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a study of the quality of a local herbal tea and volatiles of parinari ...

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1 0 C/min. Helium was used as a carrier gas at a constant flow <strong>of</strong> 1.2 ml/min. Spectra were<br />

obtained on electron impact <strong>of</strong> 70 eV, scanning from 35 to 550 m/z. n-Alkanes (C6-C24)<br />

were used as reference points in <strong>the</strong> calculation <strong>of</strong> retention indices (RI) Library searches<br />

were carried out using NIST, Mass Finder, Flavour <strong>and</strong> <strong>the</strong> Baser library <strong>of</strong> essential oil<br />

constituents by comparing mass spectra <strong>and</strong> retention indices (Kamatou et al., 2010).<br />

The retention index RI for an individual compound RIC was calculated using <strong>the</strong> formula:<br />

RIc = 100Z +100((log t`RC – log t`RZ)/(log t`R(Z+1)- log t`RZ))<br />

where t`RC, tRZ <strong>and</strong> t`R(Z+1) are <strong>the</strong> corrected retention times for <strong>the</strong> compound <strong>and</strong> <strong>the</strong><br />

alkanes eluting before <strong>and</strong> after <strong>the</strong> compound, respectively (Braithwaite <strong>and</strong><br />

Smith, 1996).<br />

3.11 Data analysis<br />

One way analysis <strong>of</strong> variance (ANOVA) was performed using Genstat 13 th edition to<br />

establish <strong>the</strong> differences in <strong>the</strong> mean levels <strong>of</strong> <strong>quality</strong> parameters studied between<br />

F. ancylantha <strong>tea</strong> <strong>and</strong> <strong>the</strong> control <strong>tea</strong>s. The significant differences among <strong>the</strong> means were<br />

tested at 95% confidence interval.<br />

54

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