THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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be proportional to the amount of protein present. However, if
we assume that the intensity of the stained band is proportionaì
to the amount of protein present and is similar for al'l
proteìns than I could conc'lude that oPL constituted
mor€ than B0% of the protein in the preparation.
Analyticaì ge1 isoeìectric focusing-
In the present studies, we empl oyed cytochrome C
as an indicator and assumed that a steady state gradient was set
up for oPL when cytochrome C passed over the cathode. Whether
this was the tinre that a steady-state had been established
renains uncertain. A.gain, by emp'loying this sensitiv.e technique,
3 stained bands were separated by at least 0.3 cm. The presence
of GH-like activity wasdetectedin eìuants from seqments which
corresponded to the two stained bands. Some over1ap in activity
of the least stained band was also apparent.
Estimation of molecular ureioht by polyacrylamide gel (slab)
containinq sodìum dodecyl sulfate (SnS)-
Comparison of the mobilities of opl with other
known molecular weight proteins in po'lyacry'lam i de gels
containing SDS indicate that oPL, hPL, and opRL are of similar