a study of the quality of a local herbal tea and volatiles of parinari ...

Distillation was carried out for 4 hrs and 33 minutes at reduced temperature because of
forming. The hydrosol (600 ml) was placed in a separatory flask, dichloromethane
(30 ml) and a saturated solution of NaCl (30 ml) were added. The mixture was swirled to
allow mixing and extraction of the compounds. The organic layer was separated and
collected in a separate container. Extraction with dichloromethane (30 mls) was done
twice as above. The three portions of organic extracts were collected, mixed and dried
with anhydrous Na2SO4. Filtration with glass wool was done to remove particles of
Na2SO4. The extract was concentrated on a vigreux column (30 cm) to approximately 2
ml. Further concentration was done by blowing a stream of nitrogen over the sample to
about 0.1 ml. The extract was stored at 5 0 C before GC-MS analysis (Saim et al., 2008).
3.7.3 Cold finger molecular distillation of F. ancylantha tea leaves
Dried F. ancylantha tea leaves (50 g), distilled water (200 ml) and NaCl (30 g) were
placed in a flat bottomed flask (1 L) which was then closed with a cold finger. The cold
finger was connected to a Knf Neuberger laboport vacuum pump (1.0 bars). Vacuum
pump was connected and the cold finger was filled with liquid nitrogen. The experiment
was run for 3 hours with the distillate condensing on the cold finger trap. The distillate
was extracted with 5 ml of dichloromethane. The aqueous layer was separated from the
organic layer and the organic layer was dried with Na2SO4. Concentration to about 0.1 ml
was done by blowing a gentle stream of nitrogen over the extract. The concentrate was
stored at 5 0 C before GC-MS analysis (Gazim et al., 2008).
45