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ANNUAL REPORT - Department of Biotechnology

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analyzed for the selection <strong>of</strong> best substrate for<br />

growth. Different carbon source like propionate,<br />

octanoic and decanoic acid were used as sole<br />

source <strong>of</strong> carbon in the nutrient deficient media to<br />

check for PHA production. A combination <strong>of</strong> glucose<br />

and propionate was found to be the best substrate, in<br />

which most <strong>of</strong> the selected isolates showed good<br />

growth.<br />

Bioconversion <strong>of</strong> solid waste and Biomethanation<br />

Fisheries College and Research Institute,<br />

Thoothikkudi in Tamil Nadu performed<br />

Vermicomposting <strong>of</strong> seafood processing waste. The<br />

seafood processing wastes, bulking agents and<br />

inoculum cow dung slurry or microbes<br />

Trichoderma/Azotobactor were mixed<br />

homogeneously in different ratios. Composting<br />

methods were employed for 21-25 days. Compost<br />

was then subjected to vermicomposting by using<br />

exotic earthworm Eudrilus eugeniae for 25 days<br />

which led to odourless, nitrogen rich biocompost. A<br />

biocompost yard is under construction.<br />

Self mixing Anaerobic Reactor Technology (SMART)<br />

has been developed and patented by IICT,<br />

Hyderabad and ANGRAU, Hyderabad for High Rate<br />

Biomethanation from poultry litter. The process<br />

consists <strong>of</strong> feed preparation system, two number <strong>of</strong><br />

Self Mixing Anaerobic Reactor (SMAR) in series with<br />

solid liquid separation system. The study revealed<br />

that approximately 60% <strong>of</strong> volatile solids in the litter<br />

3<br />

could be removed yielding 0.33m CH 4/(kg<br />

VS<br />

reduced) <strong>of</strong> methane at 20 days <strong>of</strong> residence time.<br />

This design is efficient as it has low residence time<br />

and methane produced could be used for brooding<br />

within the farm saving LPG and dried bio-manure<br />

could be sold as fertilizer.<br />

Bioremediation<br />

University <strong>of</strong> Rajasthan, Jaipur has identified the<br />

most tolerant plant species for the development <strong>of</strong><br />

Ecotechnology for distillary waste treatment. 9<br />

terrestrial (7 glycophytes and 4 halophytes) and 5<br />

marshy species were studied in spent wash in<br />

DBT Annual Report 2006-07<br />

126<br />

different seasons under two types <strong>of</strong> irrigation<br />

practices at field capacity viz. surface and subsurface.<br />

Plant growth was quantified in terms <strong>of</strong><br />

Schematic Flow diagram <strong>of</strong> high rage Bioemthanation <strong>of</strong> poultry litter<br />

based on smart<br />

Hight rate Biomethanation <strong>of</strong> Poultry litter : bench scale studies<br />

conducted at IICT<br />

biomass, chlorophyll and proline contents. Important<br />

findings are:<br />

Marshy species :<br />

It has been observed that Phargmites is the most<br />

tolerant species followed by Arundo and Typha ,<br />

whereas Scirpus species were the least tolerant.<br />

During the study period, identification <strong>of</strong><br />

economically important species such as henna<br />

(Lawsonia in cosmetics), Acacia (for fodder and

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