- Page 1 and 2: THE UNIVERSITY OF MANITOBA STUDIES
- Page 3 and 4: To My parents and wife: Mn & Mrs. K
- Page 5 and 6: CONTTNT S I. REVIEW 0N PLACENTAL PR
- Page 7 and 8: V. RESULTS E. STUDIES ON THT BIOSYN
- Page 9 and 10: Peptide Hormones: ACTH CG FSH GH LH
- Page 11: LIST OF FIGURES FIGURE PAGE I Linea
- Page 15 and 16: Effect of PGF,. and lactogenic horm
- Page 17 and 18: ABSTRACT STUDIES 0N 0VINE PLACENTAL
- Page 19 and 20: eceptor-b i nd i ng studies show th
- Page 21 and 22: -2- years have seen an increasing i
- Page 23 and 24: -4- secretion of a peptide hor:none
- Page 25 and 26: NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
- Page 27 and 28: 6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
- Page 29 and 30: -8- 197J-; Rayford et al | !9721 .
- Page 31 and 32: -10- sialic acid díd not alter the
- Page 33 and 34: -L2- and Biasucci (1969) denonstrat
- Page 35 and 36: _14_ addition of CAMP (Handwerger e
- Page 37 and 38: -16- conponents I the.faster haying
- Page 39 and 40: _18_ by hcc, as evidenced by increa
- Page 41 and 42: _20- (Mason et a1 , L973i Menon ¡
- Page 43 and 44: 21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
- Page 45 and 46: -23- growth-promoting ef,fect, wher
- Page 47 and 48: -25- diffusion teÊt. Josi¡novich
- Page 49 and 50: -27- v¡hereas in the tissue extrac
- Page 51 and 52: -29- found products of molecular we
- Page 53 and 54: -3r- vÍii) S.ecreLion and metabol
- Page 55 and 56: - 33 - metabolic factors may influe
- Page 57 and 58: -35- Although hpI, si¡nuLates the
- Page 59 and 60: -37- C. CIIHER ITUMAN PLACENTAL PEP
- Page 61 and 62: _39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
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109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
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EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
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SECTION V : RESULTS (r) LACTOGEN DU
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127 Fractioñâtión of oPL. from c
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129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
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TABLE I TABLE OF PURIFICATION OF OV
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137 Figure lì. A, Poìyacrylamide
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Leaf blank to correct nr.rmbering
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14r Anal.vtical Sodi um Dodecyl Sql
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o 9 x F- -)- 9 rU I æ -t f () LT'
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¿ o s : t45 Figure 15b. Displaceme
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HoR MoNE coNc€NrRArto ln ß/mt )
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Tissue used for binding ôtudies Li
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Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
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¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
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P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
- Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
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F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
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! o 't6 l HORMO¡¡ E COr{CENr FAIt
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163 Figure 24 shows the sensltivity
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165 D. STUDIES ON THE SECRETION OF
- Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
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lJhen blood samples were taken four
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Sheep no. l{6 25í 960 464 5?0 680
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't73 fetal cotylêdons at various d
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TABLE VI OPI CONCENTRATTON IN FETAL
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l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
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dUrlng PrêOnancy in thè Ewes tBr
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! ñ' l83 NoRMON! C oNCENTßArlO þ
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l ol
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I I I "+ _t rl \l :- 'i rl -l l87 a
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l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
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- 193 3H -couNdro _________SPECtFtC
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TABLE IX /- BIOASSAY OF qROIItIfH p
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z o 197 Figure 40, Radioreceptor as
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5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
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SFCT.IoN II : pl.scussr0N 4d ã 1È
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Ch aracteri zati on 203 Analytical
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205 size, corresponding to the mole
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207 the specific binding of oPL to
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209 than with prolactin-like effect
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2t1 the jncrease in oPRL binding si
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213 The resuìts obtained indicate
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215 on the role af PL in the fetus.
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Surgi ca1 method- 217 Our studies o
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219 5.Relationships amonq opRL. oGH
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221 The nature of thelarge nolecula
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223 data argue for a close interact
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]. TABLE OF CHEMICAL PROPERTIES GUI
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cor,t coAT (bPL) (cpl) 3. TABLE OF
- Page 251 and 252:
À) E 229 Figure 43. The secretion
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231 H. POSSIBLE ROLT OF OVINE PLACE
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233 Our studies on the blnding of o
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235 through its binding to cotpus l
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237 Furthermore, oPL neasurement in
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239 circulationand alìantoic f'lul
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SECTICN IX PUBLICATIONS A) Manuscr
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SECTION X: BIBLIOGRAPHY Abramovich,
- Page 267 and 268:
Beas,F., Saìinas, 4., GonzalezrF.,
- Page 269 and 270:
247 Brody,S,, and Carlstrom,G.,J.Cl
- Page 271 and 272:
249 Crosignanì ,P.G. , Nencioni ,
- Page 273 and 274:
25t Friesen,H.G., Endocrinoìogy 76
- Page 275 and 276:
253 Grinwich,D.L., M. Hichens, and
- Page 277 and 278:
25s Hum, V.G., Knipfel , J.G., and
- Page 279 and 280:
?57 Knopp,R,H,, H,J. Ruder,E. Herra
- Page 281 and 282:
259 Manku,M.S., and D.F. Horrobin,
- Page 283 and 284:
261 , :iir .,.-;;r-,:, Morgan, F.J.
- Page 285 and 286:
263 Pierce,G.B., Midþley,A.R. and
- Page 287 and 288:
?65 Shepard, T.H.., Gen. Comp. Endo
- Page 289 and 290:
267 Tsuruhara,T., Dufau,M.L., Hickm