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Selected Projects 20<strong>16</strong>-<strong>18</strong><br />
Left: Front <strong>View</strong> of Setup with 22C Bin<br />
Plate Arrangement of 22C Bin<br />
were used to grow the biofilms. At specified times, the<br />
plates were treated with the UV light with one of the<br />
three duration times (1, 10, or 20 minutes) and then<br />
placed back in the bin to continue growth. Triplicate<br />
plates were included for each treatment. Samples<br />
were then collected from the treated and <strong>no</strong>n-treated<br />
areas (one and two weeks after the UV treatment) to<br />
be analyzed at a later date. Samples were collected<br />
to investigate presence of chlorophyll a (representing<br />
microalgal abundance) and abundances of bacteria<br />
on the surfaces. A second experiment was conducted<br />
with bacterial mixed cultures in one temperature only<br />
and a 1-week post-treatment incubation.<br />
am continuing to complete the bacterial abundance<br />
counts over the next few months.<br />
Overall, the UV device appeared to be successful<br />
in killing existing biofilm and slowing down<br />
regrowth in the already formed biofilms. The<br />
observations show that we were successful in<br />
creating artificial marine biofilms in the lab and<br />
demonstrate the effectiveness of the UV device on<br />
these biofilms, mirroring overall results from pilot<br />
experiments conducted by Moisander lab and<br />
the BEC collaborators with natural biofilms from<br />
Buzzards Bay in 20<strong>16</strong>.<br />
54<br />
By the end of the summer, all samples were collected<br />
for each analysis and experiments completed. I also<br />
finished the analysis of all chlorophyll samples using<br />
fluorometry, and started the bacterial counts using<br />
epifluorescence microscope. The data compilation<br />
for chlorophyll data is currently in progress, and I<br />
My research experience was very eye-opening<br />
regarding where and how I want to work in my<br />
future research career. I thoroughly enjoyed coming<br />
up with an experimental design and tackling<br />
the research challenges with Dr. Pia Moisander,<br />
as well as seeing the project come to a success-
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