SzSA YearBook 2018/19

SZENT-GYÖRGYI MENTORS
PETRA HARTMANN
University of Szeged,
Institute of Surgical Research
Address: Pulz utca 1., H-6724 Szeged, Hungary
RESEARCH AREA
Previous studies constructed a comprehensive picture on
the biological effects of exogenous methane (CH 4
) in various
ischemia-reperfusion (IR) and inflammatory settings.
However, the underlying mechanisms are still not known
with certainty; and the immediate challenge is therefore
to test hypotheses to understand the background of CH 4
-
induced reactions. Our main purpose is to examine the
effects of CH 4
on possible cellular and subcellular targets,
including mitochondria and to apply CH 4
in complex, in vivo
animal models of IR, focusing on specific organ functions.
TECHNIQUES AVAILABLE IN THE LAB
In vivo animal models (rodents and large animals), in
vivo imaging techniques with fluorescent laser-scanning
endomicroscopy or intravital microscopy. Microcirculatory
measurements with orthogonal polarization spectral
imaging and laser-Doppler. Ex vivo organ perfusion
systems (liver, kidney) for static and dynamic graft
preservation. Measurement of mitochondrial respiration,
hydrogen-peroxyde production and membrane potential
using high-resolution respirometry and its fluorescent
moduls (Oxygraph2k). Basic laboratory techniques
(spectrophotometry, isolation of mitochondria).
SELECTED PUBLICATIONS
Szilágyi, Á.L., Mátrai, P., Hegyi, P., Tuboly, E., Pécz, D.,
Garami, A., Solymár, M., Pétervári, E., Balaskó, M., Veres, G.,
Czopf, L., Wobbe, B., Szabó, D., Wagner, J., Hartmann, P.
(2018) Compared efficacy of preservation solutions on the
outcome of liver transplantation: Meta-analysis. World J
Gastroenterol. 28;24(16):1812-1824.
Mészáros, A.T., Szilágyi, Á.L., Juhász, L., Tuboly, E., Érces, D.,
Varga, G., Hartmann, P. (2017) Mitochondria As Sources and
Targets of Methane. Front Med (Lausanne) 13;4:195
Tuboly, E., Molnár, R., Tőkés, T., Turányi, R.N., Hartmann,
P., Mészáros, A.T., Strifler, G., Földesi, I., Siska, A., Szabó, A.,
Mohácsi, Á., Szabó, G., Boros, M. (2017) Excessive alcohol
consumption induces methane production in humans and
rats. Sci Rep. 4;7(1):7329.
Strifler, G., Tuboly, E., Görbe, A., Boros, M., Pécz, D., Hartmann,
P. (2016) Targeting Mitochondrial Dysfunction with L-Alpha
Glycerylphosphorylcholine. PLoS One. 18;11(11):e0166682.
Strifler, G., Tuboly, E., Szél, E., Kaszonyi, E., Cao, C., Kaszaki,
J., Mészáros, A., Boros, M., Hartmann, P. (2016) Inhaled
Methane Limits the Mitochondrial Electron Transport
Chain Dysfunction during Experimental Liver Ischemia-
Reperfusion Injury. PLoS One. 7;11(1):e0146363.
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