SOP â Malaria Microscopy - NVBDCP

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Quality Assurance of Malaria Diagnostic tests7.1.8 Handling of the compound microscopeThe microscope is an instrument of precision and care must be taken to preserve itsaccuracy, So, precautions have to be taken to keep the microscope and lens system clean.There are only a few ABSOLUTE rules to observe in caring for the microscopes.Please report any malfunctions immediately to your supervisor.• Always use two hands to carry the scope -one on the arm and one under the base -no exceptions! Never carry the microscope upside down, for the ocular can and willfall out.• Use lens paper to clean all the lenses before start of laboratory work and after usingthe oil immersion lens. Do not ever use anything other than lens paper to clean thelenses. Other papers are too impure and will scratch the optical coating on thelenses.• Always remove oil from the oil-immersion objective after its use, with lens paperlightly moistened with alcohol.• Always use the proper focussing technique to avoid ramming the objective lens into aslide. This can break the objective lens and/or ruin an precious slide.• Always turn off the light when not using the scope.• Always carefully place the electric wires out of harm’s way. Wires looped in the legspaces invite a major microscope disaster. Try sliding the wire down through thedrawer handles by the side of your bench space.• Avoid attack of dust and water to prevent fungal contamination.• Never allow the objective lens to touch the cover glass or the slide. Never touch thelenses,• Keep the stage of the microscope clean and dry.• Do not tilt the microscope when working with oil immersion system.• Never lower the body tube with the coarse adjustment while you are looking throughthe microscope.• Never exchange the objective or oculars of different microscopes.7.1.9 Care of the microscopeProvided normal care and common sense are exercised, the laboratory microscope will beuseful for many years.7.1.9.1Removing dust and grease• when not in use during the day, keep the microscope covered with a clean cloth orplastic cover to protect the lenses from dust that settles out of the air. Overnight, or ifit is to remain unused for long periods, place the microscope inside it’s box with thedoor tightly closed.• to protect the objective lenses, rotate the 10x objective to line up with the ocular. Oiland grease from eyelashes and fingers are easily deposited on lenses and ocularsas the microscope is used. Clean these parts with lens tissue or with very soft cottoncloth.• clean the oil immersion objective after use. If it is not cleaned, the oil will harden andmake the objective useless. A lens tissue or soft cotton cloth is usually sufficient forthe purpose. However, never use this tissue or cloth to clean other objectives, theoculars or the mirror, otherwise oil will be transferred to these components.SOP – Malaria Microscopy© Copyright to Dte. NVBDCP Only. Any modification are prohibited
Quality Assurance of Malaria Diagnostic tests7.1.9.2 Preventing the growth of fungusIn warm, humid climates, it is very easy for fungal growths to become established on lensesand prisms. These growths can create problems and may even become so bad that themicroscope can no longer be used. The lenses may need to be re-polished by themanufacturer, which is very expensive and may take several months.Fungus cannot grow on glass when the atmosphere is dry and therefore store themicroscope in a dry atmosphere when it is not being used.To avoid fungal growth, use a desiccant like silica gel, with the ability to absorb water vapourfrom the air. Self-indicating silica gel is blue when active but becomes pink when it hasabsorbed all the water. It can then be reactivated by heating.7.1.10 Transporting the microscopeWhen the microscope is to be transported from one location to another, ensure that it isproperly secured inside its box. The best way to do this is by means of the securing device,which screws into the base of the microscope.SOP – Malaria Microscopy© Copyright to Dte. NVBDCP Only. Any modification are prohibited
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