SOP â Malaria Microscopy - NVBDCP

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Quality Assurance of Malaria Diagnostic tests8.5.3.1.1 Results• the results of cross-checking by the Reference Laboratories in the Form 09should be sent to the concerned DMOs by the 15 th of the succeeding monthwith a copy to the state and Dte. of NVDBCP. Dte. of NVBDCP envisages useof NAMMIS for transmission of cross- checking of results as well.• the district would pass on the results to the PHCs during the monthly reviewmeeting which is held in each district every month.• the states would compile the data of each district and send to the Dte. ofNVBDCP.• in case of high discrepancy rate i.e., 2% or above, the cross-checkinglaboratories would take the needful remedial action.• there will be supervision of the concerned laboratory to find the condition ofthe microscope and to provide hands on training to the concerned LT (s).• these remedial actions would be taken in consultation with the stateprogramme officer.Proforma given at Form No 09 would be used for cross-checking of slides by thedesignated reference laboratories.8.5.3.1 Regional Referral LaboratoriesThe slides cross checked at Regional Referral Laboratories ( ROH&FW/ZMO/ICMR insts)shall be cross checked by National Referral Laboratory to maintain the quality of crosschecking.• This process shall be twice in a year. Once slides examined in the PHCs duringtransmission period and subsequently cross checked at RRLs. Another duringpre/post transmission period.• Every fouth positive slides and tenth negative slides received from the PHC shallbe send by RRLs after cross checking for re-cross checking by NIMR.• NIMR shall convey the months to the RRLs for sending the slides cross checkedby them as the transmission period is not uniform acroos the country.SOP – Malaria Microscopy© Copyright to Dte. NVBDCP Only. Any modification are prohibited
Quality Assurance of Malaria Diagnostic testsNATIONAL VECTOR BORNE DISEASE CONTROL PROGRAMME (NVBDCP)STANDARD OPERATING PROCEDURE FOR QASOP TitlePREPARATION OF QA PANEL SLIDES FOR EQASSOP No. SOP: M 06 Revision No. 0.0 Effective Date Dec. 2007Replacement No Dated Page No.Next Review onMaximum 2 years from “effective date”SOP: M 06- PREPARATION OF QA PANEL SLIDES FOR EQAS8.6.1 PurposeThis SOP describes the preparation of Malaria positive blood smears of known diagnosticcomposition, for conducting EQA.8.6.2 ObjectivesThe validated and coded blood smears for malaria microscopy will be prepared by theidentified external institution for testing the proficiency of the LTs and accuracy in expressingresults.8.6.3 ProcedureOne of the important responsibilities of the National Reference Laboratory (National Instituteof Malaria Reseach) is to prepare the QA panel slides for conducting EQAS. The slides willbe prepared by the following steps:• prepare blood smears from malaria positive patients, following the methodalready described in SOP M. 01.• collect 5ml blood by venepuncture from patients found to be malaria positive(donor’s venepuncture: patients found to be malaria positive collect 5 ml bloodafter obtaining consent).• on an average, prepare 50 slides from each donor’s blood and stain in separatebatches to avoid cross contamination during the preparation, following theprocedures at SOP M. 03.• each slide will have of both thick and thin smear.• stain slides with JSB stain and protect by permanent mounting with cover slip.• take proper care to mount the slides free from air bubbles.• label the slides with a code and ID number.• determine the consensus diagnosis by experienced microscopists from NIMR foreach patient/donor and record/report the results as positive or negative formalaria, the species and the parasite density for upper and lower limit.• fifty (50) sets of twenty (20) slides, i.e. one thousand (1000); each set containing8 P. falciparum, 4 P. vivax, 4 P. falciparum + P. vivax mixed infection and 4malaria negative slides will be provided for assessing the competence andproficiency of LTs in malaria diagnosis, species identification and densitydetermination.• to accommodate the anticipated high demand, the distribution of these sets ofslides will be on a time-restricted manner twice a year.SOP – Malaria Microscopy© Copyright to Dte. NVBDCP Only. Any modification are prohibited
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SOP â Malaria Microscopy - NVBDCP

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SOP â Malaria Microscopy - NVBDCP