ischaemic preconditioning of the human heart. - Leicester Research ...

It has been reported that K.. %-I-p channels are also participating in the protection of
preconditioning in man [282,3071, however, to the best of my knowledge, there is no
evidence as yet that mitochondrial K:
%-I-p channels are involved in human preconditioning.
The aim of the present chapter was to investigate whether the protection induced by
preconditioning in the human myocardium is mediated by mitochondrial K%1-1,
channels.
To achieve this, I used our model of simulated ischaemia with right atrial trabeculae
obtained from patients undergoing elective cardiac surgery and investigated the effects of
the mitoK,, x, rp channel opener diazoxide, the blockers 5-hydroxydecanoate(5-HD) and
glibenclamide, and the sarcolernmal KA-I-p blocker HMR 1883.
4.2 MATERIALS AND METHODS:
4.2.1 Experimental Preparation
Experiments were performed on trabeculae as described in section 2.3 (Chapter 2).
4.2.2 Solulionsanddnigs
Solutions were prepared daily as described in section 2.3 (Chapter 2). The K.. %-i i, channel
blockers glibenclamide and HMR 1883 were made up to a concentration of 10 pmol in
100 mL K-H solution and sodium 5-hydroxy decanoic acid (5-1-113), was made up to a
concentration of I mmol in 500 m1l. K-H solution. The K. x-i-P openers pinacidil and
diazoxide, were dissolved in DIVISO immediately before being added into experimental
solutions. The final concentration of DMSO was