254 Henzl ,M.R., and E.J. Serg, Contraception 1:315,1970 Herrera,E., R.H.Knopp, and N. Freinkel , J. Clin INvest. 48:2260,1969 Haour,F., Tell,G., and Sanchez,P., V. Int. Congr. Endocr., Hamburg,4b.777'lg Hisaw, F.1., Yaìe J. Bio'l . Med. l7:119,.l944 Hoìst,P.A. ¡ and Phemister, R.D., Biol .Reprod. 5:'194,197'l Hwang, P., H. Guyda, and H. Frleeen, J. Biol . Chem 247:1955,1972 Haustraete, F., Ver Eecke, T., I'lous,,l., Peeters,B., and Romboutesrlrl., Arch. Int. Physiol. Biochim. 83:973,1975 Hennen, C.P., Arch. INt. Physiolo. Biochim. 73:689,.l965 Hennen, G.P., Arch INt. Physiol . Biochìm. 74:303,.l966a Nennen,G.P., Arch. Int. Physio'l . Biochim. 74:70'l ,.l966b Hennen,G.P., J. Clin. Endocr. Metab. 27:610,.l967 Hennen, G.P., and Freychet,P., Isr. ,J. Med. Sci. l0:1332,1974 Hennen,G.P., and Pierce, J.G., Protein Polypeptìde Honnone , Proc. Int. Syltp., Excerpta l4ed, Found. , Int. Congr. Ser. No. ì6'l , Part I I, p51 I ,ì 969 Hershman, J.M. and Burrow,G.N., J. Clin. tndocr. Metab. 42:970,1976 Hershman,J.M., and Starnes,t'l. R., J. Cl in. Endocr. ìletab.32:52, 1971 Hershman,J.M., Higgins, H.P., and Starnes, l,|.R., Metab., Clìn. Exp. I 9 :735 ,'l 970 Hilgenfeldt, U., Merz, l,'1.E., and Brossmer,R., Hoppe-Seyler's Z. Physiol . Chem. 355:105.l ,1974 Hobson,B.M., and l.lide,L., J. Endocrinol . 60¡75,1974 Hobson,B.M., and Wide,L., J. Endocrinol .64:,l17,1974 Huìstart, C.E.,Torring,J.L., Koudstaal ,,J., Hardonk,Mrl..and Mo'lenaar,I. Gynecol . Invest. 4:24,1973 Hodgen,G.D.,l^l.W.Tullner,J.L.Vaitukaitis,D.N.lJard,and R.T.Ross,J.Cìín. Endocrinol. Metab. 39:451 ,1974
25s Hum, V.G., Knipfel , J.G., and l"lori , K.F., Biochem.13:2359,1g74 Hussa,R.0. n Story,M.T. , and Pattillo, R.A. , J. Ci in. Endocr. Metab. 38: 338 ,1 974 Hichens, 1,1., Grinw.ich, D.1., and Berhman, H.R., progstaglandinsT:449,.1974 Hurley, T.l,l., S. Handwerger, R.E. Fellows, Biochem l6:5598,1977 Hur'ley, T.hI., S. Handwerger, R.E. Fe]1ows, Biochenr. l6:5605,1977 Ikonikoff,L.K. de, C. Hubert, and L. Cedardr, C.R. Acad. Scl. paris, Series D 272:3068,1971 Ikonikoff,L.K, de, and Cedard, 1., A¡n. J.Obstet. Gynecol . ì.|6:1.124,1973 Ingbar, S. H. ,Braverman ,L . E. , Dawber, N.A. , and Lee,G.y., it. .Cl in. Endocr. Metab. 44:l2Bl "ì969 Ito,Y., and Hlgashi "K. Endocr.inol . Jpn, g:279,.l96t Jaffe,R.B, Lee,P.A., and Midgely, A.R.,Jr., J. Clín. Endocr. l,letab. 29:1 281 ,t 969 Josimovich,J.B. and Maclaren,J.A., Endocrinology 71 :209,1962 ilosinovlch, J.B.,B,L. Atwood, and D.A. Goss, Endocrinology 73:4.10,.l963 Josìmovich,J.B., and Brande,B.L., Trans.N,y. Acad. Sci. 27:l6l ,1964 Josimovich, J.B.,Endocrinology 78:707,.l966 .losimovìch, J.8., Endocrinology 83:530,.l969b Josimovich, J.8., Cl.ln. Endocrinol . (N.y.) 2:658,1969a Jungmann,R,A., Hiestand,P,C., and Schweppe, J.S.,Endocrinoìogy94:16g,1974 Jutisz,M., and de 1a L1osa, p., in,'Glycoprotein" (A. Gottschalk, ed), Vol 5, Part 3, pp'|019, flsevier,N.y.,'1972 Ka'lkhoff , Bá(., Richardson,B.L., and Beck,p., Dìabetes l8: .l53,.l969 Kammermann, S., Canfield, R.E., Kolena, J., and Channing,C.p., Endocrinology 91:65,1972 Kann,G., and Denamur, R., J. Reprod. Fert. 392473,1914
- Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
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LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
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NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
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6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
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-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
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-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
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109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
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EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
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SECTION V : RESULTS (r) LACTOGEN DU
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127 Fractioñâtión of oPL. from c
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129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
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TABLE I TABLE OF PURIFICATION OF OV
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137 Figure lì. A, Poìyacrylamide
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Leaf blank to correct nr.rmbering
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14r Anal.vtical Sodi um Dodecyl Sql
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o 9 x F- -)- 9 rU I æ -t f () LT'
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¿ o s : t45 Figure 15b. Displaceme
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HoR MoNE coNc€NrRArto ln ß/mt )
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Tissue used for binding ôtudies Li
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Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
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¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
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P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
- Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
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F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
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! o 't6 l HORMO¡¡ E COr{CENr FAIt
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163 Figure 24 shows the sensltivity
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165 D. STUDIES ON THE SECRETION OF
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E .Dc À o - art & 167 Flgure 26. P
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lJhen blood samples were taken four
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Sheep no. l{6 25í 960 464 5?0 680
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't73 fetal cotylêdons at various d
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TABLE VI OPI CONCENTRATTON IN FETAL
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l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
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dUrlng PrêOnancy in thè Ewes tBr
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! ñ' l83 NoRMON! C oNCENTßArlO þ
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l ol
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I I I "+ _t rl \l :- 'i rl -l l87 a
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l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
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- 193 3H -couNdro _________SPECtFtC
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TABLE IX /- BIOASSAY OF qROIItIfH p
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z o 197 Figure 40, Radioreceptor as
- Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
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SFCT.IoN II : pl.scussr0N 4d ã 1È
- Page 225 and 226: Ch aracteri zati on 203 Analytical
- Page 227 and 228: 205 size, corresponding to the mole
- Page 229 and 230: 207 the specific binding of oPL to
- Page 231 and 232: 209 than with prolactin-like effect
- Page 233 and 234: 2t1 the jncrease in oPRL binding si
- Page 235 and 236: 213 The resuìts obtained indicate
- Page 237 and 238: 215 on the role af PL in the fetus.
- Page 239 and 240: Surgi ca1 method- 217 Our studies o
- Page 241 and 242: 219 5.Relationships amonq opRL. oGH
- Page 243 and 244: 221 The nature of thelarge nolecula
- Page 245 and 246: 223 data argue for a close interact
- Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
- Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
- Page 251 and 252: À) E 229 Figure 43. The secretion
- Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
- Page 255 and 256: 233 Our studies on the blnding of o
- Page 257 and 258: 235 through its binding to cotpus l
- Page 259 and 260: 237 Furthermore, oPL neasurement in
- Page 261 and 262: 239 circulationand alìantoic f'lul
- Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
- Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
- Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
- Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
- Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
- Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
- Page 275: 253 Grinwich,D.L., M. Hichens, and
- Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
- Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
- Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
- Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
- Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
- Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm