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Untitled - CNR

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Marine research at <strong>CNR</strong>Table 1 Main biochemical characteristics of the polychaete Sabella spallanzanii and macroalgaeSabella spallanzaniiWater (%) 79 ± 0.41Total proteins (mg/ml) 26 ± 0.21Total lipids (mg/ml) 7 ± 0.002Triglycerides (mg/ml) 0.40 ± 0.0003Cholesterol (mg/ml) 0.32 ± 0.0002Phospholipids (mg/ml ) 0.84 ± 0.0004∑ Saturated fatty acids (SAFAs) 70.28± 0.42∑ Monounsaturated fatty acids (MUFAs) 13.65± 0.22∑ Polyunsaturated fatty acids (PUFAs) 16,07± 0.31ω-3 fatty acids 4.5 ± 0.22ω-3/ω-6 fatty acid ratio 0.5 ± 0.27Macroalgae - Fatty acids %14:0 7.69 ± 2.4416:0 41.27 ± 12.5318:0 4.42 ± 2.8718:1ω9 5.94 ± 3.3518:2ω6 3.11 ± 4.1618:3ω3 1.13 ± 0.6418:3ω6 2.79 ± 2.6520:4ω6 5.45 ± 3.5420:5ω3 4.54 ± 5.022:6ω3 2.27 ± 0.91∑ Saturated fatty acids (SAFAs) 58.61 ± 13.39∑ Monounsaturated fatty acids (MUFAs) 18.52 ± 9.30∑ Polyunsaturated fatty acids (PUFAs) 22.92 ± 10.35ω-3/ω-6 fatty acid ratio 1.04 ± 0.47Table 1: Main biochemical characteristics of the polychaete Sabella spallanzanii andmacroalgae.of chicken egg white.In cnidarians an antibacterial activity wasrecorded in the nematocyst extracts of P.axinellae, C. auriantiaca and A. aequina.SEM observations in P. axinellae showed alytic mode of action of nematocyst extractson treated bacteria (Figure 4). The proteinsinvolved in the antimicrobial activityexerted by the marine invertebrates werethen chemically characterized and resultedinfluenced by the same physical-chemicalparameters (ionic strength, pH, and temperature)as other lysozyme molecules.By disc diffusion assay, used to assessthe antimicrobial activity, we observed thatamong the examined macroalgae, the lipidicextracts of G. longissima, C. barbataand C. rupestris, containing mainlythe palmitic acid (as revealed by the gaschromatography),showed a bacteriostaticactivity against several Vibrio species (Figure2d).2049

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