THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

TABLE I
TABLE OF PURIFICATION OF OVINE PLACENTAL LACTOGEN
PURIFICATION FACTOR
I
j
*
PROCEDURE PROTEIN RRA-GH RIA-oPL REC0VERY IN Z OF
(s) (ms) (ms) pR0TEIN RRA:cH RrA-opL
Extraction 646 390 3.l2 .l00 l0O 100
Acidification 546 370 294 g5 95 94
(pH 6.s)
2
(¡
3
DEAE-Cellulose 150 ]53 120 23 39 38
Acidification 103 134 .l14
16 34 37
(pH 6.0)
34
CM-Cel l ulose 3,2 67 52 O.S 17 17
86
CM-Sephadex 0.65 32.2 27.1 0.10 8.3 8.7
( c-5b)
446
Sephadex G-100 0.103 23.8 2Z.Z 0.016 6.1 7.1
1685
DE-Sephadex 0.021 '15.6 '17.ì 0.0033 4.0 5.s
( A-25 )
Sephadex G-S0 O.OIS** ß.t 16.3 0.0023 3.8 5.2
* Protein measured by Lowry method
** Dry welght
N.B. This procedure was empioyed for the purificatìon of oPL uslng 3 separate batches of pìacentaS
yielding ' a total of 35 mg oPL. In general , the results were simllar to the data shown above.