Echerichia coli kasvu heterogeensus ja toksiin-antitoksiini süsteemid

2. Eksperementaalosa2.1. Materjal ja metoodika2.1.1. Bakteritüved ja plasmiididKatsetes kasutati tabelis 2. kirjeldatud E. coli tüvesid ning tabelis 3. kirjeldatud plasmiide.Tabel 2. Kasutatavad bakteritüved. Tüvedel, mis pärinesid Yale ülikooli E. coli kollektsioonist (CGSC) on viitenamärgitud nende kood antud asutuses.E. coli tüvi genotüüp viideHM22BW25113dapA zde-264: :Tn10 hipA7F-, ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), &lambda-,rph-1, ∆(rhaD-rhaB)568, hsdR514(Moyed jaBertrand, 1983)(Datsenko jaWanner, 2000)BW25113∆relBEBW23473PS266PS265KJ173JS10GM36F-, ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), &lambda-, BW25113 milleltrph-1, ∆(rhaD-rhaB)568, hsdR514, ∆relBEeemaldatud relBE.F-, ∆(argF-lac)169, ∆uidA3::pir+, recA1, rpoS396(Am)?,(Haldimann jaendA9(del-ins)::FRT?, rph-1, hsdR514, rob-1, creC510Wanner, 2001)F-, [araD139]B/r, ∆(argF-lac)169, phoR9999, &lambda-,flhD5301, ∆(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22,ijRK498::Tn5, secE15(Cs) CGSC# 7144F-, [araD139]B/r, ∆(argF-lac)169, phoR9999, &lambda-,flhD5301, ∆(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22,zijRK498::Tn5, secE501(Cs) CGSC# 7143F-, ∆(araA-leu)7697, [araD139]B/r, ∆(codB-lacI)3, phoR82, zaj-2085::Tn10, secD29(Cs), galK16, galE15(GalS), &lambda-, e14-,relA1, rpsL150(strR), spoT1, mcrB1? CGSC# 7145F-, araC14, leuB6(Am), secA206(aziR), fhuA23, proA180,lacZ36, tsx-67, purE42, glnV44(AS), &lambda-, trpE38, lysA23,rpsL109(strR), ftsX2000(Cs), xylA5, mtl-1, metE70, thi-1 CGSC# 6480F-, thr-1, leuB6(Am), fhuA2, pro-33, lacY1, dnaX36(ts),glnV44(AS), gal-6, &lambda-, hisG1(Fs), rfbC1, galP63, xyl-7,mtlA2, ∆argH1, rplL9(L?), thi-1 CGSC# 6861HC123 Hfr(PO2A), fhuA22, ompF627(T2R), relA1, dnaN159(ts), metB1 CGSC# 6844Ax655F-, thr-1, araC14, leuB6(Am), ftsI2158(ts), ∆(gpt-proA)62, lacY1,tsx-33, qsr'-0, glnV44(AS), galK2(Oc), &lambda-, Rac-0,hisG4(Oc), rfbC1, mgl-50?, rpsL31(strR), kdgK51, xylA5, mtl-1,argE3(Oc), thi-1 CGSC# 6888KY1429F-, [araD139]B/r, ∆(argF-lac)169, &lambda-, flhD5301, ∆(fruKyeiR)725(fruA25),relA1, rpsL150(strR), zhh-50::Tn10,rpoH606(ts), rbsR22, ∆(fimB-fimE)632(::IS1), deoC1 CGSC# 6934CM5255E177NH4920F-, lacZ53(Am), &lambda-, thyA36, IN(rrnD-rrnE)1,rpsL151(strR), zig-1863::Tn10, polA4113(ts), deoC3 CGSC# 6546F-, thr-1, leuB6(Am), fhuA21, lacY1, glnV44(AS), &lambda-,rfbC1, thyA6, rpsL67(strR), glpR101?, dnaA177(ts), thi-1, deoC1 CGSC# 5029F-, thr-1, araC14?, leuB6(Am), ∆(gpt-proA)62, lacY1, tsx-33,glnV44(AS)?, galK2(Oc), &lambda-, hisG4(Oc), recA44(ts),rpsL31(strR), xylA5, mtl-1?, argE3(Oc), thi-1, deoB16 CGSC# 504116