a study of the quality of a local herbal tea and volatiles of parinari ...

done to remove particles of Na2SO4. The extract was concentrated on a vigreux column
(30 cm) to approximately 2 ml. Further concentration was done by blowing a stream of
nitrogen over the sample to about 0.1 ml. The extract was stored at 5 0 C (Saim et al.,
2008).
3.8.3 Hydrodistillation and solvent extraction from hydrosol of fresh
cassava leaves
Fresh cassava leaves (100 g) were placed in the still (1 L), double distilled water (500 ml)
with antibumping granules were added to the still. Distillation was carried out for
2 hrs and 51 minutes using a clevenger apparatus. Double distilled water (200 ml) was
being added at hourly intervals. The hydrosol (600 mls) was placed in a separatory flask;
dichloromethane (30 ml) and a saturated solution of NaCl (30 ml) were added. The
mixture was swirled to allow mixing and extraction of the compounds. The organic layer
was separated and collected in a separate container. Extraction with dichloromethane (30
ml) was done twice as above. The three portions of organic extracts were collected,
mixed and dried with anhydrous Na2SO4. Filtration with glass wool was done to remove
particles of Na2SO4. The extract was concentrated on a vigreux column (30 cm) to
approximately 2 ml. Further concentration was done by blowing a stream of nitrogen
over the sample to about 0.1 ml. The extract was stored at 5 ⁰C (Saim et al., 2008).
3.8.4 Hydrodistillation and solvent extraction from hydrosol of fresh
cocoyam leaves
Fresh cocoyam leaves (100 g) were placed in the still (1 L), double distilled water (500
ml) with antibumping granules were added to the still. Distillation was carried out for 3
hours and 57 minutes. Double distilled water (200 ml) was being added at hourly
intervals. The hydrosol (610 ml) was placed in a separatory flask, dichloromethane
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