THE UNIVERSITY OF MANITOBA STUDIES ON OVINE PLACINTAL ...

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266 Story'M.T., Hussa,R.0., and pattiì1o,R.A., J. cl.ln. Endocr. l,4etab. 39:g77,.197 SussmEn,H. H., and Gottl ieb,A. J., B.ioch.im. B.i ophys. Acta. I 94 : 1 70,.1 969 Suwa,S., and Friesen,H., Endocrinology BS:1082,ì969a Suwa,S., and Friesen,H.G.,Endocrinology g5:.|037,.1969b swamìnathan,N., and Bahr,0.p., Biochem. Biophys, Re. con¡nun.40 :422,.1970 Talarnates,F., the S5th nreeting of the Endocrine Society,Chicago,June,.lgT3 Talwar,G.P., Sharma,N.C., Dubey,S.K., Salahuddin,M., Das,C., Ramakrishnan,S., Kumar,S., and Hingoranl ,V., proc. Nail. Acad. Sci. U.SA 73:2:/8,1976 Tatar'lnov,Y. S., Fal aleeva,D. M., Kalashnì kov,V. V., and To.l oknov,B.0., Natu re (London )260:263 ,1976 Teasdaìe,F. , Adcock,E.l,l. , III,August,C.S. , Cox,S. , Battagl ia,F.C. , and I'laughton, M.A.,Gynecoì. Inve st.4:263,.l973 Thompson,F.N., and l^lagner,}J.C., J. Reprod. Fert. 4l:57,1914a Thompson,F.N., and l,lagner,l^l.C.,tl. Reprod. Fert. 4.1. :49,,l974b Thorel l,J. I. and B.c. Johannson,Bjochem.Biophys. Acta 25,1 :363,.l 97.l Tindal ,J.S. " J. Reprod. Fert. 39:436,ì974 Tojo,S. , Kanazawa,S. , Nakamura,A. , Kitagaki ,S. ,and Mochizuki,M. , Endocrinol . Jpn. 20:505,.l973 Tojo's.,MochizukÍ,M., and Maruo,T., ln..Goñadótrôpihs. and Gonadar Function (N.R. Moudgal ,ed),p321 .Acad. press,N.y. , .l974 Tojo,S., Ashitaka,y., Maruo,T., and Nishimoto,H., Endocr. Japn.22:S85,ì975 Tuì l ner,ü'1. , and Hertz,R.,Endocrino.logy 781204,1966a Tuìlner,lrl., and Hertz,R., Endocrinology 7g:ì076,1966b Tul'lner,W., Endocrinoìogy gZ:874,1g6g Tuì lner,lnl., in Lund c.J. and choate,J.ll.,Eds., TransacHons of the Fifth Rochester Trophoblast Conf. p363,.l969 Tul I ner,l'l., Rayford,p.L., and Ross,G.T., Endocrlnologyg4 :90g, .l969
267 Tsuruhara,T., Dufau,M.L., Hickman,il., and catt,K.J., Endocrlno.logy 9l : 296,1972a Tsuruhara,T., van Hal'l ,E.V., Dufau,M.L., and Catt,K.J., Endocrinoìogy9l: 463,1972b Tsushima,T.and H.G. Friesen, J. Clin tndocr. Metab. 37:334,.|973 Turkington,R.l^l.,J.Ctin. tndocr. Metab. 33:2ì0,ì971 Tbrtle,J.R., Kìpnis,D.M.,Biochim. Biophys. Acta .|44:583,1967 Turtl e,,J. R., Beck,P., and Daughada.y,ü1.H., Endocrinol ogy 79 : l g7,ì 966 Tyson,J.t., Felder,A.J., Austin,K.L., and Farinholt,,r., in The placenta: Biologicaì and Clinical Aspects',(K.S. Moghissì and E.S.E. Hafez, eds. )pp 275, Thomas,sprlngf.ield, Illinois.,.l974 Vaitukaitis,J,L., Ross,G.T., Re.ichert,L.E. , Jr., and Ward,D.N., Endocr.inoìog3 91,:1337 ,1972 Vaitukaitis,J.L.,Lee,C.Y., Ebersole,E.R., and Lerarlo,A.C., Endocrinology 97:215.1975 Vaitukaitis,J.L., Ross,G.T., Braunstein,G.D., and Rayford,p.L., Recent Prog. Horm. Res. 32:289,1976 Van Ha'lì,E.V., Vaitukaitis,J.L., Ross,G.T.,Hickman,J.l,l.,and Ashwell,G., Endocrinol ogy 88: 456,1 97.l a Van Hall,t.V., Vaitukaitis,J.L.,Ross,G.T., Hickman,J.ü,1., and Ashwell,G., Endocrinology 89: l t,l97lb Van Hell, H., and Schuurs,A.H.ht.M., Gonadotropìns 0varian Dev., proc. Workshop Meet. p70-76,19690970) Van Rensburg,S.J., 0nderstepoort,J., Vet. Res. 3g:t,l97J Vi 1 lee,C.A,, and Gabbe,S., in Gonadotropins (8. B. Saxena rC.G. Beì i ng,and H. M.Gandy,Eds. ),p309,}li1ey(Interscience),N. y., 197ì
Page 1 and 2:
THE UNIVERSITY OF MANITOBA STUDIES
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To My parents and wife: Mn & Mrs. K
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CONTTNT S I. REVIEW 0N PLACENTAL PR
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V. RESULTS E. STUDIES ON THT BIOSYN
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Peptide Hormones: ACTH CG FSH GH LH
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LIST OF FIGURES FIGURE PAGE I Linea
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22 Radioreceptor assay for oPRL us.
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Effect of PGF,. and lactogenic horm
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ABSTRACT STUDIES 0N 0VINE PLACENTAL
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eceptor-b i nd i ng studies show th
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-2- years have seen an increasing i
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-4- secretion of a peptide hor:none
Page 25 and 26:
NHt-Aì a-Pro-Asp-Val -Gl n-Asp-Cys
Page 27 and 28:
6a NH, -Ser-Lys -G'l u.Pro -Leu -Ar
Page 29 and 30:
-8- 197J-; Rayford et al | !9721 .
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-10- sialic acid díd not alter the
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-L2- and Biasucci (1969) denonstrat
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_14_ addition of CAMP (Handwerger e
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-16- conponents I the.faster haying
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_18_ by hcc, as evidenced by increa
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_20- (Mason et a1 , L973i Menon ¡
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21a NH, -Vaì -G1 n-Thr-Val -Pro-Le
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-23- growth-promoting ef,fect, wher
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-25- diffusion teÊt. Josi¡novich
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-27- v¡hereas in the tissue extrac
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-29- found products of molecular we
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-3r- vÍii) S.ecreLion and metabol
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- 33 - metabolic factors may influe
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-35- Although hpI, si¡nuLates the
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-37- C. CIIHER ITUMAN PLACENTAL PEP
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_39_ stimulating activityf is found
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-4t-- (iii) Human Chorionic Cortlco
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- 43 - syncytiotrophoblast (Tatarin
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- 45 - Ib SUBPRTMATE PLACENTAT PR.O
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-47 - samples, signif,icant elevati
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-49- required for the chorignÍc ao
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B. (i) -51 - MONI(ET PLACENTAL I,AC
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- 53 - brith 13 minutes for hpl,. T
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55 Pregnarit Marê Sêrum Gonâdót
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II b. NON - pRl¡late PT,ACENTAT.
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(iv¡ Inmuhologicat Mêthogs: - 59-
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_ 6t_ 1974; Fellows et a1, 1974¡ H
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63 Ordêr Rodêhtia (Tat, nicê, ro
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Guinea Pi g: 65 Hypophysectomy of t
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-67 Orilet Perissodactyla (Horse an
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-69 activity in the N-acetyl-lactos
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(Íi) coat: -71 The goat is a spont
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- 73- When serum from a pregnant go
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-75 Gusdon et al (1920)using . haer
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77 (c) Imnunologicaì Properties of
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-79 - SECTION II : OYINE REpROpucTI
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BI which contain' as lvell as the o
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83 ce11s usually contaln either num
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85 non-pregnant values did not diff
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8rl Leaf blank to corect nr.rmberin
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- 89- luteolysis when supesistpessd
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9t SECTION IV¡ MtTttODs AND IIATER
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- 93- (.TCA) solution were added co
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-95 - (ÞPRL' NIH-P-B3) , and all q
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97 buffer, the eluates were pooled,
Page 121 and 122:
Char4ctêf ization Pfocedures l. Ch
Page 123 and 124:
t0t (c) À.nalvticaL .{rod.iu¡n do
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103 B : MFTÍÍOD OF DETECTION AND
Page 127 and 128:
AFsa)¡- ProceiiureS 105 Specific b
Page 129 and 130:
107 ', C : IIEBI{OÐ OF DEVELOPMENT
Page 131 and 132:
109 D. RTNG PREGNôXCY (l) Maternâ
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l1ì (wtlvo'). After the hornogenat
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(4) Tissúe distributiori df radloi
Page 137 and 138:
l'1 5 (2) Procg.ssjnq, gf. incuÞ¡
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1.l7 for 30 min. Then the precipita
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(2) Assav of ìuteo.trgpic effec.t
Page 143 and 144:
EXPERIMENT # TI (7 days after HCG)
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123 to progesterone (l/300 djlution
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SECTION V : RESULTS (r) LACTOGEN DU
Page 149 and 150:
127 Fractioñâtión of oPL. from c
Page 151 and 152:
129 Figure 6. carborymethyì(cM,)-c
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t 3't óo0 ,oo 800 900 FRACIION r,t
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E o ø ô¡ t- z ao É, o '133 .--.
Page 157 and 158:
TABLE I TABLE OF PURIFICATION OF OV
Page 159 and 160:
137 Figure lì. A, Poìyacrylamide
Page 161 and 162:
Leaf blank to correct nr.rmbering
Page 163 and 164:
14r Anal.vtical Sodi um Dodecyl Sql
Page 165 and 166:
o 9 x F- -)- 9 rU I æ -t f () LT'
Page 167 and 168:
¿ o s : t45 Figure 15b. Displaceme
Page 169 and 170:
HoR MoNE coNc€NrRArto ln ß/mt )
Page 171 and 172:
Tissue used for binding ôtudies Li
Page 173 and 174:
Noñ. p..s'ro¡r {N,p.l Pr.eñonr (
Page 175 and 176:
¡( É T 2, \ ¡a.2¡./ir ,..r.¡.
Page 177 and 178:
P 80 \ x.. ¡,azrot¡j .1.¿¡i¡ .
Page 179 and 180:
o c o U o òs -o c2o ao eÀo o 'o .
Page 181 and 182:
F 2 o o o\ ¡¡ig ô 2 Þ 0 (¡ l5e
Page 183 and 184:
! o 't6 l HORMO¡¡ E COr{CENr FAIt
Page 185 and 186:
163 Figure 24 shows the sensltivity
Page 187 and 188:
165 D. STUDIES ON THE SECRETION OF
Page 189 and 190:
E .Dc À o - art & 167 Flgure 26. P
Page 191 and 192:
lJhen blood samples were taken four
Page 193 and 194:
Sheep no. l{6 25í 960 464 5?0 680
Page 195 and 196:
't73 fetal cotylêdons at various d
Page 197 and 198:
TABLE VI OPI CONCENTRATTON IN FETAL
Page 199 and 200:
l. Súrglcat Method 't77 Figure 3l
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= >€ ,3 ; I 179 rwt 76-0450 o----
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dUrlng PrêOnancy in thè Ewes tBr
Page 205 and 206:
! ñ' l83 NoRMON! C oNCENTßArlO þ
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l ol
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I I I "+ _t rl \l :- 'i rl -l l87 a
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l89 Flgure 37. Percentage ot l2sl-o
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19t column is shown in Figure 3BA.
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- 193 3H -couNdro _________SPECtFtC
Page 217 and 218:
TABLE IX /- BIOASSAY OF qROIItIfH p
Page 219 and 220:
z o 197 Figure 40, Radioreceptor as
Page 221 and 222:
5A L lNl Pgtzq cô-tlzt Pgfl6 + cl
Page 223 and 224:
SFCT.IoN II : pl.scussr0N 4d ã 1È
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Ch aracteri zati on 203 Analytical
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205 size, corresponding to the mole
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207 the specific binding of oPL to
Page 231 and 232:
209 than with prolactin-like effect
Page 233 and 234:
2t1 the jncrease in oPRL binding si
Page 235 and 236:
213 The resuìts obtained indicate
Page 237 and 238: 215 on the role af PL in the fetus.
Page 239 and 240: Surgi ca1 method- 217 Our studies o
Page 241 and 242: 219 5.Relationships amonq opRL. oGH
Page 243 and 244: 221 The nature of thelarge nolecula
Page 245 and 246: 223 data argue for a close interact
Page 247 and 248: ]. TABLE OF CHEMICAL PROPERTIES GUI
Page 249 and 250: cor,t coAT (bPL) (cpl) 3. TABLE OF
Page 251 and 252: À) E 229 Figure 43. The secretion
Page 253 and 254: 231 H. POSSIBLE ROLT OF OVINE PLACE
Page 255 and 256: 233 Our studies on the blnding of o
Page 257 and 258: 235 through its binding to cotpus l
Page 259 and 260: 237 Furthermore, oPL neasurement in
Page 261 and 262: 239 circulationand alìantoic f'lul
Page 263 and 264: SECTICN IX PUBLICATIONS A) Manuscr
Page 265 and 266: SECTION X: BIBLIOGRAPHY Abramovich,
Page 267 and 268: Beas,F., Saìinas, 4., GonzalezrF.,
Page 269 and 270: 247 Brody,S,, and Carlstrom,G.,J.Cl
Page 271 and 272: 249 Crosignanì ,P.G. , Nencioni ,
Page 273 and 274: 25t Friesen,H.G., Endocrinoìogy 76
Page 275 and 276: 253 Grinwich,D.L., M. Hichens, and
Page 277 and 278: 25s Hum, V.G., Knipfel , J.G., and
Page 279 and 280: ?57 Knopp,R,H,, H,J. Ruder,E. Herra
Page 281 and 282: 259 Manku,M.S., and D.F. Horrobin,
Page 283 and 284: 261 , :iir .,.-;;r-,:, Morgan, F.J.
Page 285 and 286: 263 Pierce,G.B., Midþley,A.R. and
Page 287: ?65 Shepard, T.H.., Gen. Comp. Endo
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