Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) F.4.1 Heat F.4.1.1 Dry Heat (Baking and Incineration) F.4.1.2 Wet Heat (Boiling and Autoclaving) F.4.2 Ultraviolet (UV) Radiation F.4.2.1 UV Light Health Effects and Categories F.4.2.2 Biosafety Cabinet UV Light F.4.3 Ionizing Radiation F.4.4 Visible Light F.4.5 Filtration (HEPA Filters) F.5 Autoclave Sterilization and Safety F.5.1 Autoclaves and Sterilization F.5.2 Autoclave Operation and Safety F.5.2.1 Autoclave Instruction F.5.2.2 Autoclave Operation Modes F.5.2.3 Autoclave Container Selection F.5.2.4 Autoclave Preparation and Loading F.5.2.5 Autoclave Cycle and Time Selection F.5.2.6 Removing Autoclave Loads F.5.2.7 Autoclave Material Staging F.5.2.8 Burn Emergencies F.5.3 Autoclave Maintenance and Monitoring F.6 References Appendix G Biological Spills and Cleanup G.1 Introduction and Scope G.2 Precleanup Considerations G.3 Biological Spill outside a Biosafety Cabinet G.4 Biohazardous Spill inside a Biosafety Cabinet G.5 Centrifuge Malfunction or Spill G.6 Radioactive and Biohazardous Spill G.7 Chemical and Biohazardous Spill G.8 Cleanup of Small Dead Animals, Nests, or Droppings Appendix H Transportation and Shipping H.1 Introduction and Scope H.2 How to Determine Transportation Mode and Requirements H.3 Requirements and Processes for Receiving, Transporting, and Shipping H.3.1 Employee Transportation of Materials H.3.1.1 Hand-Carry Transport between Laboratories H.3.1.2 Hand-Carry Transport between Buildings H.3.1.3 Personal Transportation in a Motor Vehicle H.3.1.4 Personal Transportation on an LBNL Bus H.3.2 LBNL Receiving, Transportation, and Shipping H.3.2.1 LBNL Receiving H.3.2.2 LBNL Transportation H.3.2.3 LBNL and Common Carrier Shipping H.4 Unregulated and Regulated Materials H.4.1 Unregulated Biological Materials H.4.2 Regulated Biological Materials H.5 References and Resources Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 6
Biosafety Manual IBC-approved version (May 18, 2010) Appendix I Import, Export, and Transfer Restrictions I.1 Introduction and Scope I.2 Importing or Transfer into the U.S. and California I.2.1 I.2.2 CDC and APHIS Select Agent and Toxin Restrictions APHIS Agricultural Permits I.2.2.1 APHIS Plant Health Permits I.2.2.2 APHIS Animal Health Permits I.2.2.3 APHIS Genetically Engineered Organisms Permits I.2.3 CDC Agents or Vectors of Human Disease Permits I.2.4 Food and Drug Administration Import Program I.2.5 Fish and Wildlife Service Permits I.3 Exporting or Transfer from the U.S. I.3.1 Commerce Control List I.3.2 U.S. Munitions List I.3.3 Biological Weapons Convention Lists I.4 References Tables Table 1 Guidance for Starting and Conducting Work Table 2 Risk Group Classification Table 3 Pathogenic Agent and Toxin Categories Table 4 Materials Regulated by USDA-APHIS Table 5 Materials Covered by the OSHA Bloodborne Pathogens Standard Table 6 Examples of Zoonotic and Other Diseases Related to Animals Table 7 Equipment Hazard Examples Table 8 Laboratory Biosafety Containment Levels Table 9 Additional Containment Categories Table 10 Biosafety-Related Training Courses Table 11 Decontamination Processes, Levels, and Antimicrobial Examples Table B-1 National Select Agent Registry Select Agents and Toxins Table B-2 Additional Information for National Select Agent Registry Toxins Table E-1 Protection Offered by Classes of Biosafety Cabinets Table E-2 Characteristics of Biosafety Cabinet Classes Table F-1 Levels of Chemical Disinfection Table F-2 Activity Levels of Selected Liquid Germicides Table F-3 Descending Order of Organism Resistance to Germicidal Chemicals Table H-1 Transportation Modes and Biological Materials Not Allowed Table I-1 Wildlife or Plant Specimens That May Require an FWS Permit Table I-2 Commerce Control List of Biological Agents Table I-3 BWC Agents Not On the Commerce Control List Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 7
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