SzSA YearBook 2018/19

SZENT-GYÖRGYI JUNIOR MENTORS
GYÖNGYI ILONA CINEGE
Immunology Unit,
Institute of Genetics,
Biological Research Center of the
Hungarian Academy of Sciences
Address: Temesvári krt. 62., H-6726 Szeged, Hungary
RESEARCH AREA
Analysis of the Nimrod gene cluster in the model organism,
Drosophila melanogaster. The cluster carries genes which
encode proteins having role in the innate immune
responses, as bacterial binding, phagocytosis, moreover
composition of the first barrier, the cuticle. Furthermore,
in the species of the ananassae subgroup following
parasitoid wasp infection, multinucleated giant hemocytes
(MGH) are synthesized, which kill with high efficiency the
parasitoid larvae by encapsulation. We analyze the proteins
expressed in the MGH, to better understand the effective
killing procedure. We hope that our studies will serve as a
granuloma model.
TECHNIQUES AVAILABLE IN THE LAB
DNA preparation, construction of plasmids, generation
of transgenic fly stocks, indirect immuno fluorescence,
western blot, immunoprecipitation, immuno histochemistry,
preparation of fluorophore labeled bacteria,
phagocytosis, RNA isolation, RT-PCR, development of
monoclonal antibodies.
SELECTED PUBLICATIONS
Kari, B., Csordás, G., Honti, V., Cinege, G., Williams, M.J., Andó,
I., Kurucz, É. (2016) The raspberry gene is involved in the
regulation of the cellular immune response in drosophila
melanogaster. PLoS One 11: e0150910.
Márkus, R., Lerner, Z., Honti, V., Csordás, G., Zsámboki, J.,
Cinege, G., Párducz, Á., Lukacsovich, T., Kurucz, É., Andó, I.
(2015) Multinucleated giant hemocytes are effector cells in
cell-mediated immune responses of drosophila. J Innate
Immun 7: 340-53.
Honti, V., Cinege, G., Csordás, G., Kurucz, É., Zsámboki, J.,
Evans, C.J., Banerjee, U., Andó, I. (2013) Variation of NimC1
expression in Drosophila stocks and transgenic strains. FLY
7: 263-266.
Cinege, G., Louis, S., Hänsch, R., Schnitzler, J.P. (2009)
Regulation of isoprene synthase promoter by environmental
and internal factors. Plant Mol Biol 69: 593-604.
Cinege, G., Kereszt, A., Kertész, S., Balogh, G., Dusha I. (2003)
The roles of the different regions of the CycH protein in
c-type cytochrome biogenesis in Sinorhizobium meliloti.
Mol Genet Genomics 271: 171-179.
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