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256 Kanazawa, S., Nakamura,4., Saida,K., and Tojo,S., Acta Obstet. Gynecol Scand. 55:201 ,1976 Kap1an,S.L., and Grumbach,M.M., J. Clin. Endocr. 24:80,.l964 Kapìan,S.L. " and M.M.Grum bach, Science 147: 751 ,.|965 Kaplan, S.1., Gurpid,E., Sciarra,J..l., and Grumbach,M.14., J. Clin. tndocr. Metab.2B:.|450,ì969 Kaplan'S.1., and M.M. Grumbach, in Lactogenlc Hormones, Fetal Nutrition, and Lactation, Voì. 2, ed. Joslmovich,J.B., and M. Reynoìds, and E.Cobo, John üliley & Son, New york,USA,plB3,t974 Kaplan,s.L., Grumbach,M.M., and Aubert,M.L., Recent prog. Hormone. Res, 32: l6l ,.I976 Karsch, F.,.!., Cooke, 8., E'lliot,A.R., Foster, D.1., Jackson,G.L., and Nalbandov,A.V., Endocrinolo gy g9zZ7Z,197l Kasakura,K., J. Inrnunol. 107:.1296,1971 Kave, 14.D., and Jones,}'l.R., Am. J. Obstet. Gynecoì.,.l09:.l029,197.| Keì1er,P.J., Ruppen,M., Gerber,c., and schmld,J., J. Obstet. Gynaecor.Br. Conrnonw, 79:804 ,1972 Ke1ìy, P.4., H.A. Robertson and H.G. Friesen, Nature Z4B:435,ì974 Keìly,P.4., T. Tsushima, R,C. Shiu and H.G. Friesen, Endocrinology 99:765,1976 Kenimer, J.G., Hershman,J.M., and Hlggins,H,p., J. Cìln. Endocr. Metab. 40:482,'1975 Kido,I. Zentralbl . Gynaekol . 6l:ìS5l ,.l937 Kikutani,M., and Tokuyasu, K.,,J. Blochem. (Tokyo)57:59g,196s Klm,Y.J., and Felig.P..,J.ClJn. Endocr. Metab. 32:864,.|977 Kleinberg, D.L. and A.G. Frantz,J.C.lln. Invest. 50:1557,t97ì
?57 Knopp,R,H,, H,J. Ruder,E. Herra, and N. Frienkel ,Endocrinology 65:352,.l970 Koch, Y., Zor.U", Chobs.ieng,p., Lamprecht,S.A., pomerantz,S., and Lindner,H. J. Endocrìnology 6l :179,.1974 Kock,H,, von Kesseì, H., Stoìte,L., and von Leusdem,H., ,!. Clin. Endocr. Metab. 26 : I I 28 , l 966 Kohmoto,K., and H.A. Bern,H,A., J. Endocr. 4g:99,1970 Kohmoto,K., and H.A. Bern, H.A., J. tndocrinol . 49:29,.1970 Kohler, P.0., Ross,G.T. n and Odell,l,ü,D., J. C.lin.Invest.47:3g,.l96g KovacÍc,N., J. Endocr. 35: xxv. 1966 Kurosaka, M., Tohoku J. Exp. Med. Z4:t6i,.196.| Kosasa, T. S. ,Levesque ,L.A . , Taynor,M.L., and GoldstelnrD.p., Fertiì, Ster.il . ZS:Z11 ,1914 Kraìncanìc,M., Genbacev,0,, and Sulovic,V. , Jugosl . physioì. pharmacol . Acta II:99,1975 Laube, H,, Fussganger,R.D., and Schroder,K.E., Diabetes 21 :1072,1972 Lauritzen,C., and Lehman,l,l.D., J. Endocr. 39:.l73,1967 Lauritzen,C., Shackleton,C.H, 1., and Mitchell,F.L,, Acta Endocr. Copenhagen 61 :83,1969 Leader,D.P, Biochem.Soc. Trans.3:257,ì925 Leat,R.A.F.,Proc. Nutr. Soc. 30:236,t97.l Lee'c.Y., and Ryan,R.J. ,ln " Structu re-Acti vi ty Relationships of protein and Polypeptide Hormone,' (M. Margou I i s and F.C. Greenwood,eds), Int. Congr. Ser. No. 24.l ,part Z, p232. Exerpta Med. Found., Amsterdam,ì 972 Lee,C.Y., and Ryan,R.J.,Biochem. l2:4609,1973 Lee,C.Y., and Ryan, R.J., Endocrinology 95:.l69.l ,.l975
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
Page 3 and 4:
To My parents and wife: Mn & Mrs. K
Page 5 and 6:
CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
Page 11 and 12:
LIST OF FIGURES FIGURE PAGE I Linea
Page 13 and 14:
22 Radioreceptor assay for oPRL us.
Page 15 and 16:
Effect of PGF,. and lactogenic horm
Page 17 and 18:
ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
Page 25 and 26:
NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
Page 27 and 28:
6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
Page 29 and 30:
-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
Page 33 and 34:
-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
Page 47 and 48:
-25- diffusion teÊt. Josi¡novich
Page 49 and 50:
-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
Page 59 and 60:
-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
Page 143 and 144:
EXPERIMENT # TI (7 days after HCG)
Page 145 and 146:
123 to progesterone (l/300 djlution
Page 147 and 148:
SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150:
127 Fractioñâtión of oPL. from c
Page 151 and 152:
129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158:
TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160:
137 Figure lì. A, Poìyacrylamide
Page 161 and 162:
Leaf blank to correct nr.rmbering
Page 163 and 164:
14r Anal.vtical Sodi um Dodecyl Sql
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o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168:
¿ o s : t45 Figure 15b. Displaceme
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HoR MoNE coNc€NrRArto ln ß/mt )
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Tissue used for binding ôtudies Li
Page 173 and 174:
Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176:
¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178:
P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182:
F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184:
! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186:
163 Figure 24 shows the sensltivity
Page 187 and 188:
165 D. STUDIES ON THE SECRETION OF
Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
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lJhen blood samples were taken four
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Sheep no. l{6 25í 960 464 5?0 680
Page 195 and 196:
't73 fetal cotylêdons at various d
Page 197 and 198:
TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200:
l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
Page 203 and 204:
dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206:
! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208:
l ol
Page 209 and 210:
I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212:
l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
Page 215 and 216:
- 193 3H -couNdro _________SPECtFtC
Page 217 and 218:
TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220:
z o 197 Figure 40, Radioreceptor as
Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224:
SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226:
Ch aracteri zati on 203 Analytical
Page 227 and 228: 205 size, corresponding to the mole
Page 229 and 230: 207 the specific binding of oPL to
Page 231 and 232: 209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252: À) E 229 Figure 43. The secretion
Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256: 233 Our studies on the blnding of o
Page 257 and 258: 235 through its binding to cotpus l
Page 259 and 260: 237 Furthermore, oPL neasurement in
Page 261 and 262: 239 circulationand alìantoic f'lul
Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
Page 277: 25s Hum, V.G., Knipfel , J.G., and
Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm
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