Etude de différentes méthodes de biofonctionnalisation pour la ...

Ill, Etudes du film Lan gmuir-Blodgett enzymatique
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(b)
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40
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Figure 3. Transfer ratio obtained for 8 mixed enzyme/ODA LB 11/rn depositions: (a)
transfer pressure =30 mNm4; (b) transfer pressure =40 mNrn'.
Curiously, it was found that for a dipping pressure of 40 mN m' (figure 3b), only Y type deposition
occurs. Moreover, whatever the dipping pressure, it has been found that a gradual decrease in
downstroke TR occurred.
3.2.2. AFM and FTIR
Only two mixed LB enzyme/ODA monolayers were deposited onto the different substrates, as it
was impossible to exceed this value for surface pressure equal to 30 mN m
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200.0
150.0
i 00.0
50.0
0.0
P4:
A
200.0
150.0
100.0
50.0
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(a)
(b)
Figure 4. Topographic images of two BuChE/ODA LB/i/ms by AFM
(scan area of 5 jim): (a) transfer pressure = 30 mNm ; (b) transfer
pressure = 40 mN m.
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