ANNUAL REPORT - Department of Biotechnology

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ecorded 50 times batch run without any loss of efficiency of conversion rate. In another study, potential of black tea and its constituents in reversal of multidrug resistance and as bio-enhancer are being studied. The non-toxic concentrations of tea polyphenols were found to effectively reverse the drug resistance as evident by p-glycoprotein expression (through western blotting) and increased drug uptake of doxorubicin (through flow cytometry). Potential of tea polyphenols as bioenhancer in cancer chemoprevention studies, experiments on hepato-carcinogenesis and skin carcinogenesis were undertaken revealed that polyphenolic constituents of both varieties of tea, green and black are providing significant protection against cancer induction in vivo models. c) Enhanced Omega-3 fatty acid content in foods: Alpha linolenic acid (ALA) is an essential omega-3 fatty acid required in the diet that gets converted in the body to Eicosapentaenoic acid and Docosahexaenoic acid that form precursors respectively for eicosanoids and membrane components especially, brain and retina. Many microorganisms can desaturate the abundant plant linoleic acid (LA) to ALA that is poorly present in plant oils. Work to develop recombinant yeast strains capable of converting LA to ALA extracellularly was carried out. More than 100 yeast isolates were collected, mostly from the premises of plant oil mills from different parts of the country, and grouped into forty independent types based on colony and cellular characteristics. Genomic DNA was isolated from the forty different strains and used for screening of omega-3 desaturase gene by PCR. Primers were designed based on sequence homology of a published yeast omega-3 desaturase with those present in algae, fungi and plants. When compared across different phylogenetic groups there is over all poor sequence similarity (23%-29%), with the exception of cyanobacetria and Arabidopsis which showed 45%-50% similarity. Three aminoacid clusters, 16-22 aa long with 45%-50% similarity among all the known desatuarse, was used for designing primers. Seven out of forty samples yielded ~600 bp amplicon. These were further confirmed by PCR using a different set of primers and 2 DNA hybridization studies. The PCR positive strains were able convert LA to ALA. d) Beta-carotene from Alga: A total of 272 salt pans seawater samples were collected along the coasts of Andhra Pradesh and Tamil Nadu. Most of the samples contained the quadriflagellate alga like Tetraselmis. Only a few samples known to contain a mixture of naked green algal species Dunaliella, thus indicating the rare occurrence of the species. The algal colonies appeared after 20 days were isolated and maintained. Dunaliella strains were investigated for their growth study and total carotenoids and potential strains have been identified for enhanced beta carotene production. In another study, carotenoids and fatty acid composition of some selected Indian brown and red seaweeds were analysed and phenolic content and antioxidant activity of extracts from seaweeds were also analysed. The brown seaweeds analysed included Sargassum marginatum, Padina tetrastromatic and Turbinaria connoides; while, the red seaweeds included Acanthophora spicifera, Euchema kappaphycus and Gracilaria folifera. The lipid content in various seaweeds varied between 1.0 and 3.0% (dwb) with P. tetrasromatica showing the highest content. Glycolipids were found to be a major lipid class, followed by neutral and phospholipids, in brown as well as red seaweeds. Brown seaweeds were found to contain fucoxanthin as the major pigment. Fucoxanthin content of fraction obtained by column chromatography was found to be >90%. Among the various solvent fractions obtained, butanolic fraction of E. kappaphycus was found to contain the highest. The red seaweeds showed relatively higher antioxidant activity than brown seaweeds as indicated by radical scavenging activity. e) Microbial Production of Nicotinamide: Nicotinamide is one of the important vitamins B3, which is mainly used in pellagra and niacin deficiency. It also has an antioxidant and cytoprotective effect. The other form of vitamin B3 is nicotinic acid, which is equally important as nicotinamide. In this project a large number of microbes have been screened for both nicotinamide and nicotinic acid production. The nitrile hydratase activity of these strains was determined by assaying the enzyme activity. The HPLC method was developed to determine 3-cyanopyridine, nicotinamide and nicotinic acid. Good nitrile 145 Bioprocess and Product Development
hydratase activity was shown by eight strains among the different strains screened for this purpose. Reactions conditions are being optimized with the isolated strains. f) Folate Supplementation: The study investigated the optimal level of folate supplementation in the presence and absence of Vit. B12 using rat model. In the first protocol, there were three treatment groups (12% protein with folate supplementation at 2, 4 & 8 mg/kg diet) and one control group (18% protein). There was no difference observed in brain weights of pups from different groups at birth. In contrast, males pups from both 4 and 8 mg folic acid supplemented groups had significantly lower (p
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ANNUAL REPORT 2006 - 2007 Departmen
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CONTENTS Chapter-1: An Overview 1 A
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Chapter-6: Biotechnology for Societ
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An Overview Biotechnology is a set
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In a project to identify, map and t
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Medicinal and Aromatic Plants Four
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wax related gene fragments having h
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esearch; appropriate regulatory mec
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Science or Food Science & Technolog
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and the National Research Centre Ca
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many sophisticated instrumentation
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Advisory Structure Standing Advisor
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Human Resource Development The Depa
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a rapidly advancing area and teache
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per year for a period of three year
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this fellowship and 9 students have
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80 70 60 50 40 30 20 10 0 1600 1400
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throughput molecular marker service
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plasmamembrane localisation of GFP-
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antibiotic biosynthesis in Streptom
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the reporting period. Out of these,
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in progress. Besides multiplication
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primers (Operon) under the series O
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a total of 326 cotyledonary node ex
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Magnaportha grisea, showed signific
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some diseases are the two most impo
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For utilization of Multiple Aleuron
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BGA transformations, MPS transforma
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development of root knot nematode,
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plant growth and development. Due t
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significant weed control at seedlin
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three rice accessions with enhanced
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oth adults and nymphs. First year m
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c-DNA probes were developed using a
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across all the 462 clones with allo
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R & D programme have also been supp
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Inauguration of Butterfly Park by S
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Germlasing of NAPs conversed in gen
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patchouli were found to be similar
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With a view to isolate the taxol bi
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attributed to the ribosome inactiva
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(RBSS) assay. Study was supported a
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showed sign of spoilage even after
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ody hepatitis (IBH) in domestic fow
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FSH, IGF-1 and cumulus granulosa mo
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uffalo UTMP cDNA exhibited 94.5, 88
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Biosurfactant Work on screening of
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conotoxins along with the posttrans
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Oligonucleotide probe for monitorin
Page 101 and 102: similarity with the already reporte
Page 103 and 104: studies on expressed sequences of t
Page 105 and 106: factors. Scientists at Sree Chitra
Page 107 and 108: A study was implemented at NCCS, Pu
Page 109 and 110: Medical Biotechnology Health relate
Page 111 and 112: F S C # C e lls 1000 800 600 400 20
Page 113 and 114: the pathogenesis of JE. It has been
Page 115 and 116: Delhi. The protocol for 4-colour wh
Page 117 and 118: Cross Sectional Morphology of the T
Page 119 and 120: Virus Research Centers or Networks
Page 121 and 122: As the clinical trials ae planned t
Page 123 and 124: een implemented for both basic and
Page 125 and 126: low cost for rapid diagnosis of dis
Page 127 and 128: Under the multi-centric project on
Page 129 and 130: The genetic basis of Type2 diabetes
Page 131 and 132: Floor model RBC Reactor - Close-up
Page 133 and 134: analyzed for the selection of best
Page 135 and 136: yield of hydrogen in the first stag
Page 137 and 138: Operational guidelines for collecti
Page 140 and 141: Programmes for SC and ST Population
Page 142 and 143: section in the Western Dun Valley t
Page 144 and 145: aromatic grasses. These women are g
Page 146 and 147: Poultry and Livestock Farming In an
Page 148: conducted on mushroom cultivation,
Page 151: probiotic selected strains of Lacto
Page 155 and 156: At Birla Institute for Scientific R
Page 157 and 158: study at Osmania University and IIC
Page 159 and 160: DBT Annual Report 2006-07 152
Page 161 and 162: In the area of recombinant pharma s
Page 163 and 164: MEC met five times during the year
Page 166 and 167: Biotechnology Information System Ne
Page 168 and 169: Bioinformatics National Certificati
Page 170 and 171: Meetings 1) International Conferenc
Page 172 and 173: Biotechnology Parks and Incubators
Page 174 and 175: International Collaboration Interna
Page 176 and 177: elow: Contraceptive and Reproductiv
Page 178 and 179: USP method. These included 59 smear
Page 180 and 181: Danforth); others (IHBT & possibly
Page 182: Memorandum of Agreement between Dep
Page 185 and 186: cytokine-mediated regulation of int
Page 187 and 188: In stem cell research, the studies
Page 189 and 190: two pathogens in the acidic environ
Page 191 and 192: software developed by TCS with supp
Page 193 and 194: eleased from activated microglia ar
Page 195 and 196: Genetic linkage mapping in Catharan
Page 197 and 198: three of the BACs (374Kb) of chromo
Page 199 and 200: ATPase mRNA binding protein, cathep
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Public Sector Undertaking Bharat Im
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complete protection against a paras
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Administration and Finance Administ
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2. As the Department wants the comm
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GENDER BUDGETING CELL The Departmen
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6. Prof. K. Dharmalingam Member Hea
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19. Prof. Mahtab S. Bamji Member Em
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INTER-DISCIPLINARY RESEARCH COMMITT
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MONITORING-CUM-EVALUATION COMMITTEE
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Dr. Debi P. Sarkar, Professor & Hea
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TASK FORCE ON BIOTECHNOLOGY BASED P
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TASK FORCE ON AGRICULTURAL BIOTECHN
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Prof. H. Sharat Chandra, Director,
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NATIONAL BIORESOURCE DEVELOPMENT BO
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Scientist 'F' Forest Research Insti
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Prof. B. N. Johri Department of Bio
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Annexure-III DBT SPONSORED UNIVERSI
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16. University of Jammu, 10 JNU-CET
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32. Visva-Bharati, 10 JNU-CET Prof
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46. Banaras Hindu 12 JNU-CET Prof.
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LONG TERM 237 Annexure-IV BIOTECHNO
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239 Annexures
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241 Annexures
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243 Annexures
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245 Annexures
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SOFTWARE AVAILABLE WITH BTISNet CEN
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249 Annexures
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12. Jagadish N, Rana R, Mishra D, G
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42. Vats D, Vishwakarma RA, Bhattac
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15. Singh, S., Upadhyay, A.K., Ajay
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PATENTS FILED / SEALED Dr. V. P. Ka
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22. Johny S, Kanginakudru S, Murali
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11. Mallappa, C., Yadav, V., Negi,
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Annexure-X STATEMENT OF BUDGET ESTI
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GST H2O2 HAU HCH hck HCMI HDAC1 HIC
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SATB1 SDL SFC SGOT SGPGI SGPT SH Si
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ANNUAL REPORT - Department of Biotechnology

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