Chapter 5 Genetic Analysis of Apomixis - cimmyt

(lanilkoll.. of Apomktlt MeUotb... 434. The method works also for meristems,floral primordia, and other generalsubjects where one wants to see only thecell walls. Oxalate crystals and opalinesilica survive the procedure intact. If theepidermis separates from the mesophyll,use less heat next time during fixation.AcknowledgmentsThe author would like to thank Drs. David M.Stelly and H. J. Price for their help during thewriting of this chapter. This research wassupported by Texas Advanced Technology andResearch Program Grant 999902090.ReferencesBer~n, G. P, and 1 PMiksche. 1976. Botonical Mierotechnique ondCytochemistry. Ames, Iowa: The lowo State Unive~ify Press.Crone, C. F. 1978. Apomixis and crossing incompatibilities in someZephyrantheoe. Ph.D. dissertation, Ihe Unive~ify of Texas ot Austin,Austin, Texos.Crane, C. F., and J. G. Carman. 1987. Mechanisms of apamixis in Bymusredisetus fram eastern Australia and New Zealand. Americon. Journolof Botony. 74: 477-96.&delska, 0., H. K. Galle, H. H. Heunert, and K. Philipp. 1971.Somenonloge und fruhe Endospermenlwicklung von Josione mantonoIComponumceae). Insmuf fUr den Wissenschahlichen Film.Erde~ka, 0., H. H. Heunert, T. Hard, J. Kaeding, and H. WllImonn. 1979.Be!ruchtung und fruhe Enlwidclung von Embryo und Endosperm beimSchneeglikkchen.lnstiluf fiir den Wissensmahlichen Film, no. 1465.Fredrikson, M. 1990. Embryological Sludy of Herminium monorchis(Orchidaceae) using conlocal scanning loser mi