THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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Figure 24 shows the sensltivity and specifÍclty of the
RIA for oPL. 0vine pituitary cH(ocH) and ovlne pRL(opRL) do not
cross-react (not shown). Simi.larly, several hormone preparations froûn
several specÍes either of pituitary or placental orlgln showed no
cross-reaction(not shown). The only sampìes which cross_reacted in
the assay were pregnant sheep placental extracts (after 20 days of
gestation), fetal serum (not shown), and pregnant sheep serum. These
resuìts indicate that opl is inmunologically different from sheep
pÌtuitary GH and pRL and also a variety of óther hormone preparation.s,
The ì ntraas say (three pregnant sheep plasma sampìes assayed l0 tÍmes in the
same assay) and lnterassay (sÍx pregnant sheep plasma assayed fn five
consecutive assays) coefficients of variation were ì0.51 and 8.21,
respect.ively.
The hormone preparations tested w¿¡shGH(NIH 20.t9G,2.2 IU/mg),
MGH(NIH M.945A,O.gIU/MS), bGH(NTH B-IOO3A, 2.OIU/Mg),OGH(NIH 0-7438,
1.2 IU/nsl, bpRL(NrH p-Br, tSIU/mg), opRL(NtH_p_S-10,26rUlng), hPL(tttH,
95% electrophoretically pure), hpRL, mpl, rpL, cpl, and bpL(all placental
ìactogens were purified în our laboratory¡ each have ì0_301 potency of the
oPRL standard when assayed in the RM-pRL).These hormoñes.were tested at
concentrations as high as l0 uglm] .
The p,lacental extracts tested were human, monkey, cow, horse,
dog, rabbit, rat, mice, hamster, and guinea plg. Sfnrllarly,
pituitary extracts of these species were also tested.