Maternal variation in Huichol and Mixtec populations from Mexico

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American Southwest indicating recent expansion in this region. It is hypothesied that later migrations and expansions have blurred the pre-existing mtDNA structure, at least in this region, and provides us to see preexisting mtDNA structure (Kemp et al., 2010). Today inhabitants of Mexico and Mesoamerica could be divided into mainly two distinct groups: mestizos (individuals with recent admixed ancestry) and native indigenous groups. Although mestizos represent nearly 95% of total population, the Native American component is highly prevalent in their gene pool. It varies by region depending of recent postcolonisational demographic history, but generally maternal component could be up to 100% Native American while paternal is mostly European. 14
2. EXPERIMENTAL STUDY 2.1 Aims of this study This study is a part of the TÜMRI Evolution Department co project together with Dr. Eske Willerslev from the Copenhagen laboratory. The aim of the project to research human ancient remains, found in New Mexico, which are representatives of Clovis culture. Our partners make an ancient DNA (aDNA) analysis, using autosomal and mitochondrial DNA. The mission of our laboratory is to create a base system for the research for future data comparison. This includes the high-resolution analysis of mtDNA and autosomal chromosomes of Mexico extant populations. aDNA researches are still in progress, and the results are not yet available. The aim of the present study is to describe the mtDNA variety of two indigenous populations from Middle America, Huichols and Mixtecs: 1. To determine mitochondrial haplogroups and haplotypes. 2. To compare these populations with the published data and describe them in the context of maternal genetic variation of Middle America. 3. To make a full sequence analysis for possibly interesting Native American haplotypes. 15
Page 1 and 2: TARTU UNIVERSITY FACULTY OF SCIENCE
Page 3 and 4: ABBREVIATIONS bp - base pair D-loop
Page 5 and 6: 1. LITERATURE OVERVIEW 1.1 Mitochon
Page 7 and 8: One of the reasons may be, that ped
Page 9 and 10: Figure 2. Diagnostic markers of Nat
Page 11 and 12: According to it, only one small gro
Page 13: their way down to the South America
Page 17 and 18: To determine possible contamination
Page 19 and 20: * let dry at 37ºC for 5 min *add 1
Page 21 and 22: 2.3 RESULTS AND DISCUSSION 2.3.1 Ph
Page 23 and 24: that populations have gone through
Page 25 and 26: Figure 5. Haplogroup A haplotype ne
Page 27 and 28: Figure 7. Haplogroup C haplotype ne
Page 29 and 30: 2.3.2 C4c haplogroup One Huichol in
Page 31 and 32: N 12705s 16223 R 73 11719s R0 14766
Page 33 and 34: CONCLUSIONS Mitochondrial DNA has b
Page 35 and 36: mitokondriaalne DNA. Huitšoli proo
Page 37 and 38: References Achilli, A, Perego, U. A
Page 39 and 40: Forster, P., Torroni, A., Renfrew,
Page 41 and 42: Loogvali, E. L., Kivisild, T., Marg
Page 43 and 44: Sandoval, K., Buentello-Malo, L., P
Page 45 and 46: SUPPLEMENTARY MATERIALS Tabel 1.1.
Page 47 and 48: Table 4.Data used for haplogroups f

Maternal variation in Huichol and Mixtec populations from Mexico

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