3. Umbruch 4.4..2005 - Online Pot

56 R.G. Pertwee
Table 3. Anti-inflammatory effects of CBD in vivo
Bioassay Effect of CBD Dose Reference
Oedema induced in mice by sub-plantar injection of carrageenan Inhibition 100 mg administered 19 h before [80]
carrageenan by abdominal transdermal
patches using ethosomal carriers
Clinical signs of arthritis in mice (swelling, erythema, oedema, and/or Inhibition 5, 10 or 20 mg/kg/day i.p. [77]
rigidity of joints) and histologically assessed joint damage induced by or 25 or 50 mg/kg/day p.o. *
type II collagen in complete Freund’s adjuvant injected intradermally
at the base of the tail
Bovine type II collagen-induced IFN-γ release from lymph node cells Inhibition 5 mg/kg/day i.p. †
[77]
taken from arthritic mice
In vitro TNF release from synovial cells taken from knee joints of Inhibition 5 mg/kg i.p. †
[77]
arthritic mice
LPS-induced elevation of mouse serum TNF Inhibition 10 mg/kg i.p. or s.c. [77]
Ca 2+ ionophore-induced stimulation of LTB4 production in mouse plasma Inhibition (ex vivo) 10 mg/kg p.o. [81]
Ca 2+ ionophore-induced stimulation of TXB2 production in mouse plasma Enhancement (ex vivo) 10 mg/kg p.o. [81]
Plasma levels of PGE2 in rats with carrageenan-inflamed paw tissue Decrease 10–40 mg/kg p.o. [82]
Basal PGE production by mouse peritoneal macrophages Inhibition (ex vivo) 50 mg/kg p.o. [83]
IFN-γ, interferon-γ; LPS, lipopolysaccharide; LTB4, leukotriene B4; PGE, prostaglandin; s.c., subcutaneous; TNF, tumour necrosis factor; TXB2, thromboxane B2. *
Bell-shaped dose-response curve: CBD was more effective against clinical signs of arthritis (1) at 5 mg/kg/day i.p. than at 10 or 20 mg/kg/day i.p. and (2) at
25 mg/kg/day p.o. than at 50 mg/kg/day p.o. Optimal doses for reducing histologically assessed joint damage in arthritic mice were 5 mg/kg/day i.p. and 25 mg/kg/day
p.o.
†
Dose of CBD administered to the arthritic mice before they were killed.