ISOLATION AND CHARACTERIZATION OF ENTOMOPATHOGENIC ...

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LIU, Y.Z., MILNER, R.J., MCRAE, C.F. AND LUTTON, G.G., 1993, The use of dodire in selective media for isolation of Metarhizium spp. from soil. Journal of Invertebrate Entomology, 62: 248-251. LOPEZ, C.V., FERNANDEZ, I. AND FERRANDIS, B., 1995, Effect of heavy metals on growth isolation and proteolytic activity of the nematophagous fungus Verticillium suchlasporium. African Entomology, 13: 93-96. LORIA, R., GALAINI, S. AND ROBERTS, D.W., 1983, Survival of inoculum of the entompathogenic fungus Beauveria bassiana as influence by fungicides. Environmental Entomology, 12: 1724-1726. MADDOX, J.V., 1994, Insect pathogens as biological control agents. In Introduction to Insect Pest Management (Eds. Metcalf, R.L., Luckmann, W.H.). John Wiley and Sons Inc., Publication, New York, pp.199-244. MARGARET, S., BRUCE, L.P., DALE, R.B., 1991, Verticillium lecanii, isolated from larvae of pear Thrips, Taediothrips inconsequens, in Vermont. Journal of Invertebrate Pathology, 58: 157-163. MASUDA, T. AND KIKUCHI, O., 1992, Pathogenicity of Verticillium lecanii isolates to whitefly and aphids. Japanese Journal of Applied Entomology and Zoology, 36: 239-245. MAURER, P., COUTEAUDIER, Y., GIRARD, P.A. AND RIBA, G., 1997, Genetic diversity of Beauveria bassiana and relatedness to hose insect range. Mycological Research, 101: 139-164. McCAMMON, S.A. AND RATH, A.C., 1994, Separation of Metarhizium anisopliae strains by temperature dependent germination rates. Mycological Research, 98: 1253-1257. McCOY, C.W., SAMSON, R.A. AND BOUCIAS, D.G., 1988, Entomogenous fungi, In: Handbook of Natural Pesticides, Vol.5, Microbial Insecticides, Part A, Entomogenous Protozoa and Fungi, ed., C.M. Iqnoffo, CRC, Press, BocaRAton, Florida, USA, pp.151-236. McCOY, E.E. AND CARVER, C.W., 1941, A method for obtaining spores of the fungus Beauveria bassiava (Bals.) vill. In quantity, J.N.Y. Entomol. Soc. 49: 205. MEADE, D.L. AND BRUCE, D.N., 1991, The use of Verticillium lecanii against submarginal instars of Bemisia tabaci. Journal of Invertebrate Pathology, 57: 296-298. MILLER, R.W. AND BARRAN, L.R., 1977, The effect of ionic surface-active agents on macronodial plasma membrane of Fusarium sulphureum. Canadian Journal of Microbiology, 23: 1373-1383. MILNER, R., WRIGHT, T., WELTON, M., BUDAU, C., TYRRELL, B., 1992, Identification and partial purification of a cell lytic factor from entomophasa. Annual Meeting of Society for Invertebrate Pathology, 254: 230-260. MILNER, R.J. AND LUTTON, G.G., 1986, Dependence of Verticillium lecanii on high humidifies for infection and sporulation using Myzus persicae as host. Environmental Entomology, 15: 380-382. MILNER, R.J., 1992, Selection and characterization of staring of Metarhizium anisophiae for control of soil insects in Australia. In: Biological Control of Locasts and Grasshoppers (C.J. Lomer and C. Prior, Eds.), CAB International, London, pp.200-207 MILNER, R.J., SAMSON, P.R. AND BULLARD, G.K., 2002, FI-1045 : A profile of a commercially useful isolate of Metarhizium anisopliae var. anisopliae. Biocontrol Science and Technology, 12: 43-58. MIRANPURI, G.S. AND KHACHATOURIANS, G.G., 1995, Application of Beauveria bassiana and Verticillium lecanii against Saskatoon berry leaf aphid Acyrthosiphon macrosiphum. Journal of Insect Science, 8: 93-95. MOORE, D., DOURO-KPINDOU, O.K., JENKINS, N.E. AND LOMER, C.J., 1996, Effect of moisture content and temperature on storage of Metarhizium flavoviridae conidia. Biocontrol Science and Technology, 6: 51-61. MOORE, D., HIGGINS, P.M. AND LOMER, C.J., 1996, The effects of simulated and nautral sunlight on the viability of conidia of Metarhizium flavoviride Gams and Rozsypal and interactions with termperature. Biocontrol Science Technology, 7: 87-94. MORLEY, D.J, MOORE, D., PRIOR, C., 1995, Screening of Metarhizium and Beauveria spp. conidia with exposure to simulated sunlight and a range of temperatures. Mycological Research, 100: 31–38.
MULLER, E.J., 2000, Control of the brown locust, Locustana pardalina (Walker) using a mycoinsecticide addressing the issues of speed of kill, dose rate mortality and reduction in feeding. African Entomology, 8: 217-221. NADEAU, M.P. AND BOISVERT, J.L., 1994, Larvicidal activity of the entomopathogenic fungus Tolypocladium cylindrosporum (Deuteromycotina: Hyphomycetes) on the mosquito Aedes triseriatus and the black fly Simulium vittatum (Diptera: Simuliidae). Journal of the American Mosquito Control Association, 10: 487–491. NADEAU, M.P., DUNPHY, G.B. AND BOISVERT, J.L., 1996, Development of Erynia conica (Zygomycetes : Entomophthorales) on the cuticle of the adult black flies Simulium rostratum and Simulium decorum (Diptera : Simuliidae). Journal of Invertebrate Pathology, 68: 50-58. NEI, M. AND LI, W.H., 1979, Mathematical model for studying genetic variation interms of restriction endonucleases. Proceedings of National Academic Science, USA, 70: 5269-5273. NENI, Y.L. AND THAPLIYAL, P.N., 1997, Fungicides in Plant Disease Control. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, p.531. NEWTON, B.A., 1956, The properties and mode of action of the polymyxins. Bacteriological Review, 20: 14-27. NIER, T. RIVER, F. AND BERMUDEZ, J.C., 1993, First report for Mexico on the isolation of Verticillium lecanii from whitefly and in vitro pathogenicity tests on this insect. Revista Mexicana De Micologia. 7: 149-156. PARIS, J. AND FERRON, G., 1971, Virulence of some B. brongniartii mutants. Annual Review of Entomology, 16: 74-77. PATEL, K.C. AND YADAV, D.N., 1990, Laboratory mass production studies with Metarhizium anisopliae. Annals of biology (Ludhiana), 6: 135-140. PATIL, R.K., 2000, Ecofriendly approaches for the management of Spodoptera litura (F.) in groundnut. Ph.D. Thesis, University of Agricultural Sciences, Dharwad. PEKRUL, S. AND GRULA, E.A., 1979, Mode of infection of the corn earworm (Heliothis zea) by Beauveria bassiana as revealed by scanning electron microscopy. Journal of Invertebrates Pathology, 34: 228-247. PHADKE, C.H., RAO, V.G. AND PAWAR, S.K., 1978, Natural outbreak of the mycordine fungus, Nomurea rileyi (Farlow) Samson on leaf cating caterpillar, Sopdoptera litura Fab. in Maharashtra. Current Science, 47: 476-478. PREEZ, J.C., DU, JONG, F. DE., BOTES, P.J. AND LATEGON, T.M., 1985, Fermentation alcohol from grain sorghum starch. Biomass, 8: 101-117. PRIOR, C., JOLLANDS, P. AND PATOUREL, G., 1988, Infectivity of oil and water formulations of Beauveria bassiana to the cocoa weevil pest Pantorhytes plutus. Journal of Invertebrate Pathology, 52: 66-72. PRISTAVKO, W.P. AND GORAL, V.M., 1967, The mass production of Meauveria bassiana, In: Insect Pathology and Microbial Control, ed. P.A. Van dev. Lann. North, Holland Publishing Co. Amsterdam, pp.118-119. PUTTLER, B., IGNOFFO, C.M. AND HOSTETTER, D.L., 1976, Relative susceptibility of nine caterpiller species to the fungus Nomuraea rileyi. Journal of Invertebrate Pathology, 27: 269-270. PUZARI, K.C., SARMAH, D.K. AND HAZARIKA, L.K., 1997, Medium for mass production of Beauveria bassiana (Balsamo) Vuillemin. Journal of Biological Control, 11: 97- 110. QUINDEN, R.J., 1984, The use of Verticillium lecanii an entomopathogenic fungus to control of glasshouse whitefly. In Proceedings of a Conference at Brighton, England, 22- 25: 9-12. QUINTELA, E.D. AND McCOY, C.W., 1997, Pathogenicity enhancement of Metarhizium anisopliae and Beauveria bassiana instars of Diaprepes abbreviatus (Coleoptera:Curculionidae) with sublethal doses of imidacloprid. Environmental Entomology, 26: 11173-1182. RAMOSKA, W.A., 1982, An examination of the long term epizootic potential of various artificially introduced mosquito larval pathogens. Mosquito News, 42: 603-607. RAMOSKA, W.A., 1984, The influence of relative humidity on Beauvuria bassiana infectivity and replication in the Chich bug, Blissus leucopterus. Journal of Invertebrate Pathology, 43: 389-394.
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ISOLATION AND CHARACTERIZATION OF E
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Chapter No. I INTRODUCTION C O N T
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Contd….. Table No. Title 14. Spor
Page 7 and 8:
Contd….. Table No. Title 39. Per
Page 9 and 10:
Plate No. LIST OF PLATES Title 1. M
Page 11 and 12:
I.INTRODUCTION The increased use of
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II. REVIEW OF LITERATURE The variou
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The entomopathogenic fungi so far i
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Table 1. Contd….. Sl. No. Insect
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Milner et al. (2002) studied the ef
Page 21 and 22:
25°C for sporulation and biomass p
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Pathogencity of various strains/iso
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Gopalkrishnan and Mohan (2000) test
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necessary serial dilutions under ph
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Sl. No. Table 2. Details of differe
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3.9 EVALUATION OF DIFFERENT FORMULA
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Fig. 1. Mycopathogens of insect pes
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Table 5. Contd….. Month/s visited
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Table 5. Contd….. Month/s visited
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Table 5. Contd….. Mycosis noticed
Page 41 and 42:
Totally eight isolates of N. rileyi
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Metarhizium anisopliae Vertucillium
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Ma 1 (Dharwad) Ma2 (Dharwad) Ma 3 (
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Table 8. Mortality caused by the is
Page 49 and 50:
Helicoverpa armigera Dimond Black M
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Plate 5. The moratality of Hlelicov
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Table 11. Total biomass production
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Table 12. Total biomass production
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Table 14. Sporulation of Metarhiziu
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Mean spore yield x 10 8 /g 25 20 15
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Among the non-grain substrates, the
Page 63 and 64:
Mean spore yield x 10 4 /g 35 30 25
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Sl. No. Table 19. Effect of fungici
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Per cent inhibition 100 90 80 70 60
Page 69 and 70: Sl. No. Table 21. Effect of insecti
Page 71 and 72: Out of nine insecticides, endosulfa
Page 73 and 74: Sl. No. Table 23. Effect of weedici
Page 75 and 76: Days after storage Table 25. Persis
Page 77 and 78: Table 26. Survival of Metarhizium a
Page 79 and 80: Table 28. Survival of Metarhizium a
Page 81 and 82: Sl. No. Table 29. Survival of Metar
Page 83 and 84: Sl. No. Table 30. Survival of Metar
Page 85 and 86: Per cent germination 90 80 70 60 50
Page 87 and 88: Sl. No. Table 33. Survival of Verti
Page 93 and 94: 4.9 PATHOGENICITY OF Verticillium l
Page 95 and 96: Sl. No. Table 39. Per cent mortalit
Page 97 and 98: Culture grown on rice grains Harves
Page 99 and 100: Tr. No. Table 41. Per cent reductio
Page 101 and 102: Tr. No. Table 43. Per cent reductio
Page 103 and 104: The fungal spray at higher dosage (
Page 105 and 106: Table 46. Effect of wild types and
Page 107 and 108: The hyphal and conidial characters
Page 109 and 110: In an earlier experiment, bagasse w
Page 111 and 112: M. anisopliae exhibited rapid reduc
Page 113 and 114: higher mortality of aphids. The com
Page 115 and 116: 14. Oil formulations proved better
Page 117 and 118: CHARNLEY, A.K. AND ST. LEGER, R.J.,
Page 119: IGNOFFO, C.M., MARSTON, N.L., HOSTE
Page 123 and 124: ST LEGER, R.J., BUTT, T.M., STAPLES
Page 125 and 126: APPENDIX I Mean monthly weather dat
Page 127 and 128: Maharashtra Association for the Cul
Page 129: ISOLATION AND CHARACTERIZATION OF E
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