THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

More documents

Recommendations

Info

$il\VOLVEMENT OF RETII\OIC ACID II{ - MSpace at the University of ...$

t78 l¡o tóo ,OO t¡O ¡rME rñ MtñufEs Figure 3l . The disappearance of opL fo'llowlng removal of the enfire uterus by caesarian secfion in 3 pregnant ewes at 90 days of gestation. Fromthese data it is possible to calculate that the half_time disappearance rate is approximateìy 50 to 5Z ninutes.
= >€ ,3 ; I 179 rwt 76-0450 o----.------... r¿dro¡:rit'rv rn rhói. btôod twt,t_tôt2 ¡t-- \\ ôl ÌCÂ-rrrolubto n.t.rr!r&-----{ ¡t- - -----¡ r.Ir.ss¡on I'n. $:=qrg=.-=-----___¡ ¡o Tti/E tN MtNUtts Figure 32. semilogarithmic prot of radioactivity (o) of the trlchloro- acetíc acid-insolubre materrar present in r mr serum of pregnant ewes at various lntervals after the administration of l25i-opl. Also p.lotted is the radioactivtty (¡) in 'l ml of whole blood taken at various intervals.
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
Page 3 and 4:
To My parents and wife: Mn & Mrs. K
Page 5 and 6:
CONTTNT S I. REVIEW 0N PLACENTAL PR
Page 7 and 8:
V. RESULTS E. STUDIES ON THT BIOSYN
Page 9 and 10:
Peptide Hormones: ACTH CG FSH GH LH
Page 11 and 12:
LIST OF FIGURES FIGURE PAGE I Linea
Page 13 and 14:
22 Radioreceptor assay for oPRL us.
Page 15 and 16:
Effect of PGF,. and lactogenic horm
Page 17 and 18:
ABSTRACT STUDIES 0N 0VINE PLACENTAL
Page 19 and 20:
eceptor-b i nd i ng studies show th
Page 21 and 22:
-2- years have seen an increasing i
Page 23 and 24:
-4- secretion of a peptide hor:none
Page 25 and 26:
NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
Page 27 and 28:
6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
Page 29 and 30:
-8- 197J-; Rayford et al | !9721 .
Page 31 and 32:
-10- sialic acid díd not alter the
Page 33 and 34:
-L2- and Biasucci (1969) denonstrat
Page 35 and 36:
_14_ addition of CAMP (Handwerger e
Page 37 and 38:
-16- conponents I the.faster haying
Page 39 and 40:
_18_ by hcc, as evidenced by increa
Page 41 and 42:
_20- (Mason et a1 , L973i Menon ¡
Page 43 and 44:
21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
Page 45 and 46:
-23- growth-promoting ef,fect, wher
Page 47 and 48:
-25- diffusion teÊt. Josi¡novich
Page 49 and 50:
-27- v¡hereas in the tissue extrac
Page 51 and 52:
-29- found products of molecular we
Page 53 and 54:
-3r- vÍii) S.ecreLion and metabol
Page 55 and 56:
- 33 - metabolic factors may influe
Page 57 and 58:
-35- Although hpI, si¡nuLates the
Page 59 and 60:
-37- C. CIIHER ITUMAN PLACENTAL PEP
Page 61 and 62:
_39_ stimulating activityf is found
Page 63 and 64:
-4t-- (iii) Human Chorionic Cortlco
Page 65 and 66:
- 43 - syncytiotrophoblast (Tatarin
Page 67 and 68:
- 45 - Ib SUBPRTMATE PLACENTAT PR.O
Page 69 and 70:
-47 - samples, signif,icant elevati
Page 71 and 72:
-49- required for the chorignÍc ao
Page 73 and 74:
B. (i) -51 - MONI(ET PLACENTAL I,AC
Page 75 and 76:
- 53 - brith 13 minutes for hpl,. T
Page 77 and 78:
55 Pregnarit Marê Sêrum Gonâdót
Page 79 and 80:
II b. NON - pRl¡late PT,ACENTAT.
Page 81 and 82:
(iv¡ Inmuhologicat Mêthogs: - 59-
Page 83 and 84:
_ 6t_ 1974; Fellows et a1, 1974¡ H
Page 85 and 86:
63 Ordêr Rodêhtia (Tat, nicê, ro
Page 87 and 88:
Guinea Pi g: 65 Hypophysectomy of t
Page 89 and 90:
-67 Orilet Perissodactyla (Horse an
Page 91 and 92:
-69 activity in the N-acetyl-lactos
Page 93 and 94:
(Íi) coat: -71 The goat is a spont
Page 95 and 96:
- 73- When serum from a pregnant go
Page 97 and 98:
-75 Gusdon et al (1920)using . haer
Page 99 and 100:
77 (c) Imnunologicaì Properties of
Page 101 and 102:
-79 - SECTION II : OYINE REpROpucTI
Page 103 and 104:
BI which contain' as lvell as the o
Page 105 and 106:
83 ce11s usually contaln either num
Page 107 and 108:
85 non-pregnant values did not diff
Page 109 and 110:
8rl Leaf blank to corect nr.rmberin
Page 111 and 112:
- 89- luteolysis when supesistpessd
Page 113 and 114:
9t SECTION IV¡ MtTttODs AND IIATER
Page 115 and 116:
- 93- (.TCA) solution were added co
Page 117 and 118:
-95 - (ÞPRL' NIH-P-B3) , and all q
Page 119 and 120:
97 buffer, the eluates were pooled,
Page 121 and 122:
Char4ctêf ization Pfocedures l. Ch
Page 123 and 124:
t0t (c) À.nalvticaL .{rod.iu¡n do
Page 125 and 126:
103 B : MFTÍÍOD OF DETECTION AND
Page 127 and 128:
AFsa)¡- ProceiiureS 105 Specific b
Page 129 and 130:
107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
Page 133 and 134:
l1ì (wtlvo'). After the hornogenat
Page 135 and 136:
(4) Tissúe distributiori df radloi
Page 137 and 138:
l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
Page 139 and 140:
1.l7 for 30 min. Then the precipita
Page 141 and 142:
(2) Assav of ìuteo.trgpic effec.t
Page 143 and 144:
EXPERIMENT # TI (7 days after HCG)
Page 145 and 146:
123 to progesterone (l/300 djlution
Page 147 and 148:
SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150: 127 Fractioñâtión of oPL. from c
Page 151 and 152: 129 Figure 6. carborymethyì(cM,)-c
Page 153 and 154: t 3't óo0 ,oo 800 900 FRACIION r,t
Page 155 and 156: E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158: TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160: 137 Figure lì. A, Poìyacrylamide
Page 161 and 162: Leaf blank to correct nr.rmbering
Page 163 and 164: 14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166: o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168: ¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170: HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172: Tissue used for binding ôtudies Li
Page 173 and 174: Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176: ¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178: P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180: o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182: F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184: ! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186: 163 Figure 24 shows the sensltivity
Page 187 and 188: 165 D. STUDIES ON THE SECRETION OF
Page 189 and 190: E .Dc À o - art & 167 Flgure 26. P
Page 191 and 192: lJhen blood samples were taken four
Page 193 and 194: Sheep no. l{6 25í 960 464 5?0 680
Page 195 and 196: 't73 fetal cotylêdons at various d
Page 197 and 198: TABLE VI OPI CONCENTRATTON IN FETAL
Page 199: l. Súrglcat Method 't77 Figure 3l
Page 203 and 204: dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206: ! ñ' l83 NoRMON! C oNCENTßArlO þ
Page 207 and 208: l ol
Page 209 and 210: I I I "+ _t rl \l :- 'i rl -l l87 a
Page 211 and 212: l89 Flgure 37. Percentage ot l2sl-o
Page 213 and 214: 19t column is shown in Figure 3BA.
Page 215 and 216: - 193 3H -couNdro _________SPECtFtC
Page 217 and 218: TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220: z o 197 Figure 40, Radioreceptor as
Page 221 and 222: 5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224: SFCT.IoN II : pl.scussr0N 4d ã 1È
Page 225 and 226: Ch aracteri zati on 203 Analytical
Page 227 and 228: 205 size, corresponding to the mole
Page 229 and 230: 207 the specific binding of oPL to
Page 231 and 232: 209 than with prolactin-like effect
Page 233 and 234: 2t1 the jncrease in oPRL binding si
Page 235 and 236: 213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252:
À) E 229 Figure 43. The secretion
Page 253 and 254:
231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256:
233 Our studies on the blnding of o
Page 257 and 258:
235 through its binding to cotpus l
Page 259 and 260:
237 Furthermore, oPL neasurement in
Page 261 and 262:
239 circulationand alìantoic f'lul
Page 263 and 264:
SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266:
SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268:
Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270:
247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272:
249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274:
25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276:
253 Grinwich,D.L., M. Hichens, and
Page 277 and 278:
25s Hum, V.G., Knipfel , J.G., and
Page 279 and 280:
?57 Knopp,R,H,, H,J. Ruder,E. Herra
Page 281 and 282:
259 Manku,M.S., and D.F. Horrobin,
Page 283 and 284:
261 , :iir .,.-;;r-,:, Morgan, F.J.
Page 285 and 286:
263 Pierce,G.B., Midþley,A.R. and
Page 287 and 288:
?65 Shepard, T.H.., Gen. Comp. Endo
Page 289 and 290:
267 Tsuruhara,T., Dufau,M.L., Hickm
show all

THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

Create successful ePaper yourself

Delete template?

Save as template?