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ANNUAL REPORT - Department of Biotechnology

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significant weed control at seedling stage particularly<br />

in seed function and germination. These<br />

mycoherbicidal agents have been successfully mass<br />

produced through solid substrate fermentation<br />

employing maize cob wastes and when applied to the<br />

seedling <strong>of</strong> the target caused more than 90%<br />

mortality. Cell free culture filtrates <strong>of</strong> these agents<br />

have also shown significant seed mortality.<br />

Purification and characterization <strong>of</strong> these byproducts<br />

are under progress.<br />

Large-scale demonstration on management <strong>of</strong><br />

Parthenium through integrated approach is being<br />

done at NRCWS, Jabalpur. Few plant species were<br />

found competitive against Parthenium. Cassia tora<br />

was found to be the best plant as it replaces<br />

Parthenium infected site during rainy season.<br />

Introductory releases <strong>of</strong> bioagants, Mexican beetle,<br />

Zygogramma bicolorata at newer sites, confirm its<br />

establishment, which reflected its success in<br />

integrated management programme. Good quality<br />

A view <strong>of</strong> field visit <strong>of</strong> trainees doing practical during<br />

Parthenium Training Programme.<br />

Parthenium compost was also prepared by pit<br />

method with low inputs. Parthenium compost along<br />

with 50% NPK gave higher yield than FYM and 50%<br />

NPK suggest its use in compost.<br />

Development <strong>of</strong> comprehensive website on biopesticide.<br />

A comprehensive website on “Bio-pesticides” has<br />

been developed which highlights the achievements<br />

made in major programmes supported by the<br />

<strong>Department</strong> on biological control <strong>of</strong> pests, disease<br />

DBT Annual Report 2006-07<br />

56<br />

and weeds. Salient achievements compiled from<br />

more than 124 completed projects have been<br />

included which will benefit scientists, entrepreneurs<br />

and farmers. The website will be launched shortly.<br />

Toxicological data generation<br />

In order to facilitate the commercialization <strong>of</strong><br />

biopesticides, the department has taken a proactive<br />

step <strong>of</strong> the generation <strong>of</strong> toxicological data <strong>of</strong><br />

potential biopesticides. In the first phase, 10<br />

biopesticides have been taken up for generation <strong>of</strong><br />

toxicological data both for primary cultures as well as<br />

for their formulations. Data have been generated for<br />

almost all the biopesticides by the two identified<br />

centers viz; ITRC, Lucknow and RRL, Jammu.<br />

Patents<br />

Patents have been filed for the mass production<br />

technologies <strong>of</strong> various biocontrol agents viz.<br />

Nomuraea rileyi, Trichoderma virens, two effective<br />

bi<strong>of</strong>ormulations <strong>of</strong> B. bassiana, Verticillium lecanii<br />

and Myrotheceium verucarria. Two patents for the<br />

products viz. Biowiltex (Trichoderma harzianum),<br />

Bionem x (Pochonia chlamydoisporia) and Biocomp<br />

x (Pseudomonas fluorescens) have been filed in<br />

India and USA through DBT patent cell.<br />

Technologies transferred and products marketed<br />

Several mass production technologies <strong>of</strong> biocontrol<br />

agents/biopesticides have been developed and<br />

standardized. Mass production technology <strong>of</strong><br />

Trichoderma viride (fermentation based) has been<br />

transferred to M/s Prathista Industries Ltd. in<br />

Nalgonda, Andhra Pradesh and M/s Haryana<br />

Biotech, Gurgaon. They have filed application for<br />

registration <strong>of</strong> their products and procured funds from<br />

the Technology Development Board (TDB).<br />

Registration has been granted to the product<br />

Bi<strong>of</strong>ungicide to M/s Prathista Industries Ltd. and the<br />

product has been launched in the market as<br />

“Protect”. Cost effective mass multiplication <strong>of</strong><br />

Trichoderma and Pseudomonas and their mixed<br />

formulation on FYM and chicken manure have been<br />

developed at GBPUAT, Pantnagar and this<br />

technology has been transferred to the farmers <strong>of</strong><br />

Uttrankhand and Uttar Pradesh as a<br />

recommendation from University as well as State<br />

<strong>Department</strong> <strong>of</strong> Agriculture.

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