Extraction Technologies For Medicinal And Aromatic Plants - Unido

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EXTRACTION TECHNOLOGIES FOR MEDICINAL AND AROMATIC PLANTS
Scale up to desired column size to meet throughput and
load objectives
Pool fractions of comparable purity and rerun if necessary
Check fraction purity using an analytical column
11.8 Applications: Natural Products Isolation
A few examples of prep-HPLC for the isolation of natural products
are summarized herein. First, tannins from Guiera seregalensis can be
isolated using the following conditions:
Column RP-18e (250 x 10 mm i.d.)
Mobile phase Water:methanol:THF (90:10:0.25) and (80:20:0.25)
Flow rate 2.5 ml/min
Detection 280 nm
Compounds isolated Galloylquinic acids, namely, 3-O-, 5-O-, 1,3-di-O-, 3,4-di-O-,
3,5-di-O-, 4,5-di-O-, 1,3,4-tri-O-, 3,4,5-tri-O- and
1,3,4,5-tetra-O-galloylquinic acid
Flavonoids from Lychnophora ericoides require the following conditions:
Columns Silica (250 x 10 mm i.d.) and ODS (250 x 10 mm i.d.)
Mobile phase Water:methanol:THF
Flow rate 2.5 ml/min
Detection 280/225 nm
Compounds isolated 7,4’-dihydroxy-fl avonol; 5,7-dihydroxy-3-methoxy-fl avonol;
galangine, 7,4’-dihydroxy-dihydrofl avono l,5,7,4’-trihydroxydihydrofl
avonol,7-hydroxy-4’-methoxy-dihydrofl avonol;
pinobanksin, 5,7’-dihydroxy-4’-hydroxy-fl avanone; 7-hydroxy-
4’-methoxy-fl avanone; 5,7-dihydroxy-fl avone; acacetin;
7-hydroxy-3’,4’-dihydroxy-isofl avone; 15-desoxigoiazensolide,
2’,3’-dihydro-15-desoxygoyazensolide; eremantholides A and
C; 4,5-dihydroeremantholide A and lychnopholide
For the isolation of peptide components of bacitracin, use:
Column C8 (250 x 16 mm i.d.)
Mobile phase Gradient acetonitrile, methanol and phosphate buffer
Flow rate 9.0 ml/min
Detection 254 nm
Compounds isolated Peptides
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