a study of the quality of a local herbal tea and volatiles of parinari ...

(30 ml) and a saturated solution of NaCl (30 ml) were added. The mixture was swirled to
allow mixing and extraction of the compounds. The organic layer was separated and
collected in a separate container. Extraction with dichloromethane (30 ml) was done
twice as above. The three portions of organic extracts were collected, mixed and dried
with anhydrous Na2SO4. Filtration with glass wool was done to remove particles of
Na2SO4. The extract was concentrated on a vigreux column (30 cm) to approximately 2
ml. Further concentration was done by blowing a stream of nitrogen over the sample to
about 0.1 ml. The extract was stored at 5 ⁰C (Saim et al., 2008).
3.8.5 Hydrodistillation and solvent extraction from hydrosol of dried
cocoyam leaves
Dried cocoyam leaves (50 g) were placed in the still (1 L), double distilled water (500
ml) with antibumping granules were added to the still. Distillation was carried out for 3
hours and 3 minutes. The hydrosol (200 mls) was placed in a separatory flask,
dichloromethane (30 ml) and a saturated solution of NaCl (30 ml) were added. The
mixture was swirled to allow mixing and extraction of the compounds. The organic layer
was separated and collected in a separate container. Extraction with dichloromethane (30
ml) was done twice as above. The three portions of organic extracts were collected,
mixed and dried with anhydrous Na2SO4. Filtration with glass wool was done to remove
particles of Na2SO4. The extract was concentrated on a vigreux column (30 cm) to
approximately 2 ml. Further concentration was done by blowing a stream of nitrogen
over the sample to about 0.1 ml. The extract was stored at 5 0 C (Saim et al., 2008).
48