ischaemic preconditioning of the human heart. - Leicester Research ...

In Study 3 (see Figure 2.4), slices (n=6/group) were subjected to a fixed 60 min
period of ischaerma and then randomized to follow various times of reoxygenation
(2,4,12 and 24 hr). An additional study (n=4/group) was performed using the same
experimental protocol in the presence of neutrophils obtained from the same patients
from whom the right atrial appendage was removed. As in previous studies, LDH
leakage and tissue viability were determined at the end of the experimental period.
Group 10 min 2 lir
(Aerobic Control)
Group 2J 30 min 2 lir
(Reoxygenation for 2 hr)
Group 3
(Reoxygenation for 4 hr
7,4 hr
Group 4 12 hr
(Reoxygenation for 12 h
Group 5
(Reoxygenation for 24 h%ullibration
Iscliacillia
Reoxygmation
Figure 2.4. Experimental protocols for Study 3. In Group I the slices were then
incubated aerobically for 180 min. In all the other groups the slices were subjected
to 60 min ischaernia followed by 2,4,12 and 24 hr of reoxygenation.
2.6 Measured End-points
A. Lactate dehydrogenase (LDH) leakageý
The activity of LDH leakage into the media (U/g wet wt) was assayed by a kinetic
ultraviolet method based on the formation of NAD (Sigma Catalogue No. 1340-K).
43
24 lir