents and Quaternary Ammonium Compounds F.3.2 Halogens (Chlorine and Iodine) F.3.2.1 Chlorine and Sodium Hypochlorite F.3.2.2 Iodine and Iodophors F.3.3 Alcohols F.3.4 Phenol and Phenol Derivatives (Phenolics) F.3.5 Oxidizing Agents (Hydrogen Peroxide) F.3.6 Alkylating Agents (Formaldehyde, Glutaraldehyde, Ethylene Oxide) F.3.6.1 Formaldehyde F.3.6.2 Glutaraldehyde F.3.6.3 Ethylene Oxide F.4 Physical Antimicrobials Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 5
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