Page 2 Plant-Bacteria Interactions Edited by Iqbal Ahmad, John ...

140j 7 Quorum Sensing in Bacteria: Potential in Plant Health Protection
Table 7.2 Bioassay test strain used for the detection of
autoinducer (AHL) and QS inhibition.
Reporter strain Description AHLs detected References
E. coli VJS533 (pHV2001) V. fischeri ES114 lux regulon
with inactivated LuxI in
3-Oxo hexanoyl-HSL, hexanoyl-HSL,
3-oxooctanoyl-
pBR322; Ap R
HSL, octanoyl-HSL
A. tumefaciens NTI traI::lacZ and traR on sep-
(pJM749, pSVB33-23) arate plasmids; pTi is
cured; Cb R ,Km R
3-Oxo hexanoyl-HSLs, oc- [130]
tanoyl-HSL and other acyl-
HSLs
V. harveyi D1 Unknown mutation result- 3-Hydroxy, butanoyl-HSL, [128]
ing in reduced autoinducer
production
3-hydroxyl valeryl-HSL
E. coli MG-4 (pKDT17) LasB::lacZ translational fusion
and ptac-rhlR; Ap R
2-Hydroxy,3-oxo and un- [78,129]
substituted acyl-HSLs with
side chain lengths of 8–14
carbons
C. violaceum CV0 blu cviI::Tn5 xylE (inactivated
cviI, an autoinducer
synthase required for violacein
production): Hg R ,
Km R ,Cm R
Hexanoyl-HSL, butanoyl- [59]
HSL, 3-oxohexanoyl-HSL,
octanoyl-HSL, acyl-HSLs
with longer side chains can
be detected by screening
for inhibition of hexanoyl-
HSL-mediated violacein
production
E. coli XL1 Blue (pECp61.5) rhlA::lacZ translation fusion
and ptac-rhlR in
pSW205; Ap R
Butanoyl-HSL, hexanoyl- [129]
HSL
R. solanacearum (p395B) Inactivated solI, p395B
contains aidA::lacZ fusion,
Nx R ,Sp R ,Tc R
[117]
A. tumefaciens A136 traI–lacZ fusion (pCF218)
(pCF372), AHL biosensor
[24]
A. tumefaciens KYC6 Positive control for AHL 3-oxo-C8-HSL
[24]
assay
overproducer
C. violaceum ATCC 12472 Type strain (QSI indicator
strain)
ATCC
C. violaceum O26 MiniTn5 mutant of 31532,
AHL biosensor
[26]
C. violaceum ATCC 31532 Nonpigmented, positive
control for AHL assay
C6-HSL production ATCC
P. aeruginosa PAO1 Positive control for QSI C4-HSL and 3-oxo-C12-HSL
production
V Deretic
P. aeruginosa PDO 100 RhlI mutant C4-HSL [132]
P. aeruginosa 30–84 RhlI, lasI mutant C4-HSL HSL
and 3-oxo-C12- [132]
P. aeruginosa PAO-MW1 Phenazine production, QSI
indicator and type strain
[133]
Environmental bacterial QSI screening, Spring
[134]
isolates
Lake, San Marcos, TX
Partially adapted from McCLean et al. [134]. Sources: ATCC, American Type Culture Collection;
V. Deretic, Department Molecular Genetics and Microbiology, University of New Mexico Health
Sciences Center, Albuquerque, NM; E.P. Greenberg, Department of Microbiology, University of
Iowa, Iowa City, IA.
[129]