Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) Present at LBNL Biosafety Level (BL) Table 8 Laboratory Biosafety Containment Levels Biosafety Level Definition yes 1 BL1 is suitable for work involving agents of unknown or minimal potential hazard to laboratory personnel and the environment, 1 or work with defined and characterized strains of viable microorganisms not known to consistently cause disease in healthy adult humans. 2 2 BL2 is suitable for laboratory work involving agents of moderate potential hazard to personnel and the environment. 1 Primary hazards to personnel working with these agents relate to accidental percutaneous or mucous membrane exposures, or ingestion of infectious materials. 2 no 3 BL3 is applicable to facilities in which work is conducted with indigenous or exotic agents that may cause serious or potentially lethal disease as a result of exposure by the inhalation route. 3 4 BL4 is required for work with dangerous and exotic agents that pose a high individual risk of life-threatening disease, aerosol transmission, or related agents having an unknown transmission risk. 3 Footnotes: 1 NIH Guidelines 2 BMBL, fifth edition, Section III 3 BMBL, fifth edition, Section IV When developing the Biosafety Work Authorization, the appropriate laboratory BL must be selected after conducting the risk assessment. Typical BLs used for various materials and agents are listed in Sections 4.4.1.1 and 4.4.1.2 of this manual. The final BL(s) are determined by the IBC. 4.4.1.1 Laboratory Biosafety Level 1 BL1 laboratories are not necessarily separated from the general traffic patterns in the building. Work is typically conducted on open benchtops using standard microbiological practices. Special containment equipment or facility design is not required, but may be used as determined by a risk assessment. Laboratory personnel must have specific training in the procedures conducted in the laboratory and must be supervised by a scientist trained in microbiology or a related science. Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 34
Biosafety Manual IBC-approved version (May 18, 2010) Laboratory Biosafety Level 1 work with open benchtops and standard microbiological practices. Source: LBNL EH&S. The BL will be determined as part of the risk assessment. BL1 containment is typically required for laboratory work involving: • biological agents that meet the definition of Risk Group (RG) 1 (i.e., agents not associated with disease in healthy adult humans); • biological materials not suspected of containing RG2 or higher agents in a quantity or form that may cause human disease (e.g., many soils and nonprimate animal cells); • biological agents or materials not characterized by the supplier as RG2 or higher; • transgenic or wild-type laboratory animals that have size or growth requirements allowing the use of containment for laboratory animals (e.g., rodents) and are o free of zoonotic diseases, and o not infected with, implanted with, or containing RG2 or higher agents or materials; • laboratory growth of nongreenhouse transgenic plants (see Section 4.4.3 of this manual); • biological agents, materials, or animals not typically categorized as RG2 or BL2 (or higher) as detailed Section 4.4.1.2 of this manual. 4.4.1.2 Laboratory Biosafety Level 2 BL2 laboratories follow BL1 requirements and additional BL2 requirements such as: • Laboratory personnel have specific training in handling any pathogenic agents and are supervised by scientists competent in handling infectious agents and associated procedures. • Access to the laboratory is restricted when work is being conducted. • All procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets (BSCs) or other physical containment equipment. The BL will be determined as part of the risk assessment. Laboratory BL2 containment is typically required for laboratory work involving: • biological agents categorized as RG2 in the NIH Guidelines or by the supplier; • uses of biological agents described as BL2 in BMBL agent summary statements or other BMBL; • biological materials that may contain RG2 agents (e.g., sewage); Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 35
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