THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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B.: DETECTION ôND CHARACIERIIATIgN 0F THF RECEIT0R .F9R 0VINE. PLACENTAL
LACTOGEN IT THE SHEEP
Tissue Survey
The results of the tissue survey are shown in Tab'le II.
Hìgh specific blnding of oPL to '100,000 x g pellet was found for the
liver, adipose tissue, ovary, corpus luteum, and uterus of the ewe,
and the fetal liver. In the remaining tissues, specific blnding lvas less
than 3%.
Effect of Prôtein Concentrat.ion on the SÞecific BÍnding of l25I-opl
to different Ti ssues
Figure ì7 shows that in .100,000 x g fractions from
several tissues specific binding of opL lncreased as the amount of
protein i ncreased.
Effect of Incubation Time and Temperature on the Binding of Ovine
Pìacental Lactogen to Different Tissues
Figure IBA shows the effect of lncubation time and temperature
on the specific binding of oPL to ì00,000 x g pellets obtained from
liver and adipose tissue of the non-pregnant ewe and from corpora
lutea of pregnant ewes, Specifïc binding was maxÍmal at 24 h for all
tissue binding sÌtes when the incubation was performed at 4 C. F.igure
1BB depicts similar results when lOO,000 x g pellets fron the ovary
and uterus of the non-pregnant ev,,e and the liver of a fetus obtalned