ANNUAL REPORT - Department of Biotechnology

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Rural Bioresource Complex: Five Rural Bioresource Complexes have been set up at University of Agricultural Sciences, Bangalore; Haryana Agricultural University, Hisar; G.B. Pant University of Agriculture and Technology, Pant Nagar and Marathwada Agricultural University, Parbhani and Orissa University of Agriculture & Technology, Orissa with the basic objective of economic empowerment of the target group through income generation and better employment opportunities along with entrepreneurship development, social benefits and environmental sustainability. In the Rural Bioresource Complex at UAS, Bangalore, inaugurated by Dr. V. L. Chopra, Member Planning Commission. 17 types of interventions were identified and 15 of them are being promoted in the RBRC project area at Tubagere in Doddaballapur taluk of Bangalore District involving the 6,067 families. The major interventions promoted were seed productions in Ragi and Groundnut, commercial production of Pop corn, Baby corn, Tissue culture banana, Rose, Watermelon, Drumstick, Mulberry, Improved fish farming and Value added products in ragi In order to effectively implement the interventions 42 training programmes and two study tours were organized. Market linkages are established to sell the produce of growers at a profitable price. At MAU, Parbhani, base line survey has been completed. Seed production programme has been taken on 271.6 hectare areas covering 678 beneficiaries directly. The intervention on pomegranate cultivation (Bhagva variety) was completed on 75 acres area benefiting 75 farmers. The goat keeping intervention was extended to 40 landless beneficiaries by providing them most sturdy Osmanabadi goats after vaccination and deworming, 25% of the total beneficiaries have reported birth of single or twins among two-three goats. For Poultry farming (Vanraja Variety), a low cost completely prefabricated poultry shed was designed and developed and is now under mass construction on the site of 40 landless beneficiaries. The shed is designed to 69 accommodate 100 poultry birds. Till date, 843 families are direct beneficiaries and 2297 are indirect beneficiaries. Medicinal and Aromatic Plants A programme on biotechnological intervention on medicinal and aromatic plants was continued for conservation, characterization, micropropagation, production of secondary metabolites, development of standardized herbal products, isolation and characterization of novel therapeutic agents, genomics and metabolic engineering. A network project on developing standardized herbal product(s) for bovine mastitis has been initiated. The salient achievements of the programme during the year are as follows: National Gene Bank for Medicinal and Aromatic Plants The four gene banks have been further strengthened with emphasis on collection, conservation and characterization: Tropical Botanic Garden and Research Institute, Thiruvananthapuram A total of 370 accessions belonging to 96 species were collected from Kerala, Karnataka, Tamil Nadu, Goa, Andhra Pradesh and Andaman Islands and introduced in to field gene bank. Morphological characterization based on qualitative and quantitative characters of 51 accessions belonging to 7 species and anatomical characterization of 10 accessions of two species were carried out. Seeds of 19 target species were collected and deposited to the seed bank. Seven accessions belonging to three species were added in to the in vitro bank. Zygotic embryo cryopreservation was standardized in Coscinium fenestratum. Molecular characterization of 24 accessions of Costus speciosus and six accessions of Trichopus zeylanicus using RAPD and ISSR markers has been completed. Research and Development
Germlasing of NAPs conversed in gene bank at TBGRI, Thiruvenanthpuram. 1. Abrus precatorius- an accession from Andaman Island 2. Rauvolfia serpentina- a potential accession from Tamilnadu 3. Accessions of Bacopa monnieri 1. Cryopreserved zygotic embryo of Coscinium fenestratum showing germination and development 2. Shoot tips of Kaempferia galanga cryopreserved through vitrification showing recovery and development DBT Annual Report 2006-07 70 species conserved in this mode. Seed bank has been enriched by 96 accessions and now comprises of 2286 accessions belonging to 418 species. Morphological characterization of various accessions of Matricaria chamomilla (62), Foeniculum vulgare (38), Withania somnifera (121) and Papaver somniferum (350) has been carried out. Thirty four accessions of W. somnifera have been chemically evaluated for Withaferin-A contents. Representative cultures of some new germ plasm accessions of MAPs been added to tissue bank at CIMAP. 1- Chlorophytum arundinaceum; 2- Aloe vera; 3- Centella aisatica; 4- Acorus calamus; 5- Indigofera sp.; 6- Chrysanthemum cineraraefolium; 7- Berginia sp; 8- Hypericum himalaicus; 9- Swertia chirayita; 10- Paris polyphylla; 11- Salvia scalarea; 12- Gentiana kurrooa; 13- lavendula officinalis. Regional Research Laboratory, Jammu Field gene bank has been enriched by addition of 27
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ANNUAL REPORT 2006 - 2007 Departmen
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CONTENTS Chapter-1: An Overview 1 A
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Chapter-6: Biotechnology for Societ
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An Overview Biotechnology is a set
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In a project to identify, map and t
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Medicinal and Aromatic Plants Four
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wax related gene fragments having h
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esearch; appropriate regulatory mec
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Science or Food Science & Technolog
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and the National Research Centre Ca
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many sophisticated instrumentation
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Advisory Structure Standing Advisor
Page 26 and 27: Human Resource Development The Depa
Page 28 and 29: a rapidly advancing area and teache
Page 30 and 31: per year for a period of three year
Page 32 and 33: this fellowship and 9 students have
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Page 37 and 38: throughput molecular marker service
Page 39 and 40: plasmamembrane localisation of GFP-
Page 41 and 42: antibiotic biosynthesis in Streptom
Page 43 and 44: the reporting period. Out of these,
Page 45 and 46: in progress. Besides multiplication
Page 47 and 48: primers (Operon) under the series O
Page 49 and 50: a total of 326 cotyledonary node ex
Page 51 and 52: Magnaportha grisea, showed signific
Page 53 and 54: some diseases are the two most impo
Page 55 and 56: For utilization of Multiple Aleuron
Page 57 and 58: BGA transformations, MPS transforma
Page 59 and 60: development of root knot nematode,
Page 61 and 62: plant growth and development. Due t
Page 63 and 64: significant weed control at seedlin
Page 65 and 66: three rice accessions with enhanced
Page 67 and 68: oth adults and nymphs. First year m
Page 69 and 70: c-DNA probes were developed using a
Page 71 and 72: across all the 462 clones with allo
Page 73 and 74: R & D programme have also been supp
Page 75: Inauguration of Butterfly Park by S
Page 79 and 80: patchouli were found to be similar
Page 81 and 82: With a view to isolate the taxol bi
Page 83 and 84: attributed to the ribosome inactiva
Page 85 and 86: (RBSS) assay. Study was supported a
Page 87 and 88: showed sign of spoilage even after
Page 89 and 90: ody hepatitis (IBH) in domestic fow
Page 91 and 92: FSH, IGF-1 and cumulus granulosa mo
Page 93 and 94: uffalo UTMP cDNA exhibited 94.5, 88
Page 95 and 96: Biosurfactant Work on screening of
Page 97 and 98: conotoxins along with the posttrans
Page 99 and 100: Oligonucleotide probe for monitorin
Page 101 and 102: similarity with the already reporte
Page 103 and 104: studies on expressed sequences of t
Page 105 and 106: factors. Scientists at Sree Chitra
Page 107 and 108: A study was implemented at NCCS, Pu
Page 109 and 110: Medical Biotechnology Health relate
Page 111 and 112: F S C # C e lls 1000 800 600 400 20
Page 113 and 114: the pathogenesis of JE. It has been
Page 115 and 116: Delhi. The protocol for 4-colour wh
Page 117 and 118: Cross Sectional Morphology of the T
Page 119 and 120: Virus Research Centers or Networks
Page 121 and 122: As the clinical trials ae planned t
Page 123 and 124: een implemented for both basic and
Page 125 and 126: low cost for rapid diagnosis of dis
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Under the multi-centric project on
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The genetic basis of Type2 diabetes
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Floor model RBC Reactor - Close-up
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analyzed for the selection of best
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yield of hydrogen in the first stag
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Operational guidelines for collecti
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Programmes for SC and ST Population
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section in the Western Dun Valley t
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aromatic grasses. These women are g
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Poultry and Livestock Farming In an
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conducted on mushroom cultivation,
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probiotic selected strains of Lacto
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hydratase activity was shown by eig
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At Birla Institute for Scientific R
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study at Osmania University and IIC
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DBT Annual Report 2006-07 152
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In the area of recombinant pharma s
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MEC met five times during the year
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Biotechnology Information System Ne
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Bioinformatics National Certificati
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Meetings 1) International Conferenc
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Biotechnology Parks and Incubators
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International Collaboration Interna
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elow: Contraceptive and Reproductiv
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USP method. These included 59 smear
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Danforth); others (IHBT & possibly
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Memorandum of Agreement between Dep
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cytokine-mediated regulation of int
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In stem cell research, the studies
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two pathogens in the acidic environ
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software developed by TCS with supp
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eleased from activated microglia ar
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Genetic linkage mapping in Catharan
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three of the BACs (374Kb) of chromo
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ATPase mRNA binding protein, cathep
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Public Sector Undertaking Bharat Im
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complete protection against a paras
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Administration and Finance Administ
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2. As the Department wants the comm
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GENDER BUDGETING CELL The Departmen
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6. Prof. K. Dharmalingam Member Hea
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19. Prof. Mahtab S. Bamji Member Em
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INTER-DISCIPLINARY RESEARCH COMMITT
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MONITORING-CUM-EVALUATION COMMITTEE
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Dr. Debi P. Sarkar, Professor & Hea
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TASK FORCE ON BIOTECHNOLOGY BASED P
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TASK FORCE ON AGRICULTURAL BIOTECHN
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Prof. H. Sharat Chandra, Director,
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NATIONAL BIORESOURCE DEVELOPMENT BO
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Scientist 'F' Forest Research Insti
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Prof. B. N. Johri Department of Bio
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Annexure-III DBT SPONSORED UNIVERSI
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16. University of Jammu, 10 JNU-CET
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32. Visva-Bharati, 10 JNU-CET Prof
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46. Banaras Hindu 12 JNU-CET Prof.
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LONG TERM 237 Annexure-IV BIOTECHNO
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239 Annexures
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241 Annexures
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243 Annexures
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245 Annexures
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SOFTWARE AVAILABLE WITH BTISNet CEN
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249 Annexures
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12. Jagadish N, Rana R, Mishra D, G
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42. Vats D, Vishwakarma RA, Bhattac
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15. Singh, S., Upadhyay, A.K., Ajay
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PATENTS FILED / SEALED Dr. V. P. Ka
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22. Johny S, Kanginakudru S, Murali
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11. Mallappa, C., Yadav, V., Negi,
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Annexure-X STATEMENT OF BUDGET ESTI
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GST H2O2 HAU HCH hck HCMI HDAC1 HIC
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SATB1 SDL SFC SGOT SGPGI SGPT SH Si
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ANNUAL REPORT - Department of Biotechnology

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