ISOLATION AND CHARACTERIZATION OF ENTOMOPATHOGENIC ...

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Identification Repost of the fungal cultures supplied by Dr. R.K. Patil, Dept. of Agril. Entomology, UAS, Dharwad-580 005 dt. 15.2.01
ISOLATION AND CHARACTERIZATION OF ENTOMOPATHOGENIC FUNGI AND THEIR EFFECTIVENESS BHARATHI H. TALWAR 2005 Dr. J. H. KULKARNI MAJOR ADVISOR ABSTRACT An attempt was made to isolate and characterize native entomopathogenic fungi. Nine insect mycopathogens, belonging to seven genera were found naturally occurring in the nine districts of Northern Karnataka. Morphological variation among the field collected isolates of M. anisopliae (Ma2) and V. lecanii (Vl1) for various parameters like, hyphal and conidial characters, sporulation and spore yield were recorded. Pathogenicity of V. lecanii to aphids, whitefly and mites and that of M. anisopliae to American bollworms and rhinocerous beetle was proved in laboratory. Among different carbon sources tested, starch proved superior for both the mycopathogens NH4 (SO4) and KNO3 were superior as nitrogen source. Bajra and rice grains served as most productive media for conidial growth of Ma2 and Vl1 with an yield of 22.77x10 8 and 24.59x10 8 conidia per g of media, respectively. Among pesticides tested, fungicides showed maximum inhibition followed by insecticides and weedicides. The persistence of mycopathogens was higher in soil (upto 16 months) than in phylloplane (upto 3 months). In general, all the carrier material had more conidial viability when stored in refrigerated and deep freezer condition and a reduction of more than 10 per cent and 8 per cent in refrigerated and deep fereezer was observed after 150 DAS respectively. Oil formulations proved better than wettable powder. Among the different concentrations of V. lecanii evaluated in laboratory against B. brassicae and Aleurodicus disperses, higher dosage of V. lecanii (1.2x10 9 conidia/ha) was found effective with highest mortality of 92.30 per cent and 80.93 per cent on the 10 th day after spraying respectively. A comparative account of efficacy of different isolates of V. lecanii under field conditions were evaluated against B. brassicae and A. crassivora. At the highest dosage of all isolates (2x10 12 conidia/ha), Vl1 proved most potent against B. brassicae (61.16%mortality), whereas Vl3 showed better control against A. crassivora (66.50%).
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ISOLATION AND CHARACTERIZATION OF E
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Chapter No. I INTRODUCTION C O N T
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Contd….. Table No. Title 14. Spor
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Contd….. Table No. Title 39. Per
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Plate No. LIST OF PLATES Title 1. M
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I.INTRODUCTION The increased use of
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II. REVIEW OF LITERATURE The variou
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The entomopathogenic fungi so far i
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Table 1. Contd….. Sl. No. Insect
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Milner et al. (2002) studied the ef
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25°C for sporulation and biomass p
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Pathogencity of various strains/iso
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Gopalkrishnan and Mohan (2000) test
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necessary serial dilutions under ph
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Sl. No. Table 2. Details of differe
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3.9 EVALUATION OF DIFFERENT FORMULA
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Fig. 1. Mycopathogens of insect pes
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Table 5. Contd….. Month/s visited
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Table 5. Contd….. Month/s visited
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Table 5. Contd….. Mycosis noticed
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Totally eight isolates of N. rileyi
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Metarhizium anisopliae Vertucillium
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Ma 1 (Dharwad) Ma2 (Dharwad) Ma 3 (
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Table 8. Mortality caused by the is
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Helicoverpa armigera Dimond Black M
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Plate 5. The moratality of Hlelicov
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Table 11. Total biomass production
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Table 12. Total biomass production
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Table 14. Sporulation of Metarhiziu
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Mean spore yield x 10 8 /g 25 20 15
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Among the non-grain substrates, the
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Mean spore yield x 10 4 /g 35 30 25
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Sl. No. Table 19. Effect of fungici
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Per cent inhibition 100 90 80 70 60
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Sl. No. Table 21. Effect of insecti
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Out of nine insecticides, endosulfa
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Sl. No. Table 23. Effect of weedici
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Days after storage Table 25. Persis
Page 77 and 78: Table 26. Survival of Metarhizium a
Page 79 and 80: Table 28. Survival of Metarhizium a
Page 81 and 82: Sl. No. Table 29. Survival of Metar
Page 83 and 84: Sl. No. Table 30. Survival of Metar
Page 85 and 86: Per cent germination 90 80 70 60 50
Page 87 and 88: Sl. No. Table 33. Survival of Verti
Page 93 and 94: 4.9 PATHOGENICITY OF Verticillium l
Page 95 and 96: Sl. No. Table 39. Per cent mortalit
Page 97 and 98: Culture grown on rice grains Harves
Page 99 and 100: Tr. No. Table 41. Per cent reductio
Page 101 and 102: Tr. No. Table 43. Per cent reductio
Page 103 and 104: The fungal spray at higher dosage (
Page 105 and 106: Table 46. Effect of wild types and
Page 107 and 108: The hyphal and conidial characters
Page 109 and 110: In an earlier experiment, bagasse w
Page 111 and 112: M. anisopliae exhibited rapid reduc
Page 113 and 114: higher mortality of aphids. The com
Page 115 and 116: 14. Oil formulations proved better
Page 117 and 118: CHARNLEY, A.K. AND ST. LEGER, R.J.,
Page 119 and 120: IGNOFFO, C.M., MARSTON, N.L., HOSTE
Page 121 and 122: MULLER, E.J., 2000, Control of the
Page 123 and 124: ST LEGER, R.J., BUTT, T.M., STAPLES
Page 125 and 126: APPENDIX I Mean monthly weather dat
Page 127: Maharashtra Association for the Cul
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