ischaemic preconditioning of the human heart. - Leicester Research ...

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13 14 15 16 17 18 19 20 21 22 23 24 25 Li, Y. and Kloner, R. A. (1995) Does protein kinase C play a role in ischaemic preconditioning in rat hearts ? Am. J. Physiol. 268, H426-H431 Schulz, R., Rose, J. and Heusch, G. (1994) Involvement of activation of ATP-depenclent potassium channels in ischaemic preconditioning in swine. Am. J. Physiol. 267, H1341-HI352 Walker, D. M., Marber, M. S., Walker, J. M. and Yellon, D. M. (1994) Preconditioning in isolated superfused rabbit papillary muscles. Am. J. Physiol. 266, H1 534-Hl 540 Yellon, D. M., Alkhulaifi, A. M. and Pugsley, W. B. (1993) Preconditioning the human myocardium. Lancet 342, 276-277 Zhang, J. G. and Lindup, W. E. (1993) Role of mitochondria in cisplatin-induced oxidative damage exhibited by rat renal cortical slices. Biochem. Pharmacol. 47,1127-1135 Smolenski, R. T., Lachno, D. R., Ledingliam, S. J. M. and Yacoub, M. H. (1990) Determination of sixteen nucleotides, nucleosides and bases using high-ýerformance liquid chromatography and its application to te study of purine metabolism in hearts for transplantation. J. Chromatogr. 527,414-420 Miyawaki, H., Zhou, X. and Ashraf, M. (1996) Calcium preconditioning elicits strong protection against ischernic injury via a protein kinase C signalling pathway. Circ. Res. 79,137-146 Ashraf, M., Suleiman, J. and Ahmad, M. (1994) Cal' preconditioning elicits a unique protection against the Ca` paradox injury in rat heart, role of adenosine. Circ. Res. 74,360-367 DeLeo, F. R., Renee, J., McCormick, S., Nakamura, M., Apicella, M., Weiss, J. P. and Nauseef, F. (1998) Neutrophils exposed to bacterial lipopolysaccharide upregulate NADPH oxidase assembly. J. Clin. Invest. 101, 455-463 Galifianes, M. and Hearse, D. J. (1990) Species differences in susceptibility to ischaernic injury and responsiveness to myocardial protection. Cardioscience 2,127-143 Hearse, D. J., Braimbridge, M. V. and Jynge, P. (1981) Protection of the Ischaemic Myocardium: Cardioplegia, Raven Press, New York Hearse, D. J. (1980) Release of enzymes from ischaernic myocardium: Degradative problems in heart and skeletal muscle (Wildenthal, K., ed. ), pp. 419-456, Elsevier, Amsterdam Michell, R. H. and Coleman, R. (1979) Structure and permeability of normal and damaged membranes. Enzymes in Cardiology: Diagnosis and Research (Hearse, D. J. and De Leivis, J., eds. ), pp. 59-79, John Wiley, Chichester Myocardial ischaemia/reperfusion in human right atrial slices 26 Prasad, K. and Callaghan, J. C. (1969) Effect of replacement of potassium by rubidium on the transmembrane action potential and contractility of human pý illary muscle. Circ. Res. 24,157-166 27 Gnsh, S., Standen, N. B. and Galifianes, M. (2000) Preconditioning the human myocardium with ischaemia. Studies on the early and delayed protection. Cardiovasc. Res. 45,350-359 28 Buxton, D. B., Mody, F. V., Krivokapich, J. and Schelbert, H. R. (1993) Metabolism in non-ischaemic myocardium during coronary artery occlusion and reperfusion. J. Mol. Cell . Cardiol. 25,667-681 29 Dean, E. N., Shlafer, M. and Nicklas, J. M. (1990) The oxygen consumption paradox of 'stunned myocardium' in dogs. Basic Res. Cardiol. 85,120-131 30 Kawashima, S., Satani, A., Tsumoto, S., Kondo, T., Ikeoka, K., Motrita, M. and Iwasaki, T. (1991) Coronary pressure- flow, pressure-function, and function-myocardial oxygen consumption relations in postischaemic myocardium. Cardiovasc. Res. 25,837-843 31 Laxson, D. D., Homans, D. D., Dai, X. Z., Sublett, E. and Bache, R. J. (1989) Oxygen consumption and coronary reactivity in postischaernic myocardium. Circ. Res. 64, 9-20 32 Gottlieb, R. A., Burleson, K. 0., Kloner, R. A., Babior, B. M. and Engler, R. L. (1994) Reperfusion injury induces apoptosis in rabbit cardiomyocytes. J. Clin. Invest. 94, 1621-1628 33 Maulik, N., Yoshida, T. and Das, D. K. (1998) Oxidative stress developed during the reperfusion of the ischaernic myocardium induces apoptosis. Free Radicals Biol. Med. 24,869-875 34 Piot, C. A., Padmanaban, D., Ursell, P. C., Sievers, R. E. and Wolfe, C. L. (1997) Ischaemic preconditioning decreases apoptosis in rat hearts in vivo. Circulation 96, 1598-1604 35 Budingcr, G. R. S., Chandel, N., Shao, Z. H., Li, C. Q., Melmed, A., Becker, L. M. and Schumacker, P. T. (1996) Cellular energy utilisation and supply dU i hypoxia in embryonic cardiac myocytes. Am. J. Physio 14, L37-1,53 36 Tumiati, L. C., Li, R. K., Weisel, R. D., illiams, W. G. and Mickle, D. A. G. (1994) An in vitro model to stud myocardial ischaemic injury. J. Tissue Culture MethoYs 16,1-9 37 Amos, G. J., Wettwer, E., Metsger, F., Li, Q. and Himmel, H. M. (1996) Differences between outward currents of human atrial and subepicardial ventricular myocytes. J. Physiol. 491,31-50 38 Heidbuchel, H., Vereecke, J. and Carmeliet, E. (1990) Three different potassium channels in human atrium: contribution to the basal potassium conductance. Circ. Res. 66,1277-1286 Recolved S January 2000/4 Aprfl 2000; scAmpted I$ July 2000 C 2000 The Biochemical Society and the Medical Research Society 453
ELSEVIER Abstract Cardiovascular Research 45 (2000) 934-940 Cardiovascular Research www. elsevier. com/locate/cardiores www. elsevier. ni/locate/cardiores Evidence for mitochondrial KATP channels as effectors of human myocardial preconditioning b Sudip Ghosh', Nicholas B. Standen , Manuel Galifianes" 'Division of Cardiac Surgery, Department of Surgery, University of Leicester, Glenfield Hospital, Leicester LE3 9QP UK ýDeparftnenl of Cell Physiology and Pharmacology, University of Leicester, Glenfield Hospital, Leicester LE3 9QP. UK Received 22 September 1999; accepted 10 November 1999 Background: Sublethal periods of ischernia preceding a prolonged interval of ischaemia protect the myocardium. This myocardial preconditioning (PC) appears to be effected by K,,,, channels. These channels occur both in the sarcolemma and the mitochondrial membrane. We investigated whether mitochondrial K,., p channels are the end-effector of PC in the human myocardium. Methods: Right atrium specimens obtained from patients undergoing cardiac surgery were prepared and incubated in buffer solution at 37*C. After 30-min stabilisation, the muscles were made ischemic for 90 min and then reperftised for 120 min. The preparations were randomised into eight experimental groups (n=6/group): (1) Aerobic control - incubated in oxygenated buffer for 210 min, (2) ischemia alone - 90 min ischemia followed by 120 min reperfusion, (3) PC - preconditioned with 5 min ischemia/5 min reperfusion, (4) Glibenclamide (10 ýLlvl) in the incubation media for 10 min before PC, (5) 5-hydroxydecanoate (5-HD, MitoK,,,, blocker, I mM) in the incubation media for 10 min before PC, (6) HMR 1883 (SarcKAT, blocker, 10 ýLM) in the incubation media for 10 min before PC, (7) Pinacidil (0.5 mM) in the incubation media for 10 min before ischemia, and (8) Diazoxide (MitoKA,,, opener, 0.1 mM) in the incubation media for 10 min before ischemia. Creatinine kinase leakage into the medium (CK, IU/g wet wt) and MTT reduction (OD/mg wet wt. ), an index of cell viability, were assessed at the end of the experiment. Results: Ischernia alone resulted in a significant increase in CK leakage (8.01±0.35) and decrease in MTT (0.15±0.01) from the values seen in the aerobic control (2.24±0.52 and 0.78±0.10 respectively, P
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ISCHAEMIC PRECONDITIONING OF THE HU
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ABSTRACT Ischaemic preconditioning
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andomised clinical study with the f
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CHAPTERI Introduction 35 Matefials
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Discussion 151 CHAPTER Vill Conclus
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44 CK leakage and MTT reduction on
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The Wellcome Trust and The British
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Presentations International "The Ef
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Abbreviations An attempt has been m
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1.1 Introduction Ischaernic heart d
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1.2 Myocardial Ischaemia Myocardial
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hours up to several days to complet
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phosphates and its influence on hyd
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1.4 Myocardial Protection This refe
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performed. This process is repeated
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1.6 Possible Mechanisms of Ischaemi
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kilodalton stress proteins. These p
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and these proteins have been sugges
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K vi-1, channels were initially fou
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tissues the K.. %,.,, channels are
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Subsequently, Jaburek et al 1145] i
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venous capacitance vessels. At ther
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preconditioning to be investigated
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In the angioplasty model, Tomai et
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patients were randomized into two g
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CHAPTER 11 THE HUMAN RIGHT ATRIAL T
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inexpensive. Briefly in this model,
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of each ischemic period, slices wer
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30 nini FO linlin L 60 min 120 inin
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In Study 3 (see Figure 2.4), slices
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D. Metabolite analysis Samples were
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leakage is calculated as the net LD
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B. Effect of the severity of ischae
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2.11 DISCUSSION: The present studie
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clearly insufficient to induce myoc
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The present model may facilitate th
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2.12 Conclusiow I have characterise
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3.5 3 V 2.5 3: m Je m Z0 z 2 1.5 0.
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(3 3 2.5 a 1.5 - -r T 0 2 0.5 0 I P
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Q oro . 21 . 0 'a IP 10 '. R -C3 A
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=3 0 h X CD 0 x CD :3 0 , 7, CD 0 0
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CHAPTER III PRECONDITIONING THE HUM
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3.2 MATERIALS AND METHODS-. 3.2.1 E
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A117'reduction- At the end of the e
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-4 ýi r. () .1 a 0 1 tli n F5* - C
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v M. %; n C) .0 ý U ý ý - - - -2
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leakage resulting from precondition
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3.5 DISCUSSION: The present studies
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during cardiac surgery totalling 10
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different. The cellular mechanisms
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cn Je 8 7 6 5 4 1 0 0.8 0.7 0.6 0.5
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8 6 C) 2 0.7 0.6 Q) 0.5 0) E 25 0.4
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6 5 CY) ,u3 CF) m -ýe m 2 1 0 -0.8
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CHAPTERIV THE ROLE OF THE KATPCHANN
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It has been reported that K.. %-I-p
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0 a- I a 3 I cr =r Cl. 2 qQ 0'-ý 0
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Figure 4.7 shows that ischaemia alo
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Figure 4.2 Dosc-responsc experiment
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Figure 4.4 Dose-response experiment
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Figure 4.6 Dosc-response experiment
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1 0.9 -ý 0.8 0.7 E 0.6 0.5 0.4 0.2
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activity, and showed that diazoxide
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Furthermore, Van den Hoek et al [3
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mechanism by which the opening of m
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5.1 INTRODUCTION: Within the enormo
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5.2 MATERIALS AND METHODS: 5.2.1 Ex
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M77'rechiction: At the end of the e
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oc 0 0 cr F; * 10 eb I ý; o I 5 0
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Pages missing original in the
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esulted in a decrease of CK leakage
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are on long-term hypoglycaernics or
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absence of uniformity of expeniment
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5.7 Conclusion Preconditioning is a
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0.9 0.8 07 m 0.6 E -0 0t 4 0.3 02 0
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-0 1.2 1 &8 0.6 04 0.2 o Aerobic Is
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6.1 INTRODUCIFION' Life expectancv
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(MI-T) to blue t'()rmazan product C
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The demonstration that ischaemic in
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Table 61 Patients' demographic and
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Nure 6,1: Creatine Kinase (CK) leak
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CHAPTER VII IN VIVO STUDIES ON T"E
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The apparent discrepancy bet,.,,, e
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In the off-pump group, coronary byp
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eduction as described in section 3.
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7.4.2 In vitro studies Figures 7.3A
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preconditioning stimulus in man is
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myocardium. Several investigators [
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Table 7.2 - Patients haemod-v-namic
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(0 -I 3 CD CD CD 1 3 0 :3 0 0 =r w
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0 co t X 3 co 0 0 Cumulative Releas
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-& a) c) UT 0 =r w CD 3 CD 0 1ý (a
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When ischaernic preconditioning was
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evidence tbr the involvement ot'PKC
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institution o fcardiopu Imo nary by
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Biblioqraphv 1. Abete P, Ferrara N,
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14. Auchampach J, Cavero, I and Gro
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27. Becker LB, Van den Hoek TLV, Sh
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41. Bums PG, Krunkenkamp IB, Calder
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55. Cohen G, Shirai T, Weisel RD, e
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69. Currie RW and White FP (1983).
Page 201 and 202: 83. Downey JM, Liu GS and Thornton
Page 203 and 204: 97. Ganote C, Armstrong S and Downe
Page 205 and 206: 110. Grover GJ, D'Alonzo A, Hess T,
Page 207 and 208: 122. Hamilton T and Weston A (1989)
Page 209 and 210: 137. Hu H, Sato T, Seharaseyon J, L
Page 211 and 212: 151. Jennings R, Steenbergen C, Kin
Page 213 and 214: 164. Kelpzig H, Kobert G, Mafter C,
Page 215 and 216: 178. Lamping K, Christensen C, Pelc
Page 217 and 218: 192. Liu GS, Thornton J, Van Winkle
Page 219 and 220: 206. Maulik N, Yoshida T, Das DK. (
Page 221 and 222: 219. Miyawaki H, Zhou X, Ashraf, M.
Page 223 and 224: 233. Okazaki Y, Kodama K, Sato H, K
Page 225 and 226: 248. Pryzlenk K, Li G, Whittaker P
Page 227 and 228: 260. Saito S, Tamura Y, Moriuchi M,
Page 229 and 230: 274. Schulz R, Post H, Valhaus C, e
Page 231 and 232: 288. Stewart JR, Blackwell WH, Crut
Page 233 and 234: 300. Tani M, Suganuma Y, Hasegawa H
Page 235 and 236: 312. Tosaki A, Engelman DT, Engelma
Page 237 and 238: 324. Walker D, Marber M, Walker J a
Page 239 and 240: 337. Yamashita N, Nishida M, Hoshid
Page 241 and 242: 351. Zhang JG, Lindup WE. (1993). R
Page 243 and 244: 444 J. -G. Zhang and others are dif
Page 245 and 246: 446 J. -G. Zhang and others Assessm
Page 247 and 248: 448 J. -G. Zhang and others 0.9 0.8
Page 249 and 250: 450 J. -G. Zhang and others C E 07
Page 251: 4S2 J. -G. Zhang and othen effects
Page 255 and 256: 936 S. Ghosh et al. / Cardiovascula
Page 257 and 258: 938 S. Ghosh et al. / Cardiovascula
Page 259 and 260: 940 S. Ghosh et al. [41 Goto M, Liu
Page 261 and 262: 712 0" et &L h wcomMe geg in Obwa»
Page 263 and 264: 714 Gbosb ot aL hmoo dmoWmbDbsnedH
Page 265 and 266: 716 chmä et aL Pi unamOso äu In O
Page 267: 718 ehe* et &L ditioning in the hum
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