2009-special b.vp

ðàòîðíûå èññëåäîâàíèÿ ïî çîëüíîñòè ñåìÿí è ëåñíîéïîäñòèëêè ñåâåðíûõ ÷àñòåé òàåæíî-ëåñíûõ ëàíäøàôòîâðåñïóáëèêè Êàðåëèÿ. Àðõàíãåëüñêîé îáëàñòè, ðåñïóáëèêèÊîìè, íàèáîëåå áëèçêèõ ïî ïðèðîäíî-êëèìàòè÷åñêèìóñëîâèÿì ê Êîëüñêîìó ñåâåðó. Ýòèì ìîæíîîïðåäåëèòü ïåðñïåêòèâíûå íàñàæäåíèÿ ýòèõ ðåãèîíîâäëÿ ñáîðà ñåìÿí ñ íèçêîé çîëüíîñòüþ è äàëüíåéøåãî èõèñïîëüçîâàíèÿ â ëåñîêóëüòóðíîì ïðîèçâîäñòâå Êîëüñêîãîïîëóîñòðîâà.Ïîëîæèòåëüíîå ðàçðåøåíèå âñåõ âûøåèçëîæåííûõïðîáëåì áóäåò èìåòü íå òîëüêî ïðèêëàäíîå çíà÷åíèåäëÿ ëåñíîãî õîçÿéñòâà Êîëüñêîãî ïîëóîñòðîâà, íî è ïîçâîëèòãëóáæå ïîíÿòü çàêîíû. Óïðàâëÿþùèå ïîâåäåíèåìè âçàèìîäåéñòâèåì áàçèñíûõ ñòðóêòóð ïðèðîäû Êîëüñêîãîñåâåðà. Äëÿ ýòîãî ïîòðåáóåòñÿ ïðîâåäåíèå ðàçëè÷íîãîðîäà ýêñïåðèìåíòàëüíûõ ðàáîò ñ ñåìåííûììàòåðèàëîì ðàçíîãî ïðîèñõîæäåíèÿ, à òàê æå ñ ïðîáàìèëåñíûõ ïîäñòèëîê â ëåñîðàñòèòåëüíûõ áèîöåíîçàõ Ñåâåðî-çàïàäàè Êîëüñêîãî ïîëóîñòðîâà.1. Äîáðîâîëüñêèé Â. Â. Ãåîãðàôèÿ ïî÷â ñ îñíîâàìè ïî÷âîâåäåíèÿ:Ó÷åáíèê äëÿ âóçîâ — Ì.: Ãóìàíèò. èçä. öåíòð ÂËÀÄÎÑ,1999. 208 ñ.2. Êàðïà÷åâñêèé Ë. Î. Ëåñ è ëåñíûå ïî÷âû. — Ì.: Ëåñíàÿ ïðîìûøëåííîñòü,1981. — Ñ. 76 – 77.3. Íàêâàñèíà Å. Í., Áåäðèöêàÿ Ò. Â. Ñåìåííûå ïëàíòàöèè ñåâåðíûõýêîòèïîâ ñîñíû îáûêíîâåííîé. — Àðõàíãåëüñê, ÈçäàòåëüñòâîÏîìîðñêîãî ãîñóäàðñòâåííîãî óíèâåðñèòåòà, 1999. — ñ.8.4. Íèêîíîâ Â. Â., Ñèçîâ È. È. Îá àäàïòàöèîííûõ ñïîñîáíîñòÿõëèñòâåííèöû (Larix Mill.) íà Êîëüñêîì Ñåâåðå. — Ë.: Íàóêà,1987. — Ñ. 96 – 105.5. Öâåòêîâ Â. Ô. Ñîñíÿêè Êîëüñêîé ëåñîðàñòèòåëüíîé îáëàñòè èâåäåíèå õîçÿéñòâà â íèõ. — Àðõàíãåëüñê, 2002 — ñ.139. 1Botany Department, A. C. College, Jalpaiguri, India, somrita25@gmail.com2Botany Department, North Bengal University, Siliguri, India, bncnbu@gmail.com Colletotrichum gloeosporioides Penz. (Sacc.) [teleomorphGlomerella cingulata (Stoneman) Spauld.&Schrenk.] causes brown blight of tea [Camellia sinensis(L.) O. Kuntze] plant, which is a woody perennial crop. Differentvarieties are expected to vary in tolerance levels to aparticular pathogen. Since proteins are important componentsof any plant, which reflect the way in which the plantreacts to the pathological and other stresses, it was decidedto conduct total soluble protein profile analysis of healthyand pathogen inoculated tea leaves of different varieties.Eighteen tea varieties obtained from Tea GermplasmBank, Botany Department, were initially screened for resistanceagainst G. cingulata following the detached leaf inoculationmethod (Dickens and Cook,1989), percentage lesionproduced being inversely proportional to the resistance.Total soluble proteins of tea leaves of different varietieswere extracted (Chakraborty et al, 1995), estimated (Lowryet al, 1951) and analysed by SDS-PAGE (Sambrook et al,1989) according to the standard procedures.It is evident from the table that in the susceptible varieties(TV-22, T-17, UP-26, CP-1/1, BSS-3) the protein contentdecreased significantly. SDS- PAGE analysis of theseextracted proteins reflected the protein content changes,with decreased content showing reduction in number ofbands. On the other hand, in the resistant varieties (TV-18,TV-30, BSS-2, UP-9, P-312, TV-25 and TV-26) there wasno significant change in total protein content on infection.In these varieties leaf proteins of ca. 97.4, 94.3 kDa weredegraded by the fungus. New proteins were not induced. Insome of these varieties (TV-30, P-312, TV-25 and TV-36)these two proteins were newly synthesized on inoculationwith G. cingulata. Thus, these seem to be associated withresistance. Surprisingly, their initial presence in the host isnot associated with resistance. It is interesting that in somemoderately resistant varieties (TV-29, BSS-2, BS/7A/76and AV-2) the banding pattern did not change at all.Therefore, total protein analysis throws some light onthe particular proteins involved in the resistance towardsbrown blight pathogen. It is also clear that the fungus degradessome proteins of the host, breaking down its resistancemechanism, while the resistant varieties were able to tacklethe stress by maintaining the pool of the total proteins.1. Chakraborty B. N., Basu P., Das R., Saha A. and Chakraborty U.Annals of Applied Biology, 1995. — p.11.2. Dickens J. S. and Cook R. T. A. Plant Pathology, 1989. — p.147.3. Lowry O. H., Rosenbrough N. J., Farr A. L. and Randall R. J. Journalof Biological Chemistry, 1951. — p.265.4. Sambrook J., Fritch E. F. and Maniatis T. — Molecular cloning —a laboratory manual, 2 nd edition, 1989. — p 18.60.Àãðàðíàÿ Ðîññèÿ. 2009. Ñïåöèàëüíûé âûïóñê 131