252 Genbacev,0., and Sulovic, V., Cytoblologie LL:96,197b Geschwind,I.I. and Dewey,R., proc. Soc. Exp. Biol . Med. 129:451 ,.l96g Gibbons, J.M., M.itnick,M., and Chieffo,V., Am. J.0bstet.Gynecol . ì211.l27,1975 Git'lin,D., and Biasucc.i,A. , J.Clln Endocr. Metab. 29:926,.l969 Gonzaìez-Angul ,4., Hernandez -Jauregu.i ,p. , and Marquez_Monter,H., Amer.J. Vit. Res. 32:.l661 ,1971 Got,R.,and Boumiì lon,R., Biochim.Biophys. Acta 42:505,1960 Gospodarowicz,D. , Endocrinology 9.1 :l0l ,.1972 Goding, J.R., Buchmaster, J.M., Cerìnl ,J.C., Cer.inì,M.E.0. ,Chamìey,lJ.A., ' Fell,L.R., F.indley,J.K., and Jones,H., J. Reprod Fert. suppl lB:31 ,1973 Goding,J.R., Catt,K.J., Brown,J.M., Ka'ltenbach,C,C.,Cunning, I.A., and l'1ole,B.J., Endocrinoìogy 85:.l33,1969 Goverde,B,C.,Veenkamp,F.il. N., and Homan,J. D.H.,Acta Endocrino.l . ( Copperhagen) 59:105,1968 Greenblatt,D., and J. Koch-lleser,N.Engt. J. Med. 293:702,.l97S Greenblatt,D., and rl. Koch-Weser, N.Engl . J. Med. 293:g64,l9lï Graess'l in,D.,l,leise,H.C., and Braendle,trl., FEBS Lett.3ì,214,1g7J Grant,D. B.,S.L, Kapìan,and M.M, Grumbanch,Acta Endocr. 63 : 736,.l 970 Gudson,J.P,,Leake,N.H.,van Dyke, A.H. and ll.AtkJns,Am. J. Obstet and Gynec. 104:441 ,1970 Grumbach,M.M., Kaplan,S.L., Trans. N.y. Acad. Sci. 27:.l67,.1964 Grumbach. l'l. l'l.,Kaplan,S.L., Abrams,C.L.,Bel.l,J. J., and Conte,F.A., J. ClÍn Endocr. Metab.26:478,ì966 Grumbach,M.M., Kaplan,S.L., Sciarra,J.J., and Burr,I.M., Ann. N.y. Acad. Sci. 148:501,'1968
253 Grinwich,D.L., M. Hichens, and H.R. Hehrman,Bloì. Reprod. 14:212,1976 Grinwich,D.L. , Ham,E.A. ,Hichens, and [i.R. Berhman,Fed. proc.34:260,ì97s Grissom, Gunkeet,D.'Merz'w.E.,Hr'lgenfert,u.,añd Brossmer,R:,FEBs Lett.b3:309.1975 $udson,J.P. " .Jr., and Yen,S.S.C.,.obstet. eynecót-.iO:OlS.lSOi ' Gusdon,J.P.,Leake,Þl.H,..,van_Dykg,A.H. and.l.rl..AtHns, Am,. rl.. Obstet and Gynec. 104:441 ,1970 . Halban,,J., Ârch Gynaek Z5-:353,ì905 Ha]pin,T.F., Am. J. Obstet. Gynecol . ì06:3ì7,1970 llambl ey,J.,and Grant,D.B., Acta Endocrinol. Coppenhagen 70: 43,1912 fi *ln!fu 8¡Ts? ;'åiE'sr,È¡"å[üllîi, : lt ?;u[å¿HI;.i. Honnones, Feta 1 NutritÍon and Lactailon, (J.8. Josimóvich,ed),pp33_47, l'liley, N.y.,ì974 Handwerger,s" ,pang,E.c. , Aìoi,s.M. , and sherwood,L.M. ,Endocrinorogy 91 :7Zl ,.1972 Handwerger,s.,Barrett,J.,Tyrey,L., and schomberg,D. ". J. cì in. Endocr. Metab. 36 : 'l 268 ,1973 Handwerger, S., Maurer, Hurley,T., Barrett, J. and R.E. Feììows, Endocrine Res. Cormun l:403,ì974 Handwerger, s., c. crenshaw,Jr., r.,lF.. Maurer,.J. Barrett, T.!J. Hurrey, Golander, and R.E. Felìows, ,J. Endocrinol ogy lZ:27,1977 Handwerger"S., R.E. Fellows,M.C., Crenshaw, T. Hurley, ,1. Barrett, and W.G. Maurer, ,J. Endocr. 69:133,.lg76 Harkness, D.R., Arch. Biochem. Biophys. 126:503,.I968 ,Ïå,få'Slrt.'crui ; oË30';.i 3i ; r6ðf i rzt¿. t I i ¡r. v. I +ir' ããl, I s c I Harrison,F.A., and R.B. Heap, J. physiol .l96:43,196g Heap,R.B. and R. Deanesly,R., J. Endocr 30:j-iii,l964 Heap,R.B. and R. Deanesly, .1. Endocr 34:4t7,1966
- Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
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LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
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NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
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6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
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-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
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-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
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Char4ctêf ization Pfocedures l. Ch
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t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
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AFsa)¡- ProceiiureS 105 Specific b
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107 ', C : IIEBI{OÐ OF DEVELOPMENT
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109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
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l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
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EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
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SECTION V : RESULTS (r) LACTOGEN DU
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127 Fractioñâtión of oPL. from c
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129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
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TABLE I TABLE OF PURIFICATION OF OV
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137 Figure lì. A, Poìyacrylamide
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Leaf blank to correct nr.rmbering
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14r Anal.vtical Sodi um Dodecyl Sql
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o 9 x F- -)- 9 rU I æ -t f () LT'
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¿ o s : t45 Figure 15b. Displaceme
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HoR MoNE coNc€NrRArto ln ß/mt )
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Tissue used for binding ôtudies Li
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Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
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¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
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P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
- Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
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F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
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! o 't6 l HORMO¡¡ E COr{CENr FAIt
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163 Figure 24 shows the sensltivity
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165 D. STUDIES ON THE SECRETION OF
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E .Dc À o - art & 167 Flgure 26. P
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lJhen blood samples were taken four
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Sheep no. l{6 25í 960 464 5?0 680
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't73 fetal cotylêdons at various d
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TABLE VI OPI CONCENTRATTON IN FETAL
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l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
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dUrlng PrêOnancy in thè Ewes tBr
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! ñ' l83 NoRMON! C oNCENTßArlO þ
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l ol
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I I I "+ _t rl \l :- 'i rl -l l87 a
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l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
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- 193 3H -couNdro _________SPECtFtC
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TABLE IX /- BIOASSAY OF qROIItIfH p
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z o 197 Figure 40, Radioreceptor as
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5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
- Page 223 and 224: SFCT.IoN II : pl.scussr0N 4d ã 1È
- Page 225 and 226: Ch aracteri zati on 203 Analytical
- Page 227 and 228: 205 size, corresponding to the mole
- Page 229 and 230: 207 the specific binding of oPL to
- Page 231 and 232: 209 than with prolactin-like effect
- Page 233 and 234: 2t1 the jncrease in oPRL binding si
- Page 235 and 236: 213 The resuìts obtained indicate
- Page 237 and 238: 215 on the role af PL in the fetus.
- Page 239 and 240: Surgi ca1 method- 217 Our studies o
- Page 241 and 242: 219 5.Relationships amonq opRL. oGH
- Page 243 and 244: 221 The nature of thelarge nolecula
- Page 245 and 246: 223 data argue for a close interact
- Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
- Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
- Page 251 and 252: À) E 229 Figure 43. The secretion
- Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
- Page 255 and 256: 233 Our studies on the blnding of o
- Page 257 and 258: 235 through its binding to cotpus l
- Page 259 and 260: 237 Furthermore, oPL neasurement in
- Page 261 and 262: 239 circulationand alìantoic f'lul
- Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
- Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
- Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
- Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
- Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
- Page 273: 25t Friesen,H.G., Endocrinoìogy 76
- Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
- Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
- Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
- Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
- Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
- Page 287 and 288: ?65 Shepard, T.H.., Gen. Comp. Endo
- Page 289 and 290: 267 Tsuruhara,T., Dufau,M.L., Hickm