SOP – Malaria Microscopy - NVBDCP

Quality Assurance of Malaria Diagnostic testsNATIONAL VECTOR BORNE DISEASE CONTROL PROGRAMME (NVBDCP)STANDARD OPERATING PROCEDURE FOR QASOP TitleGENERAL QUALITY ASSURANCE - CLEANING ANDMAINTENANCE OF GLASSWARESOP No. SOP G.04 Revision No. 0.0 Effective Date Dec. 2007Replacement No Dated Page No.Next Review onMaximum 2 years from “effective date”SOP G 04 - CLEANING AND MAINTENANCE OF GLASSWARE7.4.1 PurposeThis SOP describes the method for cleaning and maintenance of the glassware used formalaria microscopy and different ways to accomplish it.7.4.2 PrincipleThere has to be a minimum level of good laboratory practices which should be maintained atthe laboratories under National QA Programme. Cleaning and maintenance of the glasswareused for malaria microscopy is one of the basic requirements to achieve the qualitylaboratory results.7.4.3 Materials required and procedures for cleaning of micro glass slides for cleaningmicro glass slides the following materials are required• a large plastic basin• gauze or cotton wool• a good quality detergent (powder or liquid)• 2-4 clean, dry, lint-free cotton cloths• clean water.7.4.3.1 Micro glass slidesMicroscope slides are usually supplied in boxes of 50 or 72. It may be described on the boxas “washed” or “pre-cleaned”, but the slides will still need to be properly washed, dried andwrapped. It is not possible to make good quality blood films on dirty microscope slides.Blood films made on dirty or greasy slides will wash off easily during staining. It is thereforebest to discard slides that have an iridescent bloom or appear white or opaque and are notproperly cleaned or slides from old stock with surface scratches or chipped edges.7.4.4 Process for cleaningSOP – Malaria Microscopy© Copyright to Dte. NVBDCP Only. Any modification are prohibited