Etude de différentes méthodes de biofonctionnalisation pour la ...

IV. Réalisation de membranes enzymatiques épaisses pour microcapteurs ENFETs
Type of
ENFETs
Storage
conditions
Results
PVA/SbQ
ENFETs
In dry and dark
state,
+4°C
Storage time(days) 1 12 18 25 29 33 47 56 63 70 77 88 98 108 140 238 266
Sensor response (%) loo 100 100 102 89 83 83 78 83 89 83 90 93 90 83 83 78
In buffer
solution,+4°C
Storage time (days) 1 2 5 9 14 21 28 42 53 80 115 122 137 168 210
Sensor response (%) 100 103 106 106 95 92 85 69 74 53 23 35 37 37 33
BSA/GA
ENFETs
lndryanddark
state,
+4°C
StoragetJme(days) 1 3 8 15 21 35 42
Sensorresponse(%) loo 100 75 70 75 35 40
In buffer
sorution,+4°C
Storage time (days) 1 3 12 18 22 62
Sensor response (%) 100 93 72 83 84 41
Measurements were carried out with 18 mM butyryicholine chloride as substrate in 1 mM phosphate buffer
(pH=8.0). 100% is given relative to the value of the first day.
Tab. I. Effect of storage conditions on the stability of BuChE-FETs