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Chapter 5 Genetic Analysis of Apomixis - cimmyt

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VIIITables4 Table 1.15 Table 1.29 Table 2.19 Table 2.29 Table 2.310 Table 2.411 Table 2.511 Table 2.612 Table 2.714 Table 2.815 Table 2.936 Table 3.172 Table 5.1100 Table 7.1124 Table 9.1125 Table 9.2132 Table 9.3141 Table 10.1142 Table 10.2144 Table 10.3145 Table 10.4157 Table 11.1159 Table 11.2162 Table 11.3162 Table 11.4163 Table u.s163 Table 11.6233 Table 14.1234 Table 14.2International Agricultural Research CentersInstitutions facilitating the application <strong>of</strong> biotechnology to internationalagriculture<strong>Genetic</strong> constitution <strong>of</strong> progeny from apomictic plantsSize <strong>of</strong> four categories defined in Table 2.1 for two PaniClim maximum clones (fromCombes 1975)Size <strong>of</strong> four categories defined in Table 2.1 for three types <strong>of</strong> progeny involvingtwo PartlIeU/1I11l species. Adapted from Powers and Rollins (1945)Estimation <strong>of</strong> apomixis rate and categories <strong>of</strong> progeny from chromosome countsand isozyme analyses <strong>of</strong> Tripsacum populations (Berthaud et aI., unpublished data)Variation in chromosome number for progeny from wild populations <strong>of</strong> Tripsacumdactyloides mexicanllll1. (Seeds were collected in the wild.)Size <strong>of</strong> categories defined in Table 2.1 for two Pennisetum flnccidum x P mezianumcrosses. From Bashaw et al. (1992)Distribution <strong>of</strong> clones in Tripsacum wild population "La Toma"Distribution <strong>of</strong> clones according to ploidy level from the P maximum collectionestablished in Cote d'lvoire (Combes 1975)Distribution <strong>of</strong> species <strong>of</strong> Paspalltm according to their incompatibility system,ploidy level, and meiosis behavior (from studies at lEONE, Quarin, personalcomm.)Refractive index (n ) D<strong>of</strong> common and potential clearing mediaSegregations for mode <strong>of</strong> reproduction in 10 crosses <strong>of</strong> Panicltm maximum (Savidan1981; Savidan et al. 1989)Phylogenetic, genomic, and developmental peculiarities that hypotheses for thegenetic regulation <strong>of</strong> apomixis and related reproductive anomalies must explainThe four theoretical <strong>of</strong>fspring classes in progenies from facultative pseudogamousapomictsMain characteristics <strong>of</strong> megasporogenesis and megagametogenesis during bothsexual reproduction and gametophytic apomixisAdvantages and disadvantages <strong>of</strong> important procedures for the investigation <strong>of</strong>modes <strong>of</strong> reproduction at the plant and progeny levelsAgronomic evaluation <strong>of</strong> Brachiaria accessions in BrazilMode <strong>of</strong> reproduction <strong>of</strong> 15 species <strong>of</strong> Brachiaria, based on embryo-sac analysisSegregation for mode <strong>of</strong> reproduction in Brachiaria hybridsComparison between progeny test and embryo-sac analysis for determination <strong>of</strong>mode <strong>of</strong> reproduction for first-generation interspecific Brachiaria hybridsCrossabilities between maize and wild Tripsacum species and presumed naturalinterspecific hybrids 0Crossabilities between pearl millet and three apomictic wild Pennisetum speciesFacultativeness <strong>of</strong> apomixis and diplospory rate in the Tripsacum accession used inthe backcross transfer <strong>of</strong> apomixis into maize and three BC Iprogenies, showingvariation for this rateChromosome numbers <strong>of</strong> BC I(2n =56) progenies as estimated by flow cytometryMaize x Tripsacum Be 3progenies, in which the BC 4s are the n + n categoryMaize x Tripsacum BC 4with known mode <strong>of</strong> reproductionExamples <strong>of</strong> isolated genes and their promoters that might be useful as tools for denovo syntb.~sis<strong>of</strong> the apomixis trait in sexual cropsExamples <strong>of</strong> patents linked with the engineering <strong>of</strong> the apomixis trait in sexualcrops

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