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Page 2 Plant-Bacteria Interactions Edited by Iqbal Ahmad, John ...

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24j 2 Physicochemical Approaches to Studying <strong>Plant</strong> Growth Promoting Rhizobacteria<br />

Figure 2.2 Infrared spectra of dried biomass of A. brasilense<br />

(facultatively endophytic strain Sp245) grown (a) in a standard<br />

phosphate–malate medium (control) and in the same medium in<br />

the presence of 0.2 mM Co 2þ (b), Cu 2þ (c) or Zn 2þ (d) [10].<br />

of strain Sp7 to outcompete other co-inoculated strains [27], as well as to a lower<br />

sensitivity of strain Sp7, as compared to strain Sp245, to copper ions, including a less<br />

pronounced copper-induced decrease in auxin production [12,30].<br />

As noted above, the amount of cobalt accumulated <strong>by</strong> strain Sp7 (up to about<br />

0.01% wt/wt dry biomass) induces a significant metabolic response in the bacterium,<br />

comparable to that of about fourfold higher amount of copper or about 36-fold

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