THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

_46_
Tullner, 1968) were thè. first to make semi^quantitative
estimates of the concentration of cG in the seru¡n and. urine
of the intact and ovariectomized rhesus monkey in early
pregnancy by mouse uterus bioassay using hCG as standard.
When two hundred and fifty-seven serum samples fron 136
pregnant monkeys were collected from the 9th to the 4gth
day after nating and assayed for CG activity, CG was first
found in the serum J-5 days after mating, and was present,
in all sera until the 28th day after mating. With one
except.ion, mCG lvas not found in sera colLected 35 days after
mating. Maximum amounts of serum gonadotropin, equivalent
to 3.4 I.U. hcc per ml , were found between the 21st and 30th
days after mat.ing. Vùhen urinary mCG was determined on
24-hour colLections from five pregnant monkeys bet$reen the
21st and the 41st day after mating, the great.est amounts
of mCG vrere excreted also betr^reen 21st and 26th days of
pregnancy. Simílar results $rere aLso obtained by Arslan
et al (1967).
With the discovery that the chorionic aonadotropins
from monkey wiLl react in immunoassay systems which were
designed to measure gonadotropins of human origin (Tullner
et al, l-969; Nixon et aL, 1971 ; V,fide et a1 , lg7l) r monkey
chorionic Aonadotropín in the serum, urine and placental
extracts has been quantitãted. By employing the radio-
immunoassay, Hodgen et aI (1974) measured mcc in the
serul and urine fro¡n I to 12 days after the conception.
When serun progesterone was measured simultaneously in these