ischaemic preconditioning of the human heart. - Leicester Research ...

wet wt/min, p= NS) and 24 hr (876 ± 40 nmol 02/9 wet wt/min) of incubation, but
again by 48 hr oxygen consumption dramatically decreased to 20% of the initial
values (184 1 12 nmol 02/g wet wt/min, p-, 0.05). These findings further support
that this human atfium preparation is viable and stable for at least 24 hr of
incubation but not for 48 hr.
li. vue metabohles atid mjmardud etier*, stalus: Table 2.1 shows the high-energy
phosphate content of the slices following the various time periods of aerobic
incubation. The ATP, ADP and AMP contents, ADP/ATP ratio and the total
adenylate pool were all similar in the slices aerobically incubated for 0.5,4 and 24
hr. However, in line with previous results there was a significant reduction in all
metabolites after 48 hr of aerobic incubation.
Morphological Assessment
The results of the semi-quantative morphological examination are shown in Figures
2.9. Figures 2.1 Oa, b and c show representative high resolution electron transmission
micrographs of grade I (normal), grade 2 (mild damage) and grade 3 (severe
damage). The morphological appearance of specimens aerobically perfused for 0.5,
4 and 24 hr was similar, however, significant damage was observed in specimens
aerobically perfused for 48 hr.
48