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<strong>Undergrad</strong>uate Research at UMass Dartmouth<br />

31<br />

Fifteen mature basil plants were purchased, numbered,<br />

and transplanted into larger pots. Plants<br />

1–7 were planted traditionally, upright (displayed in<br />

Figure 1a.), and plants 8–15 were planted in pots<br />

fashioned so that the plant would hang upside down<br />

(displayed in Figure 1b.). Stalks that had a second<br />

set of true leaves, and sufficient space between the<br />

pairs to make a cut, were pruned.<br />

After four weeks of growth, it was observed that<br />

stems of upright plants that had been pruned on<br />

Day 1 had established pairs of offshoot stems with<br />

two or three sets of leaves. Stems of upside-down<br />

plants that had been pruned on Day 1 had established<br />

pairs of offshoot stems with only one or two<br />

sets of leaves. This suggests that the upright plants<br />

experienced increased growth compared to the<br />

upside-down plants. Figures 2. and 3 display this<br />

growth difference.<br />

Several obstacles were encountered in trying to<br />

maintain healthy plants. Challenges included:<br />

growing basil during the late winter/early spring<br />

months (which is <strong>no</strong>t basil’s typical growing season<br />

for this region), securing an indoor location that met<br />

the environmental needs of basil, and the presence<br />

of insects.<br />

Due to the complications with maintaining consistently<br />

healthy plants, <strong>no</strong> formal measurements<br />

with the experimental plants have been taken at<br />

this time, but there have been several practice<br />

measurements including extracting chlorophyll and<br />

measuring the wavelengths with a spectrophotometer,<br />

staining stem cross sections with toluidine blue<br />

and observing the plant vasculature under a microscope,<br />

and experimenting with different grip set-ups<br />

for tensile testing. Images of the practice stained<br />

samples are shown in Figure 4.<br />

Figure 1a. The upright basil plants<br />

Figure 1b. Some of the upside down basil plants on a garment rack

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