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260 Meites,J., and C'lemens,J.A., Vit.and Hormones 30:ì66,ì972 Meites,J,, in Mjlk: the manrmary gland and Ìts secretion, Chap I, pp321, eds. S.K. Kon and A.T. Cowie, N.y. and London: Acad press, 1961 McGarry, E.E., and,J.C.Beck, in Wolstenholme, G.E.l,l., and J. Knight (eds), Lacotgenic Hormones, Churchi l I -Livingston,London,p36l,l 972 Midgely,A.R., Jr., and Jaffe,R.B., J.C1ìn. Endocr. Metab. ZB:1712,.l968 Miyai,K., Tanizawa,0., Yamamoto,T., Azukizawa,M., Kawi,y., Noguchì,M., Ishibashi,K., and Kumahara,y., J. Clin Endocr. Metab. 42:254,.l976 Meyer, R.K., Wolf,R.C., and Arslan,M., proc. ',nd. INt. Congr. primate, Vo] .2, Karger. Base'l and N.y.,1969 Mochizukì,M., Morikawa,H., Tanaka,y., and Tojo,S., Endocr. proc. INt. Congr.,4th,l972, Excerpta Med. Found. INt. Congr, Ser. no.273 Abstr. pl4,l9l3 Mochizuki,M., Morì kawa,H.,0hga,Y.,and Tojo,S., Endocr. Jpn,ZZ l23,19l S Moore,N.l'1., and L.E.A. Rowson,Nature ì84:1410,.I959 Moore, R.M., and L.E.A., Rowson, J. Reprod. Fert. ll:307,1966a Moore, R.M., and L.E.A. Rowson,J. Endocr. 34:233,1966b Moore,R.M., and L.E.A.Rowson, J. Reprod. Fert. l2:539,.l966b l'loore, R.M., and L.E.A. Rowson, J. tndocr. 34:497,.l966d Morgan, F.J., Karmerman,S., and R.E. Canfieìd, in Gonadotroplns, ed. by B.B. Saxena, C.6. Be'l ing, HM Ganday, N. Y.,l'li ley-Interscience,p2ll,1g7Z Morgan,F.J., and ç¿¡¡ls1¿, Endocr. 88:.l045,t971 llorgan,F.J., Bìrken,S. , and Canfieìd,R.E., J. Biol Chem. 250:5247,.|975 MoÉan,F.J.,Canfiìed,R. E., Vaitukait.ls,J.L., and Ross,G.T., Endocr. 94:1601 ,1974
261 , :iir .,.-;;r-,:, Morgan, F.J., Birken,S., and Canfie'ld,R.E., J.Biol. Chem. 2S0tSZ4l ,1975 Mori, K.F., Endocr. 86:97,1970 Morris,H.H.B., Vinlk,A.I., and Mulhival ,M., Am.J. Obstet. Gynecol .lì9: 224 ,197 4 Moyle,l{.R., and Ramachandran,J., Endocrinology 93:127,1973 Munro,H.N,, and Steinert,p.m., Biochem., Ser. One. 7:359,1974 Iifurakawa,S., and R.S. Raben,tndocrinoìogy g3:645,ì96g Ívþant ,N.B.,in Thyroid Gland"(R. pitt-Rivers and l,i.R. lr.itter,eds),Vol . 4, p/. Acad Prees,N.Y.,l964 Nagasawa,H.,R. Yanl ,and K. yamanoudl , J. Endocr. 7ì:lì5,1976 Nandi,S.,Unlv. of Calif. Publicailons in Zoology6S:1,.l959 Nelson,l,l.0., Proc. Soc. Exp. Bio'l . Med. 33:222,.l935 Nelson,J.H., Hal1,J.t., Manual-Limson,G., Friedenberg,H., and 0rBrien,F.J., Am.J. 0bstet. Gynecol . 9B:B9S,l96Z Neil1,J.D., Handbook of pHysiology, Vol . IV. ed. E. Knobiì and l.l.H. Sawyer, Waberlly Press. Inc., Baltimore, pp 469,1974 Neil l,J.D., Johansson,E.D. 8., and Knobil,E.,Endocr.inology g4:45,.l 969 Nei1l,J.D., and Knobiì,8., Fed. Proc. ZB 772,'1969 Neri,P., TariirP., Arezzini,C., Cana'li, G., Cocola, F., and Tarli,p. Growth Hormormone , Proc. INt. Synp., 2nd., 197'l Excerpta Med. Found., INt. Congr. Ser. No. 244,pp 199,1972 Neri,P., Arezìnni,C. , Botti,R. , Cocola,F., and Tarll,p., Bioch.im. Biophys. Acta, 322:88,ì973 Newton,M.H. and N. Beck, J. tndocr. 'l :65,1939 Njall ,H.D. , Nature(London), New Biol . 230:90,197ì Nial l,H.D,, Hogan,M.L., Saùer.., R., Rosenblun,I.y., and Gresn¡¡qgd,p.[., Proc. Natl . Acad. Sci. USA 68:866,1971
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
Page 3 and 4:
To My parents and wife: Mn & Mrs. K
Page 5 and 6:
CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
Page 11 and 12:
LIST OF FIGURES FIGURE PAGE I Linea
Page 13 and 14:
22 Radioreceptor assay for oPRL us.
Page 15 and 16:
Effect of PGF,. and lactogenic horm
Page 17 and 18:
ABSTRACT STUDIES 0N 0VINE PLACENTAL
Page 19 and 20:
eceptor-b i nd i ng studies show th
Page 21 and 22:
-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
Page 25 and 26:
NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
Page 27 and 28:
6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
Page 29 and 30:
-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
Page 33 and 34:
-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
Page 43 and 44:
21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
Page 45 and 46:
-23- growth-promoting ef,fect, wher
Page 47 and 48:
-25- diffusion teÊt. Josi¡novich
Page 49 and 50:
-27- v¡hereas in the tissue extrac
Page 51 and 52:
-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
Page 59 and 60:
-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
Page 73 and 74:
B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
Page 77 and 78:
55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
Page 81 and 82:
(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
Page 87 and 88:
Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
Page 113 and 114:
9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
Page 119 and 120:
97 buffer, the eluates were pooled,
Page 121 and 122:
Char4ctêf ization Pfocedures l. Ch
Page 123 and 124:
t0t (c) À.nalvticaL .{rod.iu¡n do
Page 125 and 126:
103 B : MFTÍÍOD OF DETECTION AND
Page 127 and 128:
AFsa)¡- ProceiiureS 105 Specific b
Page 129 and 130:
107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
Page 135 and 136:
(4) Tissúe distributiori df radloi
Page 137 and 138:
l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
Page 141 and 142:
(2) Assav of ìuteo.trgpic effec.t
Page 143 and 144:
EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
Page 147 and 148:
SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150:
127 Fractioñâtión of oPL. from c
Page 151 and 152:
129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
Page 155 and 156:
E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158:
TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160:
137 Figure lì. A, Poìyacrylamide
Page 161 and 162:
Leaf blank to correct nr.rmbering
Page 163 and 164:
14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166:
o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168:
¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170:
HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172:
Tissue used for binding ôtudies Li
Page 173 and 174:
Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176:
¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178:
P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182:
F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184:
! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186:
163 Figure 24 shows the sensltivity
Page 187 and 188:
165 D. STUDIES ON THE SECRETION OF
Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
Page 191 and 192:
lJhen blood samples were taken four
Page 193 and 194:
Sheep no. l{6 25í 960 464 5?0 680
Page 195 and 196:
't73 fetal cotylêdons at various d
Page 197 and 198:
TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200:
l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
Page 203 and 204:
dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206:
! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208:
l ol
Page 209 and 210:
I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212:
l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
Page 215 and 216:
- 193 3H -couNdro _________SPECtFtC
Page 217 and 218:
TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220:
z o 197 Figure 40, Radioreceptor as
Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224:
SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226:
Ch aracteri zati on 203 Analytical
Page 227 and 228:
205 size, corresponding to the mole
Page 229 and 230:
207 the specific binding of oPL to
Page 231 and 232: 209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252: À) E 229 Figure 43. The secretion
Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256: 233 Our studies on the blnding of o
Page 257 and 258: 235 through its binding to cotpus l
Page 259 and 260: 237 Furthermore, oPL neasurement in
Page 261 and 262: 239 circulationand alìantoic f'lul
Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
Page 281: 259 Manku,M.S., and D.F. Horrobin,
Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm
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