ANNUAL REPORT - Department of Biotechnology

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200 isolates obtained from different parts of the world including Asia, Australia and South America. Using bacteriophages having a broad spectrum of activity, technology for bacteriophage therapy of luminous bacterial disease in shrimp larvae has been developed. In hatchery trials, bacteriophage therapy led to over 80% survival in larvae while antibiotic therapy led to 40% and untreated control showed less than 20% survival . Electron microscopic images of lytic bacteriophages (Phages A-c and E-F belong to siphoviridae and phage D belonds to myoviridae). Hatchery trial of bacteriophage therapy- survival of Penaeus monodon larvae infected with luminous bacteria under various conditions 91 Research and Development
Oligonucleotide probe for monitoring vibrio counts in hatcheries Vibrio sp including luminous V. harveyi, V. vulnificus and V. parahaemolyticus are major bacterial pathogens of shrimp larvae. Monitoring Vibrio counts in hatchery water and larval samples would be an important aspect in health management. To overcome the problem and get a realistic estimate of Vibrio counts, an oligonucleotide probe binding to a region of gyrB gene of all Vibrio spp has been designed. When labeled with alkaline phosphatase, this label could be used for enumeration of Vibrio spp in hatchery environments by colony hybridization technique. Colonies of Vibrio spp hybridizing with alkaline phosphatase labeled oilgonucleotide probe. Genetic characterization of marine organisms Studies on genotypic characterization of antagonistic and detritus degrading bacteria based on their 16S rDNA sequence ere carried out and partial sequences of 6 organisms were deposited in DBT Annual Report 2006-07 92 Genbank. Molecular characterization of antimicrobial peptides in shrimp and their enhanced production was undertaken, which showed broad antimicrobial activity. Studies on novel drugs targeted at Vibrios from marine microorganisms were undertaken and an antivibrio compound produced by Pseudomonas was identified as pyocyanin. A bioprocess technology for large-scale production of marine yeast Candida sake as single cell protein was programmed and executed. An isolate was identified as the source of alkaline protease for industrial applications. The crude enzyme was partially purified and found to have blood stain removal property from fabrics in the absence of any detergent. A training programme in Marine Biotechnology was initiated. Genetic marker for population of cultured animals would be important for genetic selection of populations with commercially important traits like fast growth and disease resistance. Using microsatellite enrichment techniques, two DNA microsatellites were identified in genome of freshwater prawn, Macrobrachium rosenberii . The sequences of these microsatellites, (CA) repeats, 13 (GA) and (GA) repeats have been deposited in 38 30 GenBank with accession number DQ793216 and DQ 793215. Primers binding to flanking region of these microsatellites were designed and evaluated in population of M. rosenbergii from Kerala and Karnataka. The discriminative ability of microsatellite was very good with heterogeneity index of 0.27 ± 0.32 and 0.92 ± 0.29 respectively. Shannon index for these microsatellites were 0.6161 ± 0.2310 and 0.6619 ± 0.2276 respectively. Organ Development Aspects of in vitro organ development and differentiation were undertaken through regeneration and stem cell biology to search for novel biomolecules from hitherto untapped sources that will be useful in the differentiation and regeneration of vertebrate organs. Preliminary studies showed that perivitelline fluid (PVF) of Indian Horse Shoe Crab influences early vertebrate development. Chick embryo explants cultured in vitro were used as a
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ANNUAL REPORT 2006 - 2007 Departmen
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CONTENTS Chapter-1: An Overview 1 A
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Chapter-6: Biotechnology for Societ
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An Overview Biotechnology is a set
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In a project to identify, map and t
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Medicinal and Aromatic Plants Four
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wax related gene fragments having h
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esearch; appropriate regulatory mec
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Science or Food Science & Technolog
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and the National Research Centre Ca
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many sophisticated instrumentation
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Advisory Structure Standing Advisor
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Human Resource Development The Depa
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a rapidly advancing area and teache
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per year for a period of three year
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this fellowship and 9 students have
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80 70 60 50 40 30 20 10 0 1600 1400
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throughput molecular marker service
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plasmamembrane localisation of GFP-
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antibiotic biosynthesis in Streptom
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the reporting period. Out of these,
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in progress. Besides multiplication
Page 47 and 48: primers (Operon) under the series O
Page 49 and 50: a total of 326 cotyledonary node ex
Page 51 and 52: Magnaportha grisea, showed signific
Page 53 and 54: some diseases are the two most impo
Page 55 and 56: For utilization of Multiple Aleuron
Page 57 and 58: BGA transformations, MPS transforma
Page 59 and 60: development of root knot nematode,
Page 61 and 62: plant growth and development. Due t
Page 63 and 64: significant weed control at seedlin
Page 65 and 66: three rice accessions with enhanced
Page 67 and 68: oth adults and nymphs. First year m
Page 69 and 70: c-DNA probes were developed using a
Page 71 and 72: across all the 462 clones with allo
Page 73 and 74: R & D programme have also been supp
Page 75 and 76: Inauguration of Butterfly Park by S
Page 77 and 78: Germlasing of NAPs conversed in gen
Page 79 and 80: patchouli were found to be similar
Page 81 and 82: With a view to isolate the taxol bi
Page 83 and 84: attributed to the ribosome inactiva
Page 85 and 86: (RBSS) assay. Study was supported a
Page 87 and 88: showed sign of spoilage even after
Page 89 and 90: ody hepatitis (IBH) in domestic fow
Page 91 and 92: FSH, IGF-1 and cumulus granulosa mo
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Page 95 and 96: Biosurfactant Work on screening of
Page 97: conotoxins along with the posttrans
Page 101 and 102: similarity with the already reporte
Page 103 and 104: studies on expressed sequences of t
Page 105 and 106: factors. Scientists at Sree Chitra
Page 107 and 108: A study was implemented at NCCS, Pu
Page 109 and 110: Medical Biotechnology Health relate
Page 111 and 112: F S C # C e lls 1000 800 600 400 20
Page 113 and 114: the pathogenesis of JE. It has been
Page 115 and 116: Delhi. The protocol for 4-colour wh
Page 117 and 118: Cross Sectional Morphology of the T
Page 119 and 120: Virus Research Centers or Networks
Page 121 and 122: As the clinical trials ae planned t
Page 123 and 124: een implemented for both basic and
Page 125 and 126: low cost for rapid diagnosis of dis
Page 127 and 128: Under the multi-centric project on
Page 129 and 130: The genetic basis of Type2 diabetes
Page 131 and 132: Floor model RBC Reactor - Close-up
Page 133 and 134: analyzed for the selection of best
Page 135 and 136: yield of hydrogen in the first stag
Page 137 and 138: Operational guidelines for collecti
Page 140 and 141: Programmes for SC and ST Population
Page 142 and 143: section in the Western Dun Valley t
Page 144 and 145: aromatic grasses. These women are g
Page 146 and 147: Poultry and Livestock Farming In an
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conducted on mushroom cultivation,
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probiotic selected strains of Lacto
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hydratase activity was shown by eig
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At Birla Institute for Scientific R
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study at Osmania University and IIC
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DBT Annual Report 2006-07 152
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In the area of recombinant pharma s
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MEC met five times during the year
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Biotechnology Information System Ne
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Bioinformatics National Certificati
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Meetings 1) International Conferenc
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Biotechnology Parks and Incubators
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International Collaboration Interna
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elow: Contraceptive and Reproductiv
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USP method. These included 59 smear
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Danforth); others (IHBT & possibly
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Memorandum of Agreement between Dep
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cytokine-mediated regulation of int
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In stem cell research, the studies
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two pathogens in the acidic environ
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software developed by TCS with supp
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eleased from activated microglia ar
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Genetic linkage mapping in Catharan
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three of the BACs (374Kb) of chromo
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ATPase mRNA binding protein, cathep
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Public Sector Undertaking Bharat Im
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complete protection against a paras
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Administration and Finance Administ
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2. As the Department wants the comm
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GENDER BUDGETING CELL The Departmen
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6. Prof. K. Dharmalingam Member Hea
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19. Prof. Mahtab S. Bamji Member Em
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INTER-DISCIPLINARY RESEARCH COMMITT
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MONITORING-CUM-EVALUATION COMMITTEE
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Dr. Debi P. Sarkar, Professor & Hea
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TASK FORCE ON BIOTECHNOLOGY BASED P
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TASK FORCE ON AGRICULTURAL BIOTECHN
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Prof. H. Sharat Chandra, Director,
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NATIONAL BIORESOURCE DEVELOPMENT BO
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Scientist 'F' Forest Research Insti
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Prof. B. N. Johri Department of Bio
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Annexure-III DBT SPONSORED UNIVERSI
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16. University of Jammu, 10 JNU-CET
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32. Visva-Bharati, 10 JNU-CET Prof
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46. Banaras Hindu 12 JNU-CET Prof.
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LONG TERM 237 Annexure-IV BIOTECHNO
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239 Annexures
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241 Annexures
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243 Annexures
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245 Annexures
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SOFTWARE AVAILABLE WITH BTISNet CEN
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249 Annexures
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12. Jagadish N, Rana R, Mishra D, G
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42. Vats D, Vishwakarma RA, Bhattac
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15. Singh, S., Upadhyay, A.K., Ajay
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PATENTS FILED / SEALED Dr. V. P. Ka
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22. Johny S, Kanginakudru S, Murali
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11. Mallappa, C., Yadav, V., Negi,
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Annexure-X STATEMENT OF BUDGET ESTI
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GST H2O2 HAU HCH hck HCMI HDAC1 HIC
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SATB1 SDL SFC SGOT SGPGI SGPT SH Si
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ANNUAL REPORT - Department of Biotechnology

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