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SZENT-GYÖRGYI STUDENTS<br />
TAMÁS FÜZESI<br />
Szeged Scientists Academy, 1 st year<br />
University of Szeged,<br />
Faculty of Medicine, 1 st year<br />
YEAR OF BIRTH:<br />
<strong>19</strong>99<br />
FORMER<br />
SZENT-GYÖRGYI<br />
PUPIL:<br />
yes<br />
SZENT-GYÖRGYI<br />
MENTOR:<br />
Tamás Martinek<br />
SPECIALIZATION:<br />
molecular biology, genetics<br />
SECONDARY SCHOOL:<br />
Bethlen Gábor<br />
Református Gimnázium<br />
és Szathmáry Kollégium,<br />
Hódmezővásárhely<br />
NAME OF TEACHER:<br />
Henriett Jóriné Csölle<br />
LANGUAGES:<br />
English/intermediate<br />
IMPORTANCE, AIMS AND POSSIBLE OUTCOME OF RESEARCH<br />
There is a pressing need for opening up ways for therapeutic macromolecules to the<br />
intracellular targets. Certain viral and bacterial proteins are readily internalized in functional<br />
form through caveolar endocytosis, but mimicking this process with protein cargos at<br />
therapeutically relevant concentrations is a great challenge. Our research group’s goal is<br />
to target certain gangliosides, as key receptors triggering endocytosis in the caveolar pits,<br />
which can internalize large cargos in functional form without degradation. Previously,<br />
we identified a pentapeptide sequence that specifically captured the glycan moiety of<br />
a ganglioside. The peptide-tag facilitated caveolar endocytosis of proteins so that the<br />
cargo-loaded caveosomes did not fuse with lysosomes. An immunoglobulin G complex<br />
(580 kDa) was successfully delivered into live HeLa cells at a low nanomolar extracellular<br />
concentration, and the escape of the functional cargo protein to the cytosol was observed.<br />
Our current work focuses on the intracellular delivery of proteins with a specific function<br />
into knock-out cell lines, restoring cell phsyiology. Instead of fluorescent detection, we<br />
express the protein, decorate it with our peptide-tag, then we perform functional tests on<br />
the human cells.<br />
AMBITIONS AND CAREER GOALS<br />
During my work I would like to learn as many molecular biological methods as possible<br />
especially the techniques with DNA and proteins which are the keystones of molecular<br />
biological researches. Furthermore, our research group’s aim is to develop a drug delivery<br />
technology which can be a milestone in the application of protein-based drugs. My<br />
personal aim in the Scientists Academy is to acquire significant theoretical and practical<br />
knowledge which can not be learnt elsewhere and become a professional who is effective<br />
in clinical work and science alike.<br />
HONORS AND PRIZES<br />
• OKTV Biology 32 nd place<br />
PUBLICATIONS<br />
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103
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